Gene Knockout Reduces the Metabolism and Efficacy of Venlafaxine in Rats.

Rat has been considered as an ortholog of human To assess the role of CYP2D1 in physiologic processes and drug metabolism, a -null rat model was generated with a CRISPR/Cas9 method. Seven base pairs were deleted from exon 4 of of Sprague-Dawley wild-type (WT) rats. The -null rats were viable and showed no abnormalities in general appearance and behavior.

Generation and Characterization of a CYP2C11-Null Rat Model by Using the CRISPR/Cas9 Method.

CYP2C11 is involved in the metabolism of many drugs in rats. To assess the roles of CYP2C11 in physiology and drug metabolism, a -null rat model was generated using the clustered

A Novel LC-MS-MS Method With an Effective Antioxidant for the Determination of Edaravone, a Free-Radical Scavenger in Dog Plasma and its Application to a Pharmacokinetic Study.

The objective of this study was to investigate the stability of edaravone in dog plasma by using an added antioxidant stabilizer, with an ultimate goal of developing and validating a sensitive, reliable and robust LC-MS-MS method for determination of edaravone in plasma samples. Edaravone was unstable in plasma, but it presented a good stability performance in the plasma with sodium metabisulfite (SMB), an effective antioxidant. The blood sample was collected in the heparinized eppendorf tube containing SMB and plasma sample was deproteinized using acetonitrile containing 20 ng/mL of phenacetin (Internal standard).

Methylmercury inhibits dopaminergic function in rat pup synaptosomes in an age-dependent manner.

Methylmercury (MeHg) is an environmental neurotoxicant that is especially harmful during brain development. Previously, we found greater sensitivity to MeHg-induced oxidative stress and greater loss of mitochondrial membrane potential in synaptosomes from early postnatal rats than in synaptosomes from older rat pups and adults. Here, we determine whether MeHg exposure also leads to greater changes in dopamine (DA) levels and dopamine transporter (DAT) function in synaptosomes from early postnatal rats.