Efficient biosorption of Zn(II), Cd(II), and Pb(II) by Aspergillus brasiliensis in industrial wastewater coupled with electrochemical monitoring via sensor enhanced with modified silver nanoparticles.

This work investigates the use of Aspergillus brasiliensis, this particular species of Aspergillus, as a biosorbent for the first time. It is employed to biosorption Zn(II), Cd(II), and Pb(II) and combines the biosorption experiments with electrochemical measurements for in situ analysis. For the experiments, a batch system was employed with the dead biomass.

Advances in Detecting Cystic Echinococcosis in Intermediate Hosts and New Diagnostic Tools: A Literature Review.

Cystic echinococcosis (CE) is a zoonotic disease affecting humans and animals. Despite a lack of clarity about many details of parasite-intermediate host interactions, the nature of the immune responses triggered by hydatid infection has revealed new perspectives. This study discusses the latest advances in elucidating the immunologic mechanism of echinococcosis and its detection and potential approaches to enhance serodiagnosis accuracy.

Effect of new methionine-based ionic liquid on the and gene expression on sensitive and resistant strains of .

Background And Objectives: Previous researchers showed the antimicrobial ability of ionic liquids (ILs) on different infective agents. ILs can dissolve organic components, especially DNA molecules. Among synthesized eight binary ILs mixtures, we have chosen ([Met-HCl] [PyS]) IL for determining the antifungal ability of IL against cells.

Antimicrobial and anti-biofilm effects of carotenoid pigment extracted from strain on food-borne bacteria.

Background And Objectives: Carotenoid pigments are among the most important pigments and have many applications in various food, cosmetics, hygiene industries and biotechnology. These pigments are produced by plants and microorganisms including spp. This research intended to study the antimicrobial and antibiofilm effects of the carotenoid pigment from on food spoilage bacteria ( and Typhimurium).

Molecular study and antifungal susceptibility profile of and strains isolated from lesions of humans and cattle.

Background And Objectives: Monitoring of contagious diseases is important to advance our knowledge of their epidemiology and to enable more impressive investigation and prevention efforts. This study aimed to examine antifungal drug susceptibility and molecular analysis of clinical isolates of and in humans and cattle.

Materials And Methods: A total of 400 patients and 500 cattle were evaluated in this study.

Naked-Eye Detection of Morphine by Au@Ag Nanoparticles-Based Colorimetric Chemosensors.

In this study, we report a novel and facile colorimetric assay based on silver citrate-coated Au@Ag nanoparticles (Au@AgNPs) as a chemosensor for the naked-eye detection of morphine (MOR). The developed optical sensing approach relied on the aggregation of Au@Ag NPs upon exposure to morphine, which led to an evident color variation from light-yellow to brown. Au@Ag NPs have been prepared by two different protocols, using high- and low-power ultrasonic irradiation.

Effect of fluconazole and terbinafine nanoparticles on the treatment of dermatophytosis induced by in guinea pig.

Background And Objectives: Dermatophytosis induced by is a major human and animal fungal contamination. Antifungals like terbinafine and fluconazole are widely used to treat dermatophytosis; nevertheless, the prevalence of drug resistance has increased. Hence, novel curative strategies are needed.

In-Vitro Activity of Nano Fluconazole and Conventional Fluconazole against Clinically Important Dermatophytes.

Background: Dermatophytosis is one of the most common fungal infections in humans. Antifungals such as fluconazole are effectively used for treating dermatophytosis; however, drug resistance was observed in many cases. Therefore, a newer treatment strategy is essential.

Validation of an UHPLC-MS/MS Method for Simultaneous Analysis of 11 Mycotoxins in Wheat Flour Using Immunoaffinity Column.

This study focuses on optimization and validation of an Ultrahigh-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) method for simultaneous analysis of 11mycotoxins: Aflatoxins (B, B, G, and G), Ochratoxin A, Deoxynivalenol, Fumonisins (B and B), Zearalenone, T-2, and HT-2toxin, in wheat matrix. Sample extraction and cleanup procedure is based on a single extraction step using acetonitrile/water/acetic acid mixture (79.5/20/0.

Absence of AfuXpot, the yeast Los1 homologue, limits Aspergillus fumigatus growth under amino acid deprived condition.

In Saccharomyces cerevisiae, los1 encodes a nuclear tRNA exporter. Despite the non-essentiality, the deletion of los1 has been shown to extend replicative life span in yeast. Here, we characterized AfuXpot, the los1 homologue in human pathogen Aspergillus fumigatus and found that it is continuously expressed during fungal growth.

The assessment of anti-tumoral activity of polysaccharide extracted from terrestrial filamentous fungus.

Fungal polysaccharides are well-known for the medicinal properties such as antitumor and immunomodulating effects. Hence, this study evaluated antitumor effects of polysaccharide extracted from sp. isolated from soil samples of Karaj district, Alborz, Iran along with its taxonomic study.

Epithelial mesenchymal transition Transcription Factor (TF): The structure, function and microRNA feedback loop.

Epithelial to mesenchymal transition (EMT) is a phenomenon in which epithelial cells lose their cell to cell adhesion and detach from the base of the membrane. EMT is a fundamental process which occurs during tumor progression and metastasis. Cancer genomics is a complex network which involves a variety of factors such as transcription factors (TFs), coding genes and microRNAs (miRs).

Citrinin detection by intensified fluorescence signal of a FRET-based immunosensor using magnetic/silica core-shell.

The specific immune-reaction between the anti-citrinin antibody immobilized on the surface of magnetic/silica core-shell (MSCS) and the citrinin-Rho123-BSA conjugate brings the Rho123 fluorophore as an acceptor and the QDs as a donor in close spatial proximity and causes FRET for occurring upon photo-excitation of the QDs. The novelties of this study include: (1) immobilization of the MSCS; (2) large amount of the immobilized QDs, and (3) immobilization of a large amount of Rho123 on the BSA macromolecule. Cd/Te QDs were synthesized by the simultaneous reduction of cadmium chloride and tellurium in the presence of sodium borohydride.

Evaluation of intrinsic and acquired immune cells infiltration in kidney and spleen of the mice infected with systemic candidiasis and treated with chloroform fraction of Zataria Multiflora Boiss.

Background And Aims: Despite the use of antifungal drugs, the visceral candidiasis is associated with a high mortality rate. The aims of this study were an evaluation of intrinsic and acquired immune cells infiltration in kidney and spleen of the mice infected with systemic candidiasis and treated with chloroform fraction of Zataria Multiflora Boiss.

Materials And Methods: Candida albicans (C.

Effect of long-term exposure of mice to 900 MHz GSM radiation on experimental cutaneous candidiasis.

Mobile phones communicate with base stations using 900 MHz microwaves. The current study was aimed to survey the effects of long-term 900 MHz microwave exposure of mice on experimentally induced cutaneous candidiasis. Forty inbred, male, BALB/c mice were randomly divided into four groups.

Molecular Identification of Mucor and Lichtheimia Species in Pure Cultures of Zygomycetes.

Background: The Mucorales are an important opportunistic fungi that can cause mucormycosis in immunocompromised patients. The fast and precise diagnosis of mucormycosis is very important because, if the diagnosis is not made early enough, dissemination often occurs. It is now well established that molecular methods such as polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) are feasible and reliable tools for the early and accurate diagnosis of mucormycosis agents.

Effect of long-term exposure to mobile phone radiation on alpha-Int1 gene sequence of Candida albicans.

Over the last decade, communication industries have witnessed a tremendous expansion, while, the biological effects of electromagnetic waves have not been fully elucidated. Current study aimed at evaluating the mutagenic effect of long-term exposure to 900-MHz radiation on alpha-Int1 gene sequences of Candida albicans. A standard 900 MHz radiation generator was used for radiation.

Effect of polymer/nanosilver composite packaging on long-term microbiological status of Iranian saffron (Crocus sativus L.).

Crocus sativus L. (saffron) is a valuable plant which is native to Iran. Saffron is the dried stigmata of the flowering part of the plant that is usually contaminated with different bacteria and fungi through production process.

Highly Sensitive FRET-Based Fluorescence Immunoassay for Detecting of Aflatoxin B1 Using Magnetic/Silica Core-Shell as a Signal Intensifier.

Background: Recently, some new nanobiosensors using different nanoparticles or microarray systems for detection of mycotoxins have been designed . However, rapid, sensitive and early detection of aflatoxicosis would be very helpful to distinguish high-risk persons.

Objectives: We report a highly sensitive competitive immunoassay using magnetic/silica core shell as a signal intensifier for the determination of aflatoxin B1 using fluorescence resonance energy transfer (FRET) from Cd/Te quantum dots (antiaflatoxin B1 antibody immobilized on the surface of Cd/Te quantum dots) to Rhodamine 123 (Rho 123-labeled aflatoxin B1 bound to albumin).

Antibacterial Effect of Garlic Aqueous Extract on Staphylococcus aureus in Hamburger.

Background: Using garlic is widespread in Iran and other countries as a medicine and a natural spice. Garlic is a potential inhibitor for food pathogens. Foods contaminated with pathogens pose a potential danger to the consumer's health.

Isolation of Cryptococcus neoformans and other opportunistic fungi from pigeon droppings.

Background: Invasive fungal infections cause considerable morbidity and mortality in immunocompromised hosts. Pigeon droppings could especially be a potential carrier in the spread of pathogenic yeasts and mold fungi into the environment. The objective of this study was to isolation of Cryptococcus neoformans and other opportunistic fungi from pigeon droppings.

Evaluation of The Number of CD4(+) CD25(+) FoxP3(+) Treg Cells in Normal Mice Exposed to AFB1 and Treated with Aged Garlic Extract.

Objective: Aflatoxin B1 (AFB1) suppresses the immune system. To decrease such suppressive effects on the immune system, a wide range of herbal medicines like garlic are utilized. Biological activities of garlic in vitro and in vivo have also been verified.

The effect of Candida albicans systemic infection on matrix metalloproteinases in breast cancer bearing BALB/c mice.

Breast cancer patients are susceptible to infections such as candidiasis. Due to the importance and the role of matrix metalloproteinases (MMP) in breast cancer progression and its correlation with tumor metastasis, we analyzed the serum level of MMPs -2, -3, -9 and tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) in breast cancer bearing mice in the presence of systemic Candida albicans infection. Female BALB/c mice were divided into 4 groups: group I had tumor + candidiasis; group II, tumor only; group III, candidiasis only and group IV as negative control.

Polymorphism in two short tandem repeat loci (R-R and S-Q) linked to tRNA genes in Entamoeba dispar isolates.

Aim: In this study, genetic polymorphism of two tRNA-liked short tandem repeat (STR)-containing loci, R-R and S-Q, was analyzed in order to clarify further the genotypic differences among E. dispar isolates.

Background: Entamoeba dispar is closely related to the human pathogen E.

Multiple subcutaneous cysts due to Exophiala spinifera in an immunocompetent patient.

Here we report a case of a 55-year-old Indian male presenting with multiple subcutaneous cysts, which developed from painful nodules at the dorsal right wrist joint. Subsequently a painful nodule appeared on the left knee joint. Cytological examination of the knee swelling revealed a suppurative inflammatory lesion consisting of neutrophils, lymphocytes, multinucleated giant cells and few fungal elements, without involvement of the overlying skin.