Effect of aging on the toxicity of polyethylene microplastics on the estuarine bivalve Scrobicularia plana.

Microplastics (MP) are now present in all ecosystems and undergo weathering processes, including physical or chemical degradation. Although most studies have been carried out on MP toxicity in the marine ecosystem, interest is growing for the terrestrial and entire aquatic compartments. However, the interface between both environments, also known as the soil/water continuum, is given little consideration in MP toxicity studies.

A template wizard for the cocreation of machine-readable data-reporting to harmonize the evaluation of (nano)materials.

Making research data findable, accessible, interoperable and reusable (FAIR) is typically hampered by a lack of skills in technical aspects of data management by data generators and a lack of resources. We developed a Template Wizard for researchers to easily create templates suitable for consistently capturing data and metadata from their experiments. The templates are easy to use and enable the compilation of machine-readable metadata to accompany data generation and align them to existing community standards and databases, such as eNanoMapper, streamlining the adoption of the FAIR principles.

Safe(r) by design guidelines for the nanotechnology industry.

Expectations for safer and sustainable chemicals and products are growing to comply with the United Nations and European strategies for sustainability. The application of Safe(r) by Design (SbD) in nanotechnology implies an iterative process where functionality, human health and safety, environmental and economic impact and cost are assessed and balanced as early as possible in the innovation process and updated at each step. The EU H2020 NanoReg2 project was the first European project to implement SbD in six companies handling and/or manufacturing nanomaterials (NMs) and nano-enabled products (NEP).

Integrative approach in a safe by design context combining risk, life cycle and socio-economic assessment for safer and sustainable nanomaterials.

Moving towards safe and sustainable innovations is an international policy ambition. In the on-hand manuscript, a concept combining safe by design and sustainability was implemented through the integration of human and environmental risk assessment, life cycle assessment as well as an assessment of the economic viability. The result is a nested and iterative process in form of a decision tree that integrates these three elements in order to achieve sustainable, safe and competitive materials, products or services.

Nanopharmaceuticals (Au-NPs) after use: Experiences with a complex higher tier test design simulating environmental fate and effect.

The current environmental hazard assessment is based on the testing of the pristine substance. However, it cannot be excluded that (nano)pharmaceuticals are excreted into sewage during the use phase followed by entry into wastewater treatment plants (WWTPs). Sorption to sewage sludge or release via effluent can result in modified ecotoxicological effects which possibly can only be detected with a modified test approach.

Comparison of uptake and elimination kinetics of metallic oxide nanomaterials on the freshwater microcrustacean .

The widespread use and release of nanomaterials (NMs) in aquatic ecosystems is a concerning issue as well as the fate and behavior of the NMs in relation to the aquatic organisms. In this work, the freshwater microcrustacean was exposed to 12 different and well-known NMs under the same conditions for 24 h and then placed in clean media for 120 h, in order to determine their different uptake and elimination behaviors. The results showed that most of the tested NMs displayed a fast uptake during the first hours arriving to a plateau by the end of the uptake phase.

Towards FAIR nanosafety data.

Nanotechnology is a key enabling technology with billions of euros in global investment from public funding, which include large collaborative projects that have investigated environmental and health safety aspects of nanomaterials, but the reuse of accumulated data is clearly lagging behind. Here we summarize challenges and provide recommendations for the efficient reuse of nanosafety data, in line with the recently established FAIR (findable, accessible, interoperable and reusable) guiding principles. We describe the FAIR-aligned Nanosafety Data Interface, with an aggregated findability, accessibility and interoperability across physicochemical, bio-nano interaction, human toxicity, omics, ecotoxicological and exposure data.

Plastic additives: challenges in ecotox hazard assessment.

The risk of plastic debris, and specifically micro(nano)plastic particles, to ecosystems remains to be fully characterized. One particular issue that warrants further characterization is the hazards associated with chemical additives within micro(nano)plastic as they are not chemically bound within the polymers and can be persistent and biologically active. Most plastics contain additives and are therefore potential vectors for the introduction of these chemicals into the environment as they leach from plastic, a process that can be accelerated through degradation and weathering processes.

Characterization of the nano-bio interaction between metallic oxide nanomaterials and freshwater microalgae using flow cytometry.

Since nanomaterials (NMs) are particulate contaminants, their first contact with organisms is a physical encounter ruled by physic-chemical processes that can determinate the potential NMs accumulation, toxicity, and trophic transfer. Freshwater ecosystems often become a final depository for NMs, so they can get in contact with the biota, especially primary organisms as algae. There are almost none comparative studies of this interaction using various NMs in the same conditions.

Investigating the Impact of Manufacturing Processes on the Ecotoxicity of Carbon Nanofibers: A Multi-Aquatic Species Comparison.

Manufactured nanomaterial production is outpacing the ability to investigate environmental hazard using current regulatory paradigms, causing a backlog of materials requiring testing. To ameliorate this issue, regulatory bodies have proposed integrating safety into the production of novel nanomaterials, allowing for hazards to be identified early in development rather than aftermarket release. In addition, there is a growing interest in short-term ecotoxicity testing to rapidly identify environmental hazards.

Investigating a transcriptomic approach on marine mussel hemocytes exposed to carbon nanofibers: An in vitro/in vivo comparison.

Manufactured nanomaterials are an ideal test case of the precautionary principle due to their novelty and potential environmental release. In the context of regulation, it is difficult to implement for manufactured nanomaterials as current testing paradigms identify risk late into the production process, slowing down innovation and increasing costs. One proposed concept, namely safe(r)-by-design, is to incorporate risk and hazard assessment into the design process of novel manufactured nanomaterials by identifying risks early.

Towards the development of a high throughput screening approach for Mytilus edulis hemocytes: A case study on silicon-based nanomaterials.

To have an understanding of potential mechanistic effects, sublethal endpoints able to discriminate between nanomaterials with similar physical and chemical features need to be used. In this sense, quantitative PCR was used to measure a battery of genes linked to a wide array of different cellular processes. Gene expression was measured in Mytilus edulis hemocytes following an in vitro and in vivo exposure to pure silicon (40 nm) and carbon-coated silicon (40 and 75 nm) after 24 h.

Evaluation of the extraction efficiency for the Hypoaspis aculeifer reproduction test in the context of soil quality assessment.

The standardized bioassay using the predatory mite Hypoaspis aculeifer (TG 226; as reported by OECD 2008) has already proven its usefulness for the assessment of chemicals. While included in the regulatory assessment scheme of pesticides as a non-target arthropod, it has still been rarely used for the assessment of soils or complex matrices of unknown quality. For such an objective, the extraction of both adults and juveniles from soil is a necessary and crucial step to get reliable data, but may be influenced by the characteristics of the tested soils or matrices.

Environmental release of engineered nanomaterials from commercial tiles under standardized abrasion conditions.

The study presented here focuses on commercial antibacterial tiles whose emissivity of (nano) particles due to abrasion has yet barely been investigated. The tiles have been characterized regarding their surface properties and composition throughout their chain-of-use, i.e.

The comet assay in higher terrestrial plant model: Review and evolutionary trends.

The comet assay is a sensitive technique for the measurement of DNA damage in individual cells. Although it has been primarily applied to animal cells, its adaptation to higher plant tissues significantly extends the utility of plants for environmental genotoxicity research. The present review focuses on 101 key publications and discusses protocols and evolutionary trends specific to higher plants.

Adapting OECD Aquatic Toxicity Tests for Use with Manufactured Nanomaterials: Key Issues and Consensus Recommendations.

The unique or enhanced properties of manufactured nanomaterials (MNs) suggest that their use in nanoenabled products will continue to increase. This will result in increased potential for human and environmental exposure to MNs during manufacturing, use, and disposal of nanoenabled products. Scientifically based risk assessment for MNs necessitates the development of reproducible, standardized hazard testing methods such as those provided by the Organisation of Economic Cooperation and Development (OECD).

Ecotoxicological assessment of organic wastes spread on land: Towards a proposal of a suitable test battery.

The land spreading of organic wastes in agriculture is a common practice in Europe, under the regulation of the Directive 86/278/EEC. One of the objectives of this Directive is to prevent harmful effects of organic wastes on soil, plants and animals. Despite this regulatory framework, there is still a lack of harmonized ecotoxicological test strategy to assess the environmental hazard of such wastes.

The use of soil mites in ecotoxicology: a review.

Mites, and especially soil-inhabiting ones, have been less studied than the other invertebrates used in bio-assays for the assessment of soil quality and the hazards of chemicals, although these organisms are included in the regulatory assessment scheme of pesticides. The recent advances in the development of test methods for soil mites groups have provided more information on their sensitivities towards chemicals, which needs to be presented for a more robust assessment of the current trends in soil mite ecotoxicology. Moreover, interestingly mite is the only taxa for which test methods were developed and standardized on predatory organisms.

Soil genotoxicity assessment--results of an interlaboratory study on the Vicia micronucleus assay in the context of ISO standardization.

The Vicia micronucleus assay was standardized in an international protocol, ISO 29200, "Assessment of genotoxic effects on higher plants-Vicia faba micronucleus test," for soil or soil materials (e.g., compost, sludge, sediment, waste, and fertilizing materials).

Selecting chemical and ecotoxicological test batteries for risk assessment of trace element-contaminated soils (phyto)managed by gentle remediation options (GRO).

During the past decades a number of field trials with gentle remediation options (GRO) have been established on trace element (TE) contaminated sites throughout Europe. Each research group selects different methods to assess the remediation success making it difficult to compare efficacy between various sites and treatments. This study aimed at selecting a minimum risk assessment battery combining chemical and ecotoxicological assays for assessing and comparing the effectiveness of GRO implemented in seven European case studies.

Ecotoxicity of non-aged and aged CeO2 nanomaterials towards freshwater microalgae.

The ecotoxicity of artificially alterated cerium dioxide nanoparticles (nano-CeO2) suspensions was determined using the freshwater microalgae growth inhibition test. The agglomeration or aggregation state of the alterated suspensions was followed because it represents one of the obvious modifications when nanoparticles reached the environment. In addition, its influence on the ecotoxicity of nanoparticles is currently not well-addressed.

Improvement of the Caenorhabditis elegans growth and reproduction test to assess the ecotoxicity of soils and complex matrices.

A growth and reproduction test using the nematode Caenorhabditis elegans was recently standardized by the International Organization for Standardization (ISO). Performing the ISO 10872 protocol (2010) revealed some drawbacks when applied to soil or soil mixed with complex matrices. The authors propose some modifications to the current protocol to normalize the test conditions.

White clover nodulation index in heavy metal contaminated soils- a potential bioindicator.

The morphological effects of heavy metal stress on the nodulation ability of Rhizobium spp. and growth of white clover (Trifolium repens L.) were studied in the laboratory under controlled conditions.