Publications by authors named "Mansuy J"
Background: Severe acute respiratory syndrome coronavirus 2, the virus responsible for coronavirus disease 2019, affects multiple organs. The virus enters cells through angiotensin-converting enzyme-2 and host factors present in genital organs, leading to concern over virus shedding in semen and reproductive function.
Objectives: To investigate severe acute respiratory syndrome coronavirus 2 in semen from patients with a mild infection, identify the seminal infected cells, and explore the effect of the infection on sex hormones and semen parameters.
Introduction: Inflammation related to influenza virus infection can lead to multiple neurological presentations. Encephalitis is one of them, mostly accompanied by seizures, with different profiles depending on the epidemics and previous medical conditions.
Materials And Methods: All children presenting neurological symptoms and positive for influenza virus RNA detection in a respiratory sample between November 2018 and April 2023, hospitalized in the Department of Paediatric Neurology of Toulouse Children's Hospital, were retrospectively analysed.
Introduction: As there is no specific antiviral treatment currently available for BK polyomavirus associated nephropathy (BKVAN), its management relies on immunosuppression reduction in kidney transplant patients. Data on efficacy of steroid pulses in this indication are lacking.
Methods: We performed a retrospective monocenter study on 64 patients diagnosed with biopsy-proven BKVAN.
Article Synopsis
- * A retrospective study at Toulouse University Hospital from 2008 to 2018 found no decrease in NP incidence, with 56% of cases showing bacteria, mainly Streptococcus pneumoniae and Staphylococcus aureus.
- * The study observed changes in bacterial patterns related to the vaccine's introduction, highlighting an increase in S. pyogenes and S. aureus cases, indicating the need for ongoing research and monitoring of these changes.
A patient aged 28 years who is immunocompromised and living with HIV/AIDS became infected with the monkeypox virus (MPXV). His clinical condition deteriorated for 37 days, with fever, skin lesions, and diarrhea before going to the infectious diseases department, where his severe, protracted infection was treated with tecovirimat for 14 days. His condition rapidly improved, and the skin lesions decreased, as did the MPXV loads, with no adverse events.
View Article and Find Full Text PDFThe Omicron variant differs from earlier strains of SARS-CoV-2 in the way it enters host cells and grows . We therefore reevaluated its diagnosis using saliva, nasopharyngeal swab (NPs), and anterior nasal swab (ANs) specimens from 202 individuals (64.9% symptomatic) tested at the Toulouse University Hospital SARS-CoV-2 drive-through testing center.
View Article and Find Full Text PDFArticle Synopsis
- - The study explores how human cytomegalovirus (hCMV) affects small extracellular vesicles (sEVs) produced by first trimester placenta cells, revealing an increase in the quantity and changes in protein content of sEVs following infection.
- - Researchers used various techniques, including biochemistry and microscopy, to demonstrate that sEVs from hCMV-infected cells enhance infection in fetal-derived cells, suggesting a mechanism for viral spread.
- - The findings imply that placental sEVs may play a crucial role in facilitating the transmission of hCMV to the fetal brain during early stages of pregnancy.
Background And Aims: Direct virological diagnosis of Severe Acute Respiratory Syndrome CoronaVirus 2 (SARS-CoV-2) infectionis based on either viral antigen or viral genome detection. These methods, in addition to the dedicated reagents and transport packaging, require the use of quantities of plastic that may individually appear negligible but which, in the context of a pandemic, are very high. The aim was to estimate the amount of plastic involved in a diagnostic assay whether molecular or antigenic.
View Article and Find Full Text PDFArticle Synopsis
- - We studied the evolution of Omicron sublineages of the SARS-CoV-2 virus from December 2021 to mid-March 2022 using a specific type of PCR method.
- - The research focused on comparing the BA.2 variant to the BA.1 variant to understand their dynamics during this period.
- - Findings indicate that BA.2’s advantage over BA.1 isn't linked to higher amounts of the virus found in the nasopharynx.
We investigated the effects of dengue virus (DENV) on semen using samples collected 7, 15, 30, 60, and 90 days after symptom onset from 10 infected volunteers on Réunion Island. We assessed characteristics of semen and reproductive hormones and isolated motile spermatozoa from semen. We assayed semen for DENV using reverse transcription PCR and searched for DENV RNA by virus isolation in Vero E6 cell cultures.
View Article and Find Full Text PDFArticle Synopsis
- A study conducted at Toulouse University Hospital analyzed the nasopharyngeal viral load (VL) of SARS-CoV-2 in 3775 patients infected with Delta and Alpha variants, focusing on how vaccination status impacted these levels.
- The findings revealed that Delta variant infections had significantly higher VL compared to Alpha infections, particularly in patients diagnosed later after symptom onset.
- Vaccinated individuals showed lower VL regardless of the variant, indicating that vaccination could help reduce viral spread, especially with the more contagious Delta variant.
Objective: To describe the contribution of the rapid antigen diagnostic testing (RDT) to the management strategy of the COVID-19 pandemic.
Methodologies: The protein chain reaction (PCR) and the RDT have been performed on each COVID-19 suspected workers from December 2020 to September 2021.
Results: A total of 286 people tested.
Article Synopsis
- Extracellular vesicles (EVs) play important roles in various physiological and disease conditions, including pregnancy, serving as biomarkers and communicators between the placenta and both mother and fetus.
- The study focuses on isolating small EVs from first trimester placental explants, investigating both normal conditions and those affected by human cytomegalovirus infection.
- Findings revealed that infection alters several surface marker expressions but does not impact EV secretion or integrity, setting the stage for understanding EV functions in early pregnancy and identifying new biomarkers for congenital infections.
Background: The recent emergence of new SARS CoV-2 variants (variants of concern, VOC) that spread rapidly and may lead to immune escape has emphasized the urgent need to monitor and control their spread.
Methods: We analyzed 2018 SARS-CoV-2 positive specimens collected between February 9 and March 22, 2021 using the Thermofisher® TaqPath™ COVID-19 CE-IVD RT-PCR kit (TaqPath) and the ID solutions® ID™ SARS-CoV-2/UK/SA Variant Triplex RT-PCR (ID triplex) assay to screen for VOCs.
Results: The ID triplex assay identified 62.
Testing individual and pooled saliva samples for sars-cov-2 nucleic acid: a prospective study.
Diagn Microbiol Infect Dis
November 2021
Control of the rapid spread of the SARS-CoV-2 virus requires efficient testing. We collected paired nasopharyngeal swab (NPs) and saliva samples from 303 subjects (52.8% symptomatic) at a drive-through testing center; 18% of whom tested positive.
View Article and Find Full Text PDFArticle Synopsis
- SARS-CoV-2 and COVID-19 have resulted in over 2.6 million deaths by March 2021, prompting studies on the virus's spread.
- A modified SIR epidemiological model was used to forecast the virus's spread in France, impacted by different variants and public health strategies, including vaccinations.
- The B.1.1.7 variant significantly increased in Toulouse, but testing rates didn't rise, indicating that ongoing public health measures and faster vaccination are key to controlling the virus.
Article Synopsis
- SARS-CoV-2, the virus causing COVID-19, has resulted in over 2 million deaths globally as of January 2021.
- A modified epidemiological model was used to predict how the virus will spread in France based on different public health strategies, including vaccination.
- Predictions suggest that slowly lifting lockdowns could lead to virus levels similar to pre-lockdown times, but a strong vaccination campaign combined with ongoing health measures can help reduce virus spread and lessen hospital strain.