Publications by authors named "Manar Magdy Mahmoud Mohamed"
Article Synopsis
- * Researchers fermented IMET 43975 to isolate five known and four new ansamycin analogues, including new compounds called hygrocins.
- * The structural analysis of these ansamycins was conducted using advanced techniques, and a specific enzyme involved in their formation was confirmed through CRISPR base editing, enhancing our understanding of their diversity.
Cinnamoyl moiety containing nonribosomal peptides represented by pepticinnamin E are a growing family of natural products isolated from different species and possess diverse bioactivities. The soil bacterium P8-A2 harbors a cryptic pepticinnamin biosynthetic gene cluster, producing azodyrecins as major products. Inactivation of the azodyrecin biosynthetic gene cluster by CRISPR-BEST base editing led to the activation and production of pepticinnamin E () and its analogues, pepticinnamins N, O, and P (-), the structures of which were determined by detailed NMR spectroscopy, HRMS data, and Marfey's reactions.
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