Recent advances in metal-based nanomaterials for malignant bone tumor therapy.

Malignant bone tumors seriously affect the quality of life of terminal patients and impose severe economic burdens on social health management owing to the complex pathogenesis, multiresistance, tumor recurrence, and metastasis, for which traditional clinical treatments are difficult to achieve the ideal therapeutic effects. Metal-based nanomaterials have shown great application potential in the treatment of malignant bone tumors because of their unique physicochemical properties, biological activities, and structural features, which have greatly improved their ability to kill malignant bone tumor cells and inhibit bone tumor growth by being designed as carriers, therapeutic agents, and coatings/scaffolds, combined with multimodal treatment methods, which have effectively overcome the problems of low efficacy, easy resistance, metastasis, and recurrence faced by traditional treatment methods. This paper summarizes the latest progress in malignant bone tumor treatment using metal-based nanomaterials through three modes of carriers, therapeutic agents, and coatings/scaffolds in recent years.

Ratiometric fluorescence analysis of tetracycline by dual-ligand europium-based metal-organic framework.

It is important to develop sensitive and accurate methods for the detection of tetracycline (TC). For this purpose, a dual emission probe Eu-DPA/BDC-(OH) was developed based on the green fluorescence of the ligand 2, 5-dihydroxyterephthalic acid (BDC-(OH)), and the red fluorescence of Eu sensitized by another ligand 2, 6-pyridine dicarboxylic acid (DPA). TC was able to quench the fluorescence of ligand BDC-(OH) at 545 nm by the internal filter effect (IFE), and coordinate with Eu to enhance the fluorescence at 625 nm by the antenna effect (AE).

Construction of Zinc-based metal-organic frameworks for visual ratiometric fluorescence detection of tetracycline.

Background: Tetracycline (TC) is frequently utilized as additives in animal feed. The misuse can lead to the residue in food and threaten human health.

Results: A ratiometric fluorescence method for tetracycline detection is developed based on Zinc-based metal-organic framework (Zn-MOF) materials.

Ratiometric fluorescence probe based on boric acid-modified carbon dots and alizarin red for sensitive and rapid detection of glyphosate.

By combining boric acid-modified carbon dots (p-CDs) and alizarin red (ARS), a double emission probe p-CDs@ARS with fluorescence at 410 nm and 600 nm is designed for the detection of glyphosate. When Cu is added, it binds with ARS to cause ARS release from p-CDs@ARS, which decreases the fluorescence at 600 nm. However, in the presence of glyphosate, glyphosate competes to the binding of Cu, releasing ARS to bind with p-CDs again.

Prospects, advances and biological applications of MOF-based platform for the treatment of lung cancer.

Nowadays in our society, lung cancer is exhibiting a high mortality rate and threat to human health. Conventional diagnostic techniques used in the field of lung cancer often necessitate the use of extensive instrumentation, exhibit a tendency for false positives, and are not suitable for widespread early screening purposes. Conventional approaches to treat lung cancer primarily involve surgery, chemotherapy, and radiotherapy.

MOF-Based Platform for Kidney Diseases: Advances, Challenges, and Prospects.

Kidney diseases are important diseases that affect human health worldwide. According to the 2020 World Health Organization (WHO) report, kidney diseases have become the top 10 causes of death. Strengthening the prevention, primary diagnosis, and action of kidney-related diseases is of great significance in maintaining human health and improving the quality of life.

Ethanolic extract from Sophora moorcroftiana inhibit cell proliferation and alter the mechanical properties of human cervical cancer.

Background: Cervical cancer is one of the most common gynecological malignancies. Previous studies have shown that the ethanol extract of Sophora moorcroftiana seeds (EESMS) possesses an antiproliferative effect on several tumors in vitro. Therefore, in this study, we assessed the impact of EESMS on human cervical carcinoma (HeLa) cell proliferation.

Self-assembly of hyperbranched DNA network structure for signal amplification detection of miRNA.

Catalytic hairpin assembly (CHA) and hybridization chain reaction (HCR) can achieve the high sensitivity and rapid reaction rate in detecting miRNA. However, the amplification efficiency by these methods are limited. Herein, an enzyme-free and label-free hyperbranched DNA network structure (HDNS) was designed, in which localized catalytic hairpin assembly (LCHA) and hybridization chain reaction occurred in the horizontal axis and longitudinal axis, respectively, exhibiting intensive signal dual-amplification.

Phosphorus Modulated Peroxidase-Like Activity of Carbon Dots for Colorimetric Detection of Acid Phosphatase.

The precise regulation of nanoenzyme activity is of great significance for application to biosensing analysis. Herein, the peroxidase-like activity of carbon dots was effectively modulated by doping phosphorus, which was successfully employed for sensitive, selective detection of acid phosphatase (ACP). Phosphorus-doped carbon dots (P-CDs) with excellent peroxidase-like activity were synthesized by a one-pot hydrothermal method, and the catalytic activity could be easily modulated by controlling the additional amount of precursor phytic acid.

SOCS1 as a Biomarker Candidate for HPV Infection and Prognosis of Head and Neck Squamous Cell Carcinomas.

The pathogenesis of head and neck squamous cell carcinoma (HNSCC) is associated with human papillomavirus (HPV) infection. However, the molecular mechanisms underlying the interactions between HNSCC and HPV remain unclear. Bioinformatics was used to analyze the gene expression dataset of HPV-associated HNSCC based on the Cancer Genome Atlas (TCGA) database.

Association between anemia and the risk and outcomes of diabetic foot in patients with type 2 diabetes mellitus.

The aim of the present study was to explore the association between anemia and the risks and outcomes of diabetic foot (DF) in patients with type 2 diabetes mellitus (T2DM). A total of 145 patients with T2DM were recruited between January and December 2021 and divided into the DF and non-DF groups according to whether they were diagnosed with DF. Individual patient data were extracted and blood samples were evaluated in a biochemical center for routine biochemical and blood-related indicators.

Enzyme activity termination by titanium carbide nanosheet and its application for the detection of deoxyribonuclease I.

Deoxyribonuclease I (DNase I) is a typical nuclease that plays key roles in many physiological processes and the development of a novel biosensing strategy for DNase I detection is of fundamental significance. In this study, a fluorescence biosensing nanoplatform based on a two-dimensional (2D) titanium carbide (TiC) nanosheet for sensitive and specific detection of DNase I was reported. Fluorophore-labeled single-stranded DNA (ssDNA) can be spontaneously and selectively adsorbed on TiC nanosheet through the hydrogen bond and metal chelate interaction between phosphate groups of ssDNA and titanium of TiC nanosheet, resulting in effective quenching of the fluorescence emitted by fluorophore.

An electrochemical determination strategy for miRNA based on bimetallic nanozyme and toehold-mediated DNA replacement procedure.

An electrochemical strategy based on bimetallic nanozyme in collaboration with toehold-mediated DNA replacement effect is proposed for the sensitive determination of miRNA-21. The AuPt nanoparticles (AuPt NPs) are prepared as a catalytic beacon; it shows favorable peroxidase properties with a Michaelis contant (K) of 0.072 mM for HO, which is capable of catalyzing HO to induce an intense redox reaction, and causing a measurable electrochemical signal.

Cu-mediated turn-on fluorescence biosensor based on DNA-templated silver nanoclusters for label-free and sensitive detection of adenosine triphosphate.

A Cu-mediated turn-on fluorescence biosensor based on the DNA-templated green-emitting silver nanoclusters (DNA@g-AgNCs) was developed for label-free and sensitive detection of adenosine 5'-triphosphate (ATP). Cu was able to quench the bright green fluorescence of DNA@g-AgNCs because of the coordination and photoinduced electron transfer between DNA@g-AgNCs and Cu. Therefore, a unique and effective fluorescence biosensor can be constructed with the formation of DNA@g-AgNCs/Cu/ATP ternary-competition system.

Real-world effect of the treatment for painful subacute thyroiditis: The combined injection of lidocaine and triamcinolone using an insulin pen.

Objective: : Intrathyroidal injection using an insulin pen filled with a mixture of lidocaine and triamcinolone acetonide is a therapy for subacute thyroiditis (SAT) reported by us previously. We aimed to evaluate the clinical efficacy of ultrasound-guided intrathyroidal injection in the treatment of SAT.

Subjects And Methods: A total of 93 patients with SAT completed the study.

Nitrogen-doped magnetic porous carbon material from low-cost anion-exchange resin as an efficient adsorbent for tetracyclines in water.

In this work, nitrogen-doped magnetic porous carbon material (N-MPC) was prepared through the high-temperature calcination of low-cost [Fe(CN)]-loaded anion-exchange resin, which was experimentally demonstrated to have significant adsorption performance for tetracycline (TC) in water. The N-MPC adsorbent with a large specific surface area (781.1 m g) was able to maintain excellent performance in a wide pH range from 4 to 10 or in high ionic strength solution.

A Facile, Label-free and Versatile Fluorescence Sensing Nanoplatform Based on Titanium Carbide Nanosheets for the Detection of Various Targets.

The construction of a universal nanoplatform for sensitive detection of multiple targets of interest is of great importance in different research fields. Herein, by ingeniously integrating the target recognition sequences and G-rich sequences into a single-stranded multifunctional DNA probe and adopting TiC nanosheets as an efficient fluorescence quencher, a simple, low-cost and easy operation fluorescence sensing nanoplatform was proposed. Without an analytical target, the hydrogen bond and metal chelate interaction between the target recognition region of the DNA probe and TiC nanosheet induce the selective self-assembly of highly fluorescent thioflavin T (ThT)-intercalated DNA probe onto the surface of TiC nanosheets, resulting in dramatic decrease of fluorescence emitted by ThT-G-quadruplex.

Differences in Supragingival Microbiome in Patients with and without Full-Crown Prostheses.

To characterize the microflora profile of supragingival biofilm in patients with and without full-crown prostheses. Plaque samples of full-crown prostheses and teeth in patients with porcelain-fused-to-metal crowns, all-ceramic crowns, and no prostheses were collected (three patients per group), using 16S rRNA high-throughput sequencing technology to conduct DNA sequencing on the samples and using Qiime, R, and PICRUSt2 software to perform bioinformatics analyses and functional analyses on sequencing data. In total, 110,209 valid sequences were obtained in the experiment, corresponding to 11 phyla and 120 genera.

Platelet-derived microRNA-223 attenuates TNF-α induced monocytes adhesion to arterial endothelium by targeting ICAM-1 in Kawasaki disease.

Background: Kawasaki disease (KD) is an acute vasculitis that may result in permanent coronary artery damage with unknown etiology. Endothelial cell (EC) dysfunction and platelet hyperactivity are the hallmarks of KD. Platelets are involved in the development of endothelial dysfunction.

Gasdermin D-dependent platelet pyroptosis exacerbates NET formation and inflammation in severe sepsis.

Platelets have emerged as key inflammatory cells implicated in the pathology of sepsis, but their contributions to rapid clinical deterioration and dysregulated inflammation have not been defined. Here, we show that the incidence of thrombocytopathy and inflammatory cytokine release was significantly increased in patients with severe sepsis. Platelet proteomic analysis revealed significant upregulation of gasdermin D (GSDMD).

Redox-Responsive Breakup of a Nucleic Acids@CoOOH Nanocomplex Triggering Cascade Recycling Amplification for Sensitive Sensing of Alkaline Phosphatase.

Alkaline phosphatase (ALP), an essential hydrolase with crucial roles in living organisms, has widely been regarded as a biomarker for various human diseases in clinical diagnoses. Herein, taking advantage of cobalt oxyhydroxide (CoOOH) nanoflakes and nonenzymatic cascade recycling amplification (CRA), a highly sensitive and label-free fluorescence biosensing strategy for the determination of ALP activity is introduced. In our design, ALP can promote the dephosphorylation of l-ascorbic acid 2-phosphate (AAP) to reduce ascorbic acid (AA), which is then able to decompose CoOOH in a nucleic acids@CoOOH nanocomplex into Co cofactors.

A ratiometric fluorescence strategy based on inner filter effect for Cu-mediated detection of acetylcholinesterase.

A novel ratiometric fluorescence strategy for detection of acetylcholestinerase (AChE) is proposed based on carbon nitride quantum dots (g-CNQD) and the complex (PA) formed between phenylboronic acid (PBA) and alizarin red S (ARS). PA showed fluorescence at 598 nm and quenched the fluorescence of g-CNQD at 438 nm. Through UV-visible absorption, fluorescence, and fluorescence lifetime measurements, the quenching effect was demonstrated as inner filter effect (IFE).

Novel mutations of the gene in patients with Gitelman syndrome.

Mutations in the SLC12A3 gene have been reported to cause Gitelman syndrome (GS). This study aimed to investigate the genetic mutations and clinical features of patients with GS. Four pedigrees (4 GS patients and 14 family members) were enrolled.