Publications by authors named "Manami Tabira"
J Biosci Bioeng
January 2024
Article Synopsis
- The study addresses the challenge of mutating both allelic genes in industrial shochu yeast, a diploid strain, using a novel genetic disruption method.
- Researchers created a disruption cassette featuring an intact gene (LYS5) alongside nonfunctional gene fragments (ura3) that facilitated gene removal and regeneration through a looping mechanism.
- The approach successfully resulted in double-disrupted strains of shochu yeast, showcasing the effectiveness and repeatability of this method for genetic manipulation in Saccharomyces cerevisiae.
