Roadmap on computational methods in optical imaging and holography [invited].

Unlabelled: Computational methods have been established as cornerstones in optical imaging and holography in recent years. Every year, the dependence of optical imaging and holography on computational methods is increasing significantly to the extent that optical methods and components are being completely and efficiently replaced with computational methods at low cost. This roadmap reviews the current scenario in four major areas namely incoherent digital holography, quantitative phase imaging, imaging through scattering layers, and super-resolution imaging.

Intelligent Beam Optimization for Light-Sheet Fluorescence Microscopy through Deep Learning.

Light-sheet fluorescence microscopy (LSFM) provides the benefit of optical sectioning coupled with rapid acquisition times, enabling high-resolution 3-dimensional imaging of large tissue-cleared samples. Inherent to LSFM, the quality of the imaging heavily relies on the characteristics of the illumination beam, which only illuminates a thin section of the sample. Therefore, substantial efforts are dedicated to identifying slender, nondiffracting beam profiles that yield uniform and high-contrast images.

Enhancing Light-Sheet Fluorescence Microscopy Illumination Beams through Deep Design Optimization.

Light sheet fluorescence microscopy (LSFM) provides the benefit of optical sectioning coupled with rapid acquisition times for imaging of tissue-cleared specimen. This allows for high-resolution 3D imaging of large tissue volumes. Inherently to LSFM, the quality of the imaging heavily relies on the characteristics of the illumination beam, with the notion that the illumination beam only illuminates a thin section that is being imaged.

Deep learning-based adaptive optics for light sheet fluorescence microscopy.

Light sheet fluorescence microscopy (LSFM) is a high-speed imaging technique that is often used to image intact tissue-cleared specimens with cellular or subcellular resolution. Like other optical imaging systems, LSFM suffers from sample-induced optical aberrations that decrement imaging quality. Optical aberrations become more severe when imaging a few millimeters deep into tissue-cleared specimens, complicating subsequent analyses.

Maternal organophosphate flame retardant exposure alters the developing mesencephalic dopamine system in fetal rat.

Organophosphate flame retardants (OPFRs) have become the predominant substitution for legacy brominated flame retardants but there is concern about their potential developmental neurotoxicity (DNT). OPFRs readily dissociate from the fireproofed substrate to the environment, and they (or their metabolites) have been detected in diverse matrices including air, water, soil, and biota, including human urine and breastmilk. Given this ubiquitous contamination, it becomes increasingly important to understand the potential effects of OPFRs on the developing nervous system.

Quantitative analysis of illumination and detection corrections in adaptive light sheet fluorescence microscopy.

Light-sheet fluorescence microscopy (LSFM) is a high-speed, high-resolution and minimally phototoxic technique for 3D imaging of in vivo and in vitro specimens. LSFM exhibits optical sectioning and when combined with tissue clearing techniques, it facilitates imaging of centimeter scale specimens with micrometer resolution. Although LSFM is ubiquitous, it still faces two main challenges that effect image quality especially when imaging large volumes with high-resolution.

Illumination angle correction during image acquisition in light-sheet fluorescence microscopy using deep learning.

Light-sheet fluorescence microscopy (LSFM) is a high-speed imaging technique that provides optical sectioning with reduced photodamage. LSFM is routinely used in life sciences for live cell imaging and for capturing large volumes of cleared tissues. LSFM has a unique configuration, in which the illumination and detection paths are separated and perpendicular to each other.

Recent progress in digital holography with dynamic diffractive phase apertures [Invited].

Digital holography with diffractive phase apertures is a hologram recording technique in which at least one of the interfering waves is modulated by a phase mask. In this review, we survey several main milestones on digital holography with dynamic diffractive phase apertures. We begin with Fresnel incoherent correlation holography (FINCH), a hologram recorder with an aperture of a diffractive lens.

Optical incoherent synthetic aperture imaging by superposition of phase-shifted optical transfer functions.

The concept of an optical incoherent synthetic aperture is widely used in astronomical interferometric telescopes. In this Letter, we propose a new, to the best of our knowledge, method to realize optical incoherent synthetic aperture imaging. The method is based on a superposition of optical transfer functions of incoherent imaging systems.

Depth-of-field engineering in coded aperture imaging.

Extending the depth-of-field (DOF) of an optical imaging system without effecting the other imaging properties has been an important topic of research for a long time. In this work, we propose a new general technique of engineering the DOF of an imaging system beyond just a simple extension of the DOF. Engineering the DOF means in this study that the inherent DOF can be extended to one, or to several, separated different intervals of DOF, with controlled start and end points.

Resolution-enhanced imaging using interferenceless coded aperture correlation holography with sparse point response.

Interferenceless coded aperture correlation holography (I-COACH) is a non-scanning, motionless, incoherent digital holography technique. In this study we use a special type of I-COACH in which its point spread hologram (PSH) is ensemble of sparse dots. With this PSH an imaging resolution beyond the classic diffraction limit is demonstrated.

Noise suppression by controlling the sparsity of the point spread function in interferenceless coded aperture correlation holography (I-COACH).

Interferenceless coded aperture correlation holography (I-COACH) is an incoherent opto-digital technique for imaging 3D objects. In I-COACH, the light scattered from an object is modulated by a coded phase mask (CPM) and then recorded by a digital camera as an object digital hologram. To reconstruct the image, the object hologram is cross-correlated with the point spread function (PSF)-the intensity response to a point at the same object's axial location recorded with the same CPM.

Superresolution beyond the diffraction limit using phase spatial light modulator between incoherently illuminated objects and the entrance of an imaging system.

We present a superresolution technique for imaging objects beyond the diffraction limit imposed by the limited numerical aperture (NA) of a general optical system. A coded phase mask (CPM) displayed on a spatial light modulator is introduced between the object and the input aperture of an ordinary lens-based imaging system. Consequently, the effective NA is increased beyond the inherent NA of the optical imaging system.

Resolution enhancement in nonlinear interferenceless COACH with point response of subdiffraction limit patterns.

Interferenceless coded aperture correlation holography (I-COACH) is a non-scanning, motionless, incoherent digital holography technique for 3D imaging. The lateral and axial resolutions of I-COACH are equivalent to those of conventional direct imaging with the same numerical aperture. The main component of I-COACH is a coded phase mask (CPM) used as the system aperture.

Extending the field of view by a scattering window in an I-COACH system.

Interferenceless coded aperture correlation holography (I-COACH) is an incoherent digital holography technique developed to record and reconstruct 3D images of objects without two-wave interference. Herein, we introduce a novel technique to extend the field of view (FOV) of I-COACH beyond the limit imposed by the ratio between the finite area of the image sensor and the magnification of the optical system. Light diffracted from a point object located on the optical axis is modulated by a pseudorandom coded phase mask, and the central part of the point spread hologram (PSH) on the image sensor is recorded.

Single camera shot interferenceless coded aperture correlation holography.

We propose a new scheme for recording an incoherent digital hologram by a single camera shot. The method is based on a motionless, interferenceless, coded aperture correlation holography for 3D imaging. Two random-like coded phase masks (CPMs) are synthesized using the Gerchberg-Saxton algorithm with two different initial random phase profiles.