Revolutionizing DNA Extraction: A Cost-Effective Approach for Genomic DNA Retrieval from Dried Blood Spots.

Background: This study introduces an efficient, cost-effective laboratory- derived method for extracting genomic DNA from dried blood spots (DBS) by optimizing the organic separation phenol method.

Methodology: DBS samples, collected via heel prick from 50 neonates as a part of routine newborn screening, were processed using an optimized phenol method that employs lysis buffers with minimal concentrations of proteinase K and phenol:chloroform:isoamyl alcohol (PCI) reagent.

Results: The extracted genomic DNA exhibited a concentration range of 50 to 200ng/μl, with purity levels (A260/280) falling within the range of 1.