Molecular characterization and hydrogen production of a new species of anaerobe.

For the fermentative hydrogen production process with carbohydrates, isolation and identification of hydrogen-producing bacteria (HPB) with high yield and high evolution rate are very important. Improved Hungater rolling tube technique and plate method of culture bottle (PMCB) were used to enumerate and isolate the HPB. The HPB-Li and Ren (HPB-LR) medium was designed to inoculate and isolate HPB under temperature of 37 degrees C and pH of 4.