A novel fluorescence probe for simultaneous detection of mitochondrial viscosity in hepatic ischemia reperfusion injury models.

Acute liver failure caused by hepatic ischemia reperfusion injury (HIRI) poses a severe threat to life, emphasizing the urgent need for precise and timely early diagnosis. Viscosity, a key parameter reflecting active analyte levels at the cellular level, remains underexplored in relation to HIRI. To address this gap, we have developed a groundbreaking near-infrared molecule rotator, PN, exhibiting exceptional characteristics.

Development of a novel near-infrared molecule rotator for early diagnosis and visualization of viscosity changes in acute liver injury models.

Acute liver injury leading to acute liver failure can be a life-threatening condition. Therefore, timely and accurate early diagnosis of the onset of acute liver injury is critical. Viscosity is one of the key parameters that can accurately reflect the levels of relevant active analytes at the cellular level.

Modular synthesis of clickable peptides via late-stage maleimidation on C(7)-H tryptophan.

Cyclic peptides have attracted tremendous attention in the pharmaceutical industry owing to their excellent cell penetrability, stability, thermostability, and drug-like properties. However, the currently available facile methodologies for creating such peptides are rather limited. Herein, we report an efficient and direct peptide cyclization via rhodium(III)-catalyzed C(7)-H maleimidation.

Ultrasensitive Small-Molecule Fluorescent Thermometer Reveals Hot Mitochondria in Surgically Resected Human Tumors.

The Warburg effect suggests that upregulated glycolysis arising from high glucose uptake in cancer cells might be accompanied with suppressed mitochondrial respiration. However, recent studies have shown that the mitochondrial temperature in cancer cells could be relatively higher than that in normal cells, suggesting hyperactive mitochondrial respiration in cancer cells. However, hot mitochondria have not been reported in patients with cancer.

Recent progress on small molecular temperature-sensitive fluorescent probes.

Temperature is an important biophysical parameter that is closely related with the metabolic activity in living cells. Therefore, the detection of intracellular temperature changes is crucial for exploring temperature-related biological processes. Fluorescence probe is an ideal tool for observing temperature changes in cells, which has many advantages, such as high sensitivity, good selectivity, and noninvasive, and thus aroused the great interest of researchers.

A dual-response fluorescent probe for NH and viscosity in living cells and zebrafish to evaluate liver injury.

Hydrazide drugs can cause severe drug-induced liver injury owing to the enzymatic release of NH in the liver. Also, changes in cellular viscosity are associated with liver damage. Thus, simultaneous monitoring of changes in NH levels and viscosity can be used to evaluate the side effects of hydrazide drugs.

A novel dual-functional fluorescent probe for imaging viscosity and cysteine in living system.

Abnormal changes in intracellular viscosity and cysteine are both associated with several important biological processes such as reversible redox reactions, which play a pivotal role in the process of inflammation. However, it remains unclear how cysteine and viscosity are altered in inflammation. Herein, we firstly report a high-sensitivity and -selectivity near-infrared imaging probe (FCV) for tracking intracellular viscosity and endogenous cysteine.

A bifunctional probe reveals increased viscosity and hydrogen sulfide in zebra fish model of Parkinson's disease.

Altered HS levels and intracellular viscosity have both been seen in Parkinson's disease (PD). However, how HS and intracellular viscosity are involved in PD pathogenesis remains unknown. Herein, a dual-function fluorescent probe DF was designed and synthesized to analyze intracellular viscosity and hydrogen sulfide.

A 1,8-naphthalimide-based lysosome-targeting dual-analyte fluorescent probe for the detection of pH and palladium in biological samples.

Fluorescent probes containing 1,8-naphthalimide dyes have been used to detect biomolecules in the environmental and biological fields. However, most of the probes only exhibit single fluorescent output to one analyte, making them insufficient for detection of more analytes. Herein, we developed a novel 1,8-naphthalimide-based lysosome-targeting dual-analyte sensitive fluorescent probe (DPPP) for the detection of pH and palladium (Pd) using two different emissive channels.

A dual-rotator fluorescent probe for analyzing the viscosity of mitochondria and blood.

A novel mitochondria-targeting molecular rotator FD was designed to visualize changes in viscosity under hypoxic conditions in vitro and in vivo. Importantly, FD can be used to detect changes in the blood viscosity of liver cancer and liver cirrhosis patients, and also rehabilitation of liver disease.

Dual ligand-promoted palladium-catalyzed nondirected C-H alkenylation of aryl ethers.

Direct C-H functionalization of aryl ethers remains challenging owing to their low reactivity and selectivity. Herein, a novel strategy for nondirected C-H alkenylation of aryl ethers promoted by a dual ligand catalyst was demonstrated. This catalytic system readily achieved the highly efficient alkenylation of alkyl aryl ethers (anisole, phenetole, n-propyl phenyl ether, n-butyl phenyl ether and benzyl phenyl ether), cyclic aryl ethers (1,4-benzodioxan, 2,3-dihydrobenzofuran, dibenzofuran), and diphenyl oxides.

A highly sensitive red-emitting probe for the detection of viscosity changes in living cells, zebrafish, and human blood samples.

Intracellular viscosity can be measured to reflect the state of living cells. Fluorescent probes are powerful tools for viscosity detection in vivo. Herein, we report on a novel red-emitting viscosity-sensitive probe DJH with a large Stokes shift of 165 nm, showing a 400-fold fluorescence enhancement from PBS solution to 90% glycerol.