Binding affinities of paclitaxel and docetaxel for generic and nanoparticle albumin-bound paclitaxel-derived albumin from human serum.

Nanoparticle albumin-bound (nab)-paclitaxel is a 130-nm formulation containing human serum albumin (HSA). The clinical efficacy of this formulation is considered to depend on its affinity for HSA. The high pressure employed during the manufacture of nab-paclitaxel HSA (nab HSA) may influence its conformation and/or oligomerization, and ultimately its affinity for HSA.

Robust Nanoparticle Morphology and Size Analysis by Atomic Force Microscopy for Standardization.

Because of the complexity of nanomedicines, analysis of their morphology and size has attracted considerable attention both from researchers and regulatory agencies. The atomic force microscope (AFM) has emerged as a powerful tool because it can provide detailed morphological characteristics of nanoparticles both in the air and in aqueous medium. However, to our knowledge, AFM methods for nanomedicines have yet to be standardized or be listed in any pharmacopeias.

Effects of Contact Angle and Flocculation of Particles of Oligomer of Tetrafluoroethylene on Oil Foaming.

Oil foams have been stabilized by using particles of oligomer of tetrafluoroethylene (OTFE). OTFE particles were dispersed in oil mixtures prior to aeration, to exclude the oil-repellency nature of the particles due to the formation of the metastable Cassie-Baxter state and properly evaluate the effects of contact angle on the foaming behavior. The particle contact angle (θ) against air/oil surfaces were controlled by changing a composition of two oils with different surface tension (-heptane and methyl salicylate).

Water-proton relaxivities of DNA oligomers carrying TEMPO radicals.

5-Uridine derivative carrying a TEMPO radical (UST) was prepared and its single strand (ssUST) and a double strand (dsUST) with its complementary strand were obtained. Similarly, single strands carrying two and five radicals (ssUST2 and ssUST5, respectively) and the corresponding double strands (dsUST2 and dsUST5) were prepared. Their electron paramagnetic resonance (EPR) spectra showed typical anisotropic broadening in the high field line.

Synthesis and relaxivities of oligonucleotides with TEMPO-labeled sugar moiety.

5-Uridin derivative, UUT, having TEMPO radical at the 2'-position of sugar moiety, was prepared and characterized by X-ray crystallography. The single strands, ssUUT, incorporating UUT and the double strands, dsUUT, with complimentary strand were also obtained. Their EPR spectra and relaxivities (25 MHz and 0.