Multifaceted computational profiling of thymol and geraniol against the human proteome for bio-repellent alternatives: Toxicity predictions, degradation analysis, and quantum mechanical approaches.

With growing interest in natural compounds as alternative mosquito repellents, assessing the toxicity and structure of potential repellent naturals like thymol (monoterpene phenol) and geraniol (monoterpene alcohol) is vital for understanding their stability and human impact. This study aimed to determine the structural, toxicity, and binding profiles of thymol and geraniol using computational predictions, xTB metadynamics, quantum mechanics, and principal component analysis. Toxicity studies using Protox-II, T.

Comprehensive Molecular Interaction Studies to Construe the Repellent/Kill Activity of Geraniol During Binding Event Against Aedes aegypti Proteins.

Aedes aegypti is an etiological agent for dengue, chikungunya, zika, and yellow fever viruses. With the advent of the use of natural alternatives as repellents, their precise mode of action during the event of binding is still unclear. Geraniol is one such bioactive natural that has been previously shown to have some insecticide properties.

High concentrations of membrane-fed ivermectin are required for substantial lethal and sublethal impacts on Aedes aegypti.

Background: With widespread insecticide resistance in mosquito vectors, there is a pressing need to evaluate alternatives with different modes of action. Blood containing the antihelminthic drug ivermectin has been shown to have lethal and sub-lethal effects on mosquitoes. Almost all work to date has been on Anopheles spp.

ZnO-SiO nanohybrid decorated sustainable geopolymer retaining anti-biodeterioration activity with improved durability.

Geopolymer, consists of industrial by-product fly ash, and alkaline activator, possesses similar strength along with durability like conventional cement composite, is an alternative construction substantial of Portland cement in current scenario. Corrosion of the concrete materials resulted mainly from the chemical degradation. Besides chemical degradation, biogenic-deterioration is also another alarming issue especially in the sewer systems, bridge piers, several pipelines and offshore platforms and the need to act on it is long-standing.

Comparative Bio-Efficacy and Synergism of New Generation Polyfluorobenzyl and Conventional Pyrethroids Against Culex quinquefasciatus (Diptera: Culicidae).

Intensive exposure to insecticides has resulted in the evolution of insecticide resistance in the mosquitoes. We tested the bio-efficacy of two Culex quinquefasciatus Say (Diptera: Culicidae) laboratory strains differentially bio-responsive to pyrethroids to understand the comparative efficacy of different polyfluorobenzyle and conventional pyrethroid molecules and the role of piperonyl butoxide (PBO) in synergizing these molecules in increased tolerance of mosquitoes to these molecules. We have taken deltamethrin (α-cyano pyrethroid with phenoxybenzyl moiety); permethrin (phenoxybenzyl pyrethroid without an α-cyano group); transfluthrin, dimefluthrin, metofluthrin, and meperfluthrin (polyfluorinated benzyl compounds); and prallethrin (modified cyclopentadienone compound) for this study.

Iron Oxide NPs Facilitated a Smart Building Composite for Heavy-Metal Removal and Dye Degradation.

Due to the growing population, drought, and the contamination of conventional water sources, the need for clean water is rising worldwide with high demand. The application of nanomaterials for water purification can provide a better water quality, by eliminating toxic metals and also decomposing organic contaminants. Exploitation of industrial coal-burned byproduct, fly ash, through nanomodification has been developed in this exertion for the treatment of wastewater along with heavy-metal remediation and dye degradation.

Participatory role of zinc in structural and functional characterization of bioremediase: a unique thermostable microbial silica leaching protein.

A unique protein, bioremediase (UniProt Knowledgebase Accession No.: P86277), isolated from a hot spring bacterium BKH1 (GenBank Accession No.: FJ177512), has shown to exhibit silica leaching activity when incorporated to prepare bio-concrete material.

Fatty acid profiling and molecular characterization of some freshwater microalgae from India with potential for biodiesel production.

We determined the fatty acid compositions of six species of freshwater microalgae belonging to the Chlorophyta, which were isolated from freshwater bodies in Assam, India. All six microalgae -Desmodesmus sp. DRLMA7, Desmodesmus elegans DRLMA13, Scenedesmus sp.

Multiple insecticide resistance/susceptibility status of Culex quinquefasciatus, principal vector of bancroftian filariasis from filaria endemic areas of northern India.

Objective: To understand the insecticide resistance status of Culex quinquefasciatus Say (Diptera: Culicidae) (Cx. Quinquefasciatus) to deltamethrin, cyfluthrin, permethrin, lambdacyhalothrin, DDT and malathion in filarial endemic areas of Uttar Pradesh, India.

Methods: Insecticide susceptibility assays were performed on wild-caught adult female Cx.

High-throughput approach to detection of knockdown resistance (kdr) mutation in mosquitoes, Culex quinquefasciatus, based on real-time PCR using single-labelled hybridisation probe/melting curve analysis.

Background: Knockdown resistance (kdr) mutation (L1014F) is a well-defined mechanism of resistance to pyrethroids and DDT in many insect species. Sensitive detection of the mutations associated with resistance is a prerequisite for resistance management strategies. The authors have developed a new real-time molecular diagnostic assay based on SimpleProbe(®)/melting curve analysis for large-scale kdr genotyping in the wild population of Culex quinquefasciatus Say, the principal vector of bancroftian filariasis.

Development of physiological resistance and its stage specificity in Culex quinquefasciatus after selection with deltamethrin in Assam, India.

The study investigated the development and stage specificity of physiological resistance to insecticides in a colony of Culex quinquefasciatus Say (Diptera: Culicidae) mosquitoes, which are vectors of bancroftian filariasis in India, after selection with deltamethrin. Resistance was selected by exposing the larvae to the concentration of deltamethrin that caused 50% mortality in the tested population (i.e.

Molecular analysis of knock down resistance (kdr) mutation and distribution of kdr genotypes in a wild population of Culex quinquefasciatus from India.

Objective: To investigate the presence of knock down resistance (kdr) mutation, its frequency distribution in the principal vector of bancroftian filariasis, Culex quinquefasciatus from northeastern India, and to relate kdr genotypes with susceptibility and/or resistance to DDT and deltamethrin in this vectors.

Methods: Adult female mosquitoes were collected by aspiration from human dwellings in two villages, Benganajuli and Rikamari, and two military establishments, Field Units I and II. Insecticide susceptibility tests were performed following WHO methods with 4% DDT and 0.

Parasitosis: a study among Nepali children in the district of Darjeeling.

A cross-sectional study carried out among 370 school-going children of the Nepali community, aged 5-10 years, in the Siliguri subdivision of Darjeeling district revealed the prevalence of parasitic infestation to be 51.4 per cent of which 28.2% and 23.