Preparing polycaprolactone scaffolds using electrospinning technique for construction of artificial periodontal ligament tissue.

Objectives: The strategies of tissue-engineering led to the development of living cell-based therapies to repair lost or damaged tissues, including periodontal ligament and to construct biohybrid implant. This work aimed to isolate human periodontal ligament stem cells (hPDLSCs) and implant them on fabricated polycaprolactone (PCL) for the regeneration of natural periodontal ligament (PDL) tissues.

Methods: hPDLSCs were harvested from extracted human premolars, cultured, and expanded to obtain PDL cells.