Acetone as a greener alternative to acetonitrile in liquid chromatographic fingerprinting.

A considerable amount of chemical waste from liquid chromatography analysis is generated worldwide. Acetonitrile is the most employed solvent in liquid chromatography analyses since it exhibits favorable physicochemical properties for separation and detection, but it is an unwelcome solvent from an environmental point of view. Acetone might be a much greener alternative to replace acetonitrile in reversed-phase liquid chromatography, since both share similar physicochemical properties, but its applicability with ultraviolet absorbance-based detectors is limited.

Assessment of the complementarity of temperature and flow-rate for response normalisation of aerosol-based detectors.

The solvent dependency of the detection response is a major limitation of corona-charged aerosol detection (C-CAD). The present study empirically investigates the utility of temperature and flow-rate gradients to overcome solvent gradient limitations of C-CAD. In preliminary flow-injection investigations, it is demonstrated that the response of C-CAD remains relatively unaltered with variations in flow-rate when used with water-rich eluents.

Effects of eluent temperature and elution bandwidth on detection response for aerosol-based detectors.

Aerosol detectors provide generally uniform response for most analytes, independent of their optical properties, and have the advantage of being compatible with elevated temperature mobile phases. Therefore, aerosol detectors present an attractive detection alternative for high temperature liquid chromatography (HTLC) separations. The present study has investigated the effects of HTLC conditions using aqueous mobile phases on the detection response of an evaporative light-scattering detector (ELSD) and a corona-charged aerosol detector (C-CAD).