The ratio of the maximum density values: a new method for predicting hemorrhagic transformation in acute ischemic stroke patients undergoing mechanical thrombectomy.

Background: It is challenging yet critical to differentiate between hemorrhagic transformation (HT) and contrast extravasation on non-contrast-enhanced computed tomography (NCCT) scans following mechanical thrombectomy (MT) in patients with acute ischemic stroke. We propose a new method called the ratio of maximum density values (RMDV) to minimize the confusion of contrast extravasation and to evaluate the diagnostic significance of RMDV in predicting HT on immediate post-interventional NCCT scans.

Methods: We conducted a retrospective analysis of the prospective patients' database who received MT for acute ischemic stroke caused by occlusion of the intracranial large artery and showed postinterventional cerebral hyperdensities (PCHDs) on NCCT scans immediately after MT.

Facile synthesis of chlorella-derived autogenous N-doped porous biochar for adsorption on tetracycline.

In this study, an autogenous N-doped biochar derived from Chlorella (CVAC) was prepared with NaOH as activator at 800 °C. The surface structural properties of CVAC and the adsorption performance of CVAC on tetracycline (TC) under different adsorption variables were analyzed and investigated using different characterization methods. The results showed that the specific surface area of CVAC was 491.

The involvement of a novel calmodulin-like protein isoform from oyster Crassostrea gigas in transcription factor regulation provides new insight into acclimation to ocean acidification.

Marine organisms need to adapt to improve organismal fitness under ocean acidification (OA). Recent studies have shown that marine calcifiers can achieve acclimation by stimulating calcium binding/signaling pathways. Here, a CaM-like gene (CgCaLP-2) from oyster Crassostrea gigas which typically responded to long-term CO exposure (two months) rather than short-term exposure (one week) was characterized.

Copyrolysis of microalga Chlorella sp. and alkali lignin with potassium carbonate impregnation for synergistic Bisphenol A plasticizer adsorption.

Composite functional materials offer promising opportunities for the development of tailored adsorbents with enhanced bioremediation potential towards toxic, carcinogenic endocrine disrupters such as Bisphenol A (BPA). Copyrolysis of microalga Chlorella sp. (CH) alkali lignin (L) with KCO impregnation yielded a carbon-based composite (CHL-AC) with a micro-mesoporous structure of 0.

A pattern-free paper enzyme biosensor for one-step detection of fish freshness indicator hypoxanthine with a microfluidic aggregation effect.

In this study, a paper-based enzyme biosensor for hypoxanthine (Hx) was developed, enabling visual and one-step fish freshness detection. Xanthine oxidase and horseradish peroxidase were immobilized on nitrocellulose membranes with 3,3',5,5'-tetramethylbenzidine to output the colour signal. Chitosan oligosaccharide lactate-modified nitrocellulose membranes entrapped the dual-enzyme system and exhibited excellent microfluidic aggregation effect.

Development of highly stable color indicator films based on κ-carrageenan, silver nanoparticle and red grape skin anthocyanin for marine fish freshness assessment.

Color indicator films for fish freshness were fabricated by incorporating κ-carrageenan (CAR) polymer with red grape skin extract (GSE) as a pH-sensing agent and silver nanoparticles (AgNPs) as an antimicrobial agent. Anthocyanins in GSE exhibited distinguished pH responsive color changes. GSE and AgNPs were well compatible with CAR with intramolecular interactions, approved by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) analysis, thermo-gravimetric analysis (TGA) and differential scanning calorimetry (DSC).

Production and waste treatment of polyesters: application of bioresources and biotechniques.

Chemical resources and techniques have long been used in the history of bulk polyester production and still dominate today's chemical industry. The sustainable development of the polyester industry demands more renewable resources and environmentally benign polyester products. Accordingly, the rapid development of biotechnology has enabled the production of an extensive range of aliphatic and aromatic polyesters from renewable bio-feedstocks.

Adsorption modeling, thermodynamics, and DFT simulation of tetracycline onto mesoporous and high-surface-area NaOH-activated macroalgae carbon.

Activated carbon (ENAC) was prepared by NaOH activation, using macroalgae (Enteromorpha clathrate) as raw material. The prepared activated carbon has a large surface area (1238.491 m g) and its total pore volume and average pore size are 0.

Cross-linked FeCl-activated seaweed carbon/MCM-41/alginate hydrogel composite for effective biosorption of bisphenol A plasticizer and basic dye from aqueous solution.

A FeCl-activated seaweed carbon/MCM-41/alginate hydrogel composite (ECAC/MCM-41/ ALG) cross-linked with calcium chloride (2% CaCl) was synthesized for the biosorption of bisphenol A (BPA) plasticizer and basic blue (BB) dye. Biosorption uptakes of BPA and BB were performed in a batch mode with varying solution pH from 3 to 11, initial sorbate concentration from 25 to 300 mg/L, reaction time from 0 to 10 h, and biosorption temperature from 30 to 50 °C. The maximum BPA and BB uptake mechanisms were fast, which occurred within contact times of 1 and 2 h with monolayer coverage capacities of 222.

Matrine Protects Cardiomyocytes Against Hyperglycemic Stress by Promoting Mitofusin 2-Induced Mitochondrial Fusion.

Matrine, an active component of Ait root extracts, has been used in China for years to treat cancer and viral hepatitis. In the present study, we explored the effects of matrine on hyperglycemia-treated cardiomyocytes. Cardiomyocyte function, oxidative stress, cellular viability, and mitochondrial fusion were assessed through immunofluorescence, quantitative real-time PCR (qRT-PCR), enzyme-linked immunosorbent assays, and RNA interference.

Polysaccharide-Based Chiral Stationary Phases on Gold Nanoparticles Modified Silica Beads for Liquid-Phase Separation of Enantiomers.

Au nanoparticles (AuNPs) (10-15 nm in size) were prepared and deposited on the surfaces of silica particles functionalized using 3-aminopropyltriethoxysilane as the seeds under mild conditions. Then, Au seeds grew further and formed nanosheets by the method of gold chloride hydrate reduction. 3, 5-dimethylphenyl isocyanate derivative of cellulose as chiral selector was coated on the surfaces of SiO2/Au.

Development of a direct and visual isothermal method for meat adulteration detection in low resource settings.

Simple, fast and efficient method for on-site detection of meat adulteration is of increasing demand in low-resource regions. Here, a direct and visual denaturation bubble-mediated strand exchange amplification method (SEA) was developed to detect meat adulteration by targeting the mitochondrial sequence from duck, which required only a heating block. The method allowed detecting as low as 10 pg/μL duck DNA and 0.

An ultrasensitive electrochemical DNA sensing strategy free from pre-immobilization via G-quadruplex based homogenous proximity hybridization.

Motivated by the desire for simple, rapid and highly sensitive DNA detection, we presented a signal-on electrochemical DNA (E-DNA) sensing strategy utilizing cooperative proximity hybridization based on a G-quadruplex (G4) probe labeled with the SH, which could specifically hybridize with its target DNA in homogenous solution. In the presence of target DNA, proximity hybridization was triggered to form a Y-shaped complex and the SH was released from G4 probe stem, companied by chemisorption on the electrode surface through Au-S binding when applied a positive potential, which brought Fc labeled on the signal probe close to the electrode surface. Thus, electrochemical signal dramatically increased, ensuring the highly sensitive "signal-on" assay.

Increasing the pyruvate pool by overexpressing phosphoenolpyruvate carboxykinase or triosephosphate isomerase enhances phloroglucinol production in Escherichia coli.

Objectives: Acetyl-CoA is a precursor for phloroglucinol (PG), and pyruvate is one of the sources of intracellular acetyl-CoA. Therefore, enhancing intracellular pyruvate levels may help to improve the anabolic pathway of PG.

Results: In this study, the effects of phosphoenolpyruvate carboxykinase (PckA, encoded by pckA) or triosephosphate isomerase (TpiA, encoded by tpiA) overexpression on the production of PG were studied.

Ultrasensitive electrochemical DNA biosensor based on a tetrahedral structure and proximity-dependent surface hybridization.

The DNA tetrahedron has developed a broad spectrum of applications in biosensor construction thanks to its excellent mechanical rigidity and structural stability. However, how to construct a highly sensitive biosensor using a DNA tetrahedron is still a challenge. In this work, an ultrasensitive electrochemical biosensor based on a DNA tetrahedral nanostructure was developed with the help of synergy from proximity-dependent hybridization.

Lab in a Pasteur pipette: low-cost, rapid and visual detection of Bacillus cereu using denaturation bubble-mediated strand exchange amplification.

Driven by a bright prospect for rapid, portable and cost-effective point-of-care testing, an assembled Pasteur pipette device to integrate nucleic acid extraction, amplification and detection was developed to detect B. cereus in a sample-to-answer format. Denaturation Bubble-mediated Strand Exchange Amplification (SEA) was chosen to perform isothermal amplification because it requires only a pair of primers and one Bst DNA polymerase.

Rapid diagnosis of Mycoplasma pneumonia infection by denaturation bubble-mediated strand exchange amplification: comparison with LAMP and real-time PCR.

M. pneumoniae infection is often ignored due to its similar clinical symptom with respiratory tract infections caused by bacteria or viruses, and thus leading to misdiagnosis and delayed treatment. It is critical to develop a rapid, sensitive and specific diagnosis method.

On-site Method for Beef Detection Based on Strand Exchange Amplification.

Meat screening plays a significant role in human health and religion. But the identification methods for beef were little reported. In this work, a simple colorimetric method based on denaturation bubble-mediated strand exchange amplification (SEA) was developed for the rapid and sensitive identification of beef.

Design, synthesis and biological evaluation of a series of novel 2-benzamide-4-(6-oxy-N-methyl-1-naphthamide)-pyridine derivatives as potent fibroblast growth factor receptor (FGFR) inhibitors.

Starting from the phase II clinical FGFR inhibitor lucitanib (2), we conducted a medicinal chemistry approach by opening the central quinoline skeleton coupled with a scaffold hopping process thus leading to a series of novel 2-benzamide-4-(6-oxy-N-methyl-1-naphthamide)-pyridine derivatives. Compound 25a was identified to show selective and equally high potency against FGFR1/2 and VEGFR2 with IC values less than 5.0 nM.

SOMCL-085, a novel multi-targeted FGFR inhibitor, displays potent anticancer activity in FGFR-addicted human cancer models.

Aberrant fibroblast growth factor receptor (FGFR) activation is found across a diverse spectrum of malignancies, especially those lacking effective treatments. SOMCL-085 is a novel FGFR-dominant multi-target kinase inhibitor. Here, we explored the FGFR-targeting anticancer activity of SOMCL-085 both in vitro and in vivo.

SAR study of 5-alkynyl substituted quinazolin-4(3H)-ones as phosphoinositide 3-kinase delta (PI3Kδ) inhibitors.

PI3Kδ is a key component in the aberrant signaling transduction in B cell malignancy, therefore specific targeting PI3Kδ has become an attractive molecularly targeted therapy for chronic lymphocytic leukemia (CLL). Herein, we describe the discovery and optimization of a series of 5-alkynyl substituted PI3Kδ inhibitors based on the first FDA-approved inhibitor idelalisib. Compound 8d bearing the 1-morpholinohex-5-yn-1-one moiety as the C5-substituent was identified to have high potency against PI3Kδ (3.

Self-assembled cyclodextrin-modified gold nanoparticles on silica beads as stationary phase for chiral liquid chromatography and hydrophilic interaction chromatography.

A facile strategy based on self-assembly of Au nanoparticles (AuNPs) (60±10nm in size) on the surfaces of amino-functionalized porous silica spheres under mild conditions was proposed. The resulting material possessed a core-shell structure in which AuNPs were the shell and silica spheres were the core. Then, thiolated-β-cyclodextrin (SH-β-CD) was covalently attached onto the AuNPs as chiral selector for the enantioseparation.

Enhancement and monitoring of pollutant removal in a constructed wetland by microbial electrochemical technology.

A bench-scale constructed wetland combined with microbial electrochemical technology (MET-CW) was run for 400days with groundwater contaminated with benzene, methyl-tert-butyl ether (MTBE), and ammonium (NH4(+)). Four vertically stacked anode modules were embedded into a sand bed and connected with a stainless steel cathode placed in an open water pond. In the zone of presence of anode modules, significantly more benzene and MTBE were removed in the MET-CW compared to the control CW without MET in the first 150 operation days.

Targeting PI3Kδ: emerging therapy for chronic lymphocytic leukemia and beyond.

Chronic lymphocytic leukemia (CLL) remains the most incurable leukemia. Early chemotherapeutic treatments, including alkylating agents, purine nucleoside derivatives, and immunotherapeutic antibodies, only show limited benefits for patients but severe off-target related side effects. Recent advances in understanding of the critical molecular pathways of regulating proliferation and survival of B-CLL cells have spurred a new therapeutical strategy by selectively targeting phosphoinositide 3-kinase delta (PI3Kδ).

Application of real-time PCR to monitor population dynamics of defined mixed cultures of moderate thermophiles involved in bioleaching of chalcopyrite.

To compare oxidative dissolution rates of chalcopyrite by different consortia of moderately thermophilic acidophiles, various defined mixed cultures of three bacteria Acidithiobacillus caldus s2, Leptospirillum ferriphilum YSK, and Sulfobacillus sp. LN and one archaeon Ferroplasma thermophilum L1 were studied in batch shake flask cultures incubated at 45 degrees C. Chalcopyrite dissolution was determined by measuring variations of soluble copper, ferric iron, and pH.