A qPCR Assay for the Detection of Including an mRNA Protocol Designed to Establish Propagule Viability in Environmental Samples.

causes root and collar rot in many plant species in natural ecosystems and horticulture. A species-specific primer and probe PCIN5 were designed based on a mitochondrial locus encoding subunit 2 of cytochrome c oxidase (2). Eight PCR primers, including three forward and five reverse, were designed and tested in all possible combinations.