Publications by authors named "Manem Krishnareddy"
Article Synopsis
- A cellobiose phosphorylase (CBP) from Clostridium thermocellum was purified and studied for its reaction mechanisms, showing an ordered bi bi mechanism where P(i) binds first.
- The enzyme demonstrated a preference for beta-D-glucose over alpha-D-glucose, highlighting the importance of the beta-anomeric configuration for its activity, although some activity remains without it.
- Substrate specificity tests showed that the configurations of the C3 and C4 hydroxyl groups are essential for enzyme function, while variations in C2 and C6 positions are permissible; D-glucose was found to inhibit the enzyme by competing with another substrate, G 1-P.
