Fourier transform infrared spectroscopy evaluation of low density lipoprotein oxidation in the presence of quercetin, catechin, and alpha-tocopherol.

We investigated the changes in human LDL primary and secondary lipid oxidation products and modification of the apolipoprotein B-100 (apoB-100) secondary structures during Cu2+-mediated oxidation by FTIR spectroscopy in the presence of catechin, quercetin, and alpha-tocopherol at physiological concentrations. Catechin- and quercetin-containing samples had slower rates and longer lag phases for conjugated diene hydroperoxide (CD) formation than alpha-tocopherol-containing samples; however, all antioxidant-treated LDL samples generated similar CD levels (P< 0.05).

Quantitative determination of low density lipoprotein oxidation by FTIR and chemometric analysis.

This study was conducted to develop a quantitative FTIR spectroscopy method to measure LDL lipid oxidation products and determine the effect of oxidation on LDL lipid and protein. In vitro LDL oxidation at 37 degrees C for 1 h produced a range of conjugated diene (CD) (0.14-0.