[Application of monoclonal antibody immobilized polyurethane film for site-specific gene therapy].

Objective: To study the feasibility of delivering viral gene vector from a collagen-coated polyurethane (PU) film through a mechanism involving monoclonal antiviral antibody tethering.

Methods: Anti-adenoviral monoclonal antibodies were covalently bound to the collagen-coated PU surface. These antibodies enabled tethering of replication defective adenoviruses through highly specific antigen-antibody affinity.

New method for preparing more stable microcapsules for the entrapment of genetically engineered cells.

In this paper, we studied a new preparation method of microcapsules for entrapment of genetically engineered cells. Polyvinyl alcohol microcapsules having well defined shape, high mechanical strength, good biochemical and permeability properties were prepared by using low temperature physical cross-linking method. Comparing with currently used alginate-polylysine-alginate microcapsules, polyvinyl alcohol microcapsules have much higher mechanical strength.