Soft surface-enhanced Raman scattering sensing platform based on an oil-in-water emulsion stabilized by silver nanoparticles.

Pickering emulsions are excellent candidates for developing soft biosensors utilized for detecting native biomolecules such as peptides and proteins through the Surface-Enhanced Raman Spectroscopy (SERS) transduction mechanism. Here, we have developed a SERS sensor based on oil-in-water Pickering emulsions stabilized by Ag nanoparticles (Ag-NPs) with the Raman active molecule (4-Aminothiphenol, 4ATP) adsorbed to their surface. The structural properties and composition of the Pickering emulsion were tuned to meet the demands of the maximal optical response.

Synthesis, crystal structure and Hirshfeld surface analysis of 4-{(1)-1-[(car-bamo-thioyl-amino)-imino]-eth-yl}phenyl propano-ate.

The title compound, CHNOS, adopts an configuration with respect to the C=N bond. The propionate group adopts an anti-periplanar () conformation. There are short intra-molecular N-H⋯N and C-H⋯O contacts, forming (5) and (6) ring motifs, respectively.

Increasing the survival and efficacy of entomopathogenic nematodes on exposed surfaces by Pickering emulsion formulations offers new venue for foliar pest management.

Formulation technology has been the primordial focus to improve the low viability and erratic infectivity of entomopathogenic nematodes (EPNs) for foliar application. Adaptability to the fluctuating environment is a key trait in ensuring the survival and efficacy of EPNs. Hence, tailoring formulations towards EPNs foliar applications would effectively deliver consistent and reliable results for above-ground applications.

A green formulation for superhydrophobic coatings based on Pickering emulsion templating for anti-biofilm applications.

This study reports significant steps toward developing anti-biofilm surfaces based on superhydrophobic properties that meet the complex demands of today's food and medical regulations. It presents inverse Pickering emulsions of water in dimethyl carbonate (DMC) stabilized by hydrophobic silica (R202) as a possible food-grade coating formulation and describes its significant passive anti-biofilm properties. The final coatings are formed by applying the emulsions on the target surface, followed by evaporation to form a rough layer.

A Novel Platform for Root Protection Applies New Root-Coating Technologies to Mitigate Soil-Borne Tomato Brown Rugose Fruit Virus Disease.

Tomato brown rugose fruit virus (ToBRFV) is a soil-borne virus showing a low percentage of ca. 3% soil-mediated infection when the soil contains root debris from a previous 30-50 day growth cycle of ToBRFV-infected tomato plants. We designed stringent conditions of soil-mediated ToBRFV infection by increasing the length of the pre-growth cycle to 90-120 days, adding a ToBRFV inoculum as well as truncating seedling roots, which increased seedling susceptibility to ToBRFV infection.

Phytocannabinoids Act Synergistically with Non-Steroidal Anti-Inflammatory Drugs Reducing Inflammation in 2D and 3D In Vitro Models.

Lung inflammation is associated with elevated pro-inflammatory cytokines and chemokines. Treatment with FCBD:std (standard mix of cannabidiol [CBD], cannabigerol [CBG] and tetrahydrocannabivarin [THCV]) leads to a marked reduction in the inflammation of alveolar epithelial cells, but not in macrophages. In the present study, the combined anti-inflammatory effect of FCBD:std with two corticosteroids (dexamethasone and budesonide) and two non-steroidal anti-inflammatory drugs (NSAID; ibuprofen and diclofenac), was examined.

Individual Coating of Entomopathogenic Nematodes with Titania (TiO) Nanoparticles Based on Oil-in-Water Pickering Emulsion: A New Formulation for Biopesticides.

This study presents a new eco-friendly formulation of entomopathogenic nematodes (EPNs) based on individual coating of EPNs with titanium dioxide (TiO) nanoparticles (NPs) and mineral oil via oil-in-water Pickering emulsions. Mineral oil-in-water emulsions stabilized by amine-functionalized titanium dioxide (TiO-NH) particles were prepared. 40:60 and 50:50 oil-water volume ratios using 2 wt % TiO-NH particles were found to be the most stable emulsions with a droplet size suitable for the formulation and were further studied for their toxicity against the incorporated EPNs.

Platform for Active Vaccine Formulation Using a Two-Mode Enhancement Mechanism of Epitope Presentation by Pickering Emulsion.

The efficiency of epitope-based vaccination (subunit vaccines) is tightly correlated with heterogeneity and the high density of epitope presentation, which maximizes the potential antigenic determinants. Here, we developed a two-mode platform for intensifying the epitope presentation of subunit vaccines. The two-mode epitope presentation enhancement includes a covalent attachment of high concentrations of SARS-CoV-2-S1 peptide epitope to the surface of virus-like-particles (VLPs) and the subsequent assembly of VLP/epitope conjugates on the oil droplet surface at an oil/water interface of an emulsion as Pickering stabilizers.

Encapsulation of Bacillus thuringiensis in an inverse Pickering emulsion for pest control applications.

Here, we present an inverse Pickering emulsion-based formulation for Bacillus thuringiensis serovar aizawai (BtA) encapsulations utilized towards pest control applications. The emulsification was carried out by high shear homogenization process via ULTRA-TURRAX®. The water-in-mineral oil emulsions were stabilized by commercial hydrophobic silica.

Single-Conidium Encapsulation in Oil-in-Water Pickering Emulsions at High Encapsulation Yield.

This study presents an individual encapsulation of fungal conidia in an oil-in-water Pickering emulsion at a single-conidium encapsulation yield of 44%. The single-conidium encapsulation yield was characterized by analysis of confocal microscopy micrographs. Mineral oil-in-water emulsions stabilized by amine-functionalized titania dioxide (TiO-NH or titania-NH) particles were prepared.

Covalent Immobilization of Polyaniline Doped with Ag or Cu on Carbon Nanotubes for Ethylene Chemical Sensing.

Multi-walled carbon nanotubes (MWCNTs) are promising materials for chemical gas sensing because of their high electrical and mechanical properties and significant sensitivity to changes in the local environment. However, high-content MWCNT films suffer from the low tunability of the electrical resistance, which is crucial for high chemoresistive sensing performance. This study reports the conjugation of MWCNTs and oligomers of polyaniline (PANI) doped with Ag or Cu incorporated into a PVC/polyacrylate.

Fluorine-Free Superhydrophobic Coating with Antibiofilm Properties Based on Pickering Emulsion Templating.

This study presents antibiofilm coating formulations based on Pickering emulsion templating. The coating contains no bioactive material because its antibiofilm properties stem from passive mechanisms that derive solely from the superhydrophobic nature of the coating. Moreover, unlike most of the superhydrophobic formulations, our system is fluorine-free, thus making the method eminently suitable for food and medical applications.

Not Only a Formulation: The Effects of Pickering Emulsion on the Entomopathogenic Action of .

Growing global population and environmental concerns necessitate the transition from chemical to eco-friendly pest management. Entomopathogenic fungi (EPF) are rising candidates for this task due to their ease of growing, broad host range and unique disease process, allowing EPF to infect hosts directly through its cuticle. However, EPF's requirement for high humidity negates their integration into conventional agriculture.

Cannabis compounds exhibit anti-inflammatory activity in vitro in COVID-19-related inflammation in lung epithelial cells and pro-inflammatory activity in macrophages.

Cannabis sativa is widely used for medical purposes and has anti-inflammatory activity. This study intended to examine the anti-inflammatory activity of cannabis on immune response markers associated with coronavirus disease 2019 (COVID-19) inflammation. An extract fraction from C.

A Robust Fabrication Method for Amphiphilic Janus Particles via Immobilization on Polycarbonate Microspheres.

Immobilizing particles on beads, fibers, or filaments, when only one side is exposed to the reaction medium and therefore can be selectively functionalized, is a scalable and easy to control strategy for the fabrication of amphiphilic Janus particles. Here we describe a new, robust method for the fabrication of amphiphilic Janus particles based on immobilization of polymethylsilsesquioxane (PMSQ) particles on polycarbonate (PC), a high impact-resistance polymer with superior mechanical properties. The immobilization of the particles on the PC microspores is performed via inverse solvent displacement method.

Crystal structures of ()-4-[1-(2-carbamo-thio-yl-hydrazinyl-idene)eth-yl]phenyl acetate and ()-4-[1-(2-carbamo-thio-ylhydrazinyl-idene)eth-yl]phenyl benzoate.

In the title compounds, CHNOS, (I), and CHNOS, (II), the thio-semicarbazone group adopts an extended conformation. The acetate ester (I) crystallizes with two independent mol-ecules in the asymmetric unit. In the benzoate ester (II), the planes of the two aryl rings are inclined to one another by 46.

Determination on the binding of thiadiazole derivative to human serum albumin: a spectroscopy and computational approach.

4-[3-acetyl-5-(acetylamino)-2,3-dihydro-1,3,4-thiadiazole-2-yl]phenyl benzoate from the family of thiadiazole derivative has been newly synthesized. It has good anticancer activity as well as antibacterial and less toxic in nature, its binding characteristics are therefore of huge interest for understanding pharmacokinetic mechanism of the drug. The binding of thiadiazole derivative to human serum albumin (HSA) has been investigated by studying its quenching mechanism, binding kinetics and the molecular distance, r between the donor (HSA) and acceptor (thiadiazole derivative) was estimated according to Forster's theory of non-radiative energy transfer.

Insights into the binding of thiosemicarbazone derivatives with human serum albumin: spectroscopy and molecular modelling studies.

4-[(1Z)-1-(2-carbamothioylhydrazinylidene)ethyl]phenyl acetate [Ace semi],4-[(1Z)-1-(2-carbamothioylhydrazinylidene)ethyl]phenyl propanoate [Pro semi] from the family of thiosemicarbazones derivative has been newly synthesized. It has good anticancer activity as well as antibacterial and it is also less toxic in nature, its binding characteristics are therefore of huge interest for understanding pharmacokinetic mechanism of the drug. The binding of thiosemicarbazone derivative to human serum albumin (HSA) has been investigated by studying its quenching mechanism, binding kinetics and the molecular distance (r) between donor (HSA) and acceptor (thiosemicarbazone derivative) was estimated according to Forster's theory of non-radiative energy transfer using fluorescence spectroscopy.

Crystal structure of (E)-4-{1-[2-(car-bamo-thio-yl)hydrazin-1-yl-idene]ethyl}phenyl 4-methyl-benzoate.

The asymmetric unit of the title compound, C17H17N3O2S, consists of two independent mol-ecules, A and B, with different conformations: in mol-ecule A, the dihedral angles between the central benzene ring and the pendant tolyl and carbamo-thio-ylhydrazono groups are 71.12 (9) and 5.95 (8)°, respectively.

Crystal structure of 4-acetyl-phenyl 3-methyl-benzoate.

The planes of the aromatic rings of the title compound, C16H14O3, make a dihedral angle of 82.52 (8)°. The acetyl group and the phenyl ring make a dihedral angle of 1.