Bioassay-guided isolation and in Silico characterization of cytotoxic compounds from Hemimycale sp. Sponge targeting A549 lung cancer cells.

Bioassay-guided fractionation approach led to identification of two novel compounds; (4-(hydroxymethyl)-3-methoxy-1H-pyrazol (1) and mycalene (2), alongside with four known metabolites; octadecane (3), hexatriacontane (4), 1-heneicosanol (5) and heptatriacontanoic acid (6) from the Red Sea marine sponge Hemimycale sp. The ethyl acetate fraction showed a noticeable cytotoxic activity against the lung cancer cell line (A549) with IC value of 75.54 µg/ mL.

New sesquiterpenes from the soft coral Litophyton arboreum.

Two new sesquiterpenes; 8α,11-dihydroxy-β-cyperon (2), and 5-epi-7α-hydroxy-( +)-oplopanone (3), were isolated from the soft coral Litophyton arboreum, together with nine known ones, including five sesquiterpenes; 11-hydroxy-8-oxo-β-cyperon (1), alismoxide (4), 5β,8β-epidioxy-11-hydroxy-6-eudesmene (5), chabrolidione B (6), 7-oxo-tri-nor-eudesm-5-en-4β-ol (7), two sterols; 7β-acetoxy-24-methyl-cholesta-5,24(28)-diene-3β,19-diol (8), nebrosteroid M (9), and two glycerol derivatives; chimyl alcohol (10) and batyl alcohol (11). The structures of the isolated compounds were characterized using spectroscopic techniques, predominately HR-ESI-MS, 1D, 2D-NMR, and ECD analyses. Compounds 1-11 were evaluated for their cytotoxic activity against three human cancer-cell lines (A549, MCF-7 and HepG2), and anti-leishmanial potential against the causal parasite, Leishmania major.

Isolation and chemical characterization of secondary metabolites from and their antimicrobial and cytotoxic activities.

Phytochemical study of whole plant led to isolation and structural characterisation of six known compounds including lupeol (), lupenone (), -sitosterol 3---glucopyranoside (), dunalianoside D (), 3,5-di--caffeoylquinic acid (), and tachioside (). Compounds (, and ) were isolated for the first time. The structures of the isolated compounds were elucidated using different spectroscopic analyses such as H,C, and DEPT NMR analyses, besides HR-ESI-MS analysis.

Cytotoxic metabolites from Sinularia levi supported by network pharmacology.

The in-vitro anti-proliferative evaluation of Sinularia levi total extract against three cell lines revealed its potent effect against Caco-2 cell line with IC50 3.3 μg/mL, followed by MCF-7 and HepG-2 with IC50 6.4 μg/mL and 8.

Niosomes as promising approach for enhancing the cytotoxicity of Hemimycale sp. total crude extract supported with in-silico studies.

The crude extract of Hemimycale sp. marine sponge was evaluated as a cytotoxic drug against different cell lines; whereas it exhibited promising selective activity toward the breast cancer cell line only with IC value 199.6 ± 0.

Anti-leishmanial and cytotoxic compounds isolated from marine sponge .

The methanolic extract of the marine sponge . yielded two new compounds; 1-(2'-methyl heptadecyl) phenol () and a new pyrazole derivative; 4-(hydroxymethyl)-1-pyrazol-3-ol (), together with previously isolated (2')-2'-hydroxy-N-((2,3,4)-1,3,4-trihydroxy-16-methylpentadecan-2-yl)docosanamide (), cholesterol (), 5, 8-dioxycholest-6-en-3-ol () and 3-acetylsesterstatin 3 (), which were firstly reported from family Hymedesmiidae. Their structure elucidation was based on extensive nuclear magnetic resonance spectroscopy and high resolution-electrospray ionization-mass spectrometry.

Ultrastructural, Energy-Dispersive X-ray Spectroscopy, Chemical Study and LC-DAD-QToF Chemical Characterization of (L.) Ach.

The lichen (L.) Ach. has been used in traditional and modern medicines for its many biological properties such as immunological, immunomodulating, antioxidant, antimicrobial, and anti-inflammatory activities.

Metabolomic profiling and biological evaluations of Spongia irregularis-associated actinomycetes supported by multivariate statistical analysis.

Aim: Metabolomic analysis using LC-HRESIMS of 12 extracts of Spongia irregularis-associated actinomycetes for dereplication purposes in addition to evaluation of cytotoxic and antiviral activities of the extracts.

Methods And Results: In this study, three actinomycetes belonging to the genera Micromonospora, Streptomyces, and Rhodococcus were recovered from the marine sponge Spongia irregularis. Applying the OSMAC approach, each strain was fermented on four different media, resulting in 12 extracts.

Isolation and LC-QToF Characterization of Secondary Metabolites from an Endemic Plant Poljak.

Phytochemical investigation of the aerial parts of Poljak led to the isolation of ten known compounds, including four alkyl -coumarates: octadecyl --coumarate (), icosy --coumarate (), docosyl --coumarate (), and tetracosyl --coumarate (), one sesquiterpene lactone: santonin (), four flavonoids; axillarin (), quercetin 3--methyl ether (), luteolin (), and quercetin (), and one phenolic acid derivative: -coumaric acid (). The structures of the isolated compounds were identified by various spectroscopic analyses. Additionally, the antimicrobial activity of the total extract and different fractions was screened, and they exhibited no inhibition of the growth of , , , methicillin-resistant (MRS), , , , and Vancomycin-resistant (VRE) at the tested concentrations ranging from 8 to 200 μg/mL.

Bioassay-Guided Fractionation with Antimalarial and Antimicrobial Activities of .

Bioassay-guided fractionation technique of roots of led to isolation and structure elucidation of seven known compounds, including four monoterpene glycosides: lactiflorin (), paeoniflorin (), galloyl paeoniflorin (), and ()-(1,5)--pinen-10-yl -vicianoside (); two phenolics: benzoic acid () and methyl gallate (); and one sterol glycoside: -sitosterol 3---D-glucopyranoside (). The different fractions and the isolated compounds were evaluated for their antimicrobial and antimalarial activities. Fraction II and III showed antifungal activity against with IC values of 28.

Aureanin: a new iridoid from the leaves of (Silva Manso) Benth. & Hook.f. ex S.Moore.

One new iridoid named aureanin () was isolated from the leaves of (Silva Manso) Benth. & Hook.f.

Isolation and structural characterization of phytoconstituents from leaves of .

Phytochemical investigation of leaves led to the isolation of three new compounds: including a glycoside of simple alcohol, namely binatoside (), 3,4-dihydroxy--methyl piperidin-2-one (), and a phenyl ethanoid glycoside, namely bignanoside C (), alongside with five known compounds; including a glycoside of simple alcohol; (2) propane-1,2-diol 1--(6--caffeoy1)--D-glucopyranoside (), phenyl ethanoids; leucosceptoside A () and plantainoside C (), and iridoids; ipolamiide () and strictoloside (). The structure of the isolated compounds was elucidated by various spectroscopic methods, including 1 D and 2 D NMR experiments, HR-ESI-MS as well as by comparison with the literature.

NS3/4A helicase inhibitory alkaloids from as HCV target.

Chemical investigation of roots extract, using chromatographic and spectroscopic techniques, resulted in isolation and identification of eight known compounds. The basic ethyl acetate fraction (alkaloidal fraction) afforded -methylsceletenone, epinine, 4-methoxy phenethylamine, and -methyl tyramine while, the acidic ethyl acetate fraction (non-alkaloidal fraction) afforded only -coumaroyl tyramine. Moreover, the petroleum ether fraction afforded capric acid, tricosanol, and a mixture of β-sitosterol & stigma sterol.

NS3 helicase inhibitory potential of the marine sponge .

In the current study, an investigation of the activity of the total extract of the marine sponge and its different fractions against the hepatitis C virus (HCV) was pursued. The results revealed that the ethyl acetate fraction exhibited the highest anti-HCV activity, with an IC value of 12.6 ± 0.

Cytotoxic and antileishmanial triterpenes of (Silva Manso) leaves.

The bioactivity-guided fractionation of the total ethanolic extract of the leaves of revealed the cytotoxic and antileishmanial potency of the ethyl acetate fraction, in which its phytochemical investigation resulted in the isolation of five triterpenes; identified as oleanolic acid (), ursolic acid (), pomolic acid (), tormentic acid (), 3,6,19-trihydroxy-urs-12-en-28-oic acid () in addition to one triterpenoid glucoside, spathodic acid 28---D-glucopyranoside (). Whereas compound showed cytotoxic activity against three different cell lines; A549, MCF-7 and HepG2 with IC values of 31.7 ± 1.

The Red Sea marine sponge : metabolomic profiling and cytotoxic potential supported by studies.

The current study discusses the chemical composition of the marine sponge using LC-HRESIMS. The metabolomic profiling resulted in the annotation of 17 metabolites of different chemical classes. Additionally, evaluation of the cytotoxic activities of the total extract and different fractions were carried out against three different cell lines where the -butanol fraction exhibited the highest cytotoxic effects against HepG-2, MCF-7 and CACO-2 cell lines with IC values of 9.

Cytotoxicity and chemical profiling of the Red Sea soft corals .

The objective of this research was to evaluate the cytotoxic activities of the fractions and isolated compounds of the soft corals against A549, MCF-7 and HepG2 cell lines by MTT assay method, and to chemically investigate the various metabolites of its total extract using LC-HR-ESI-MS metabolomic profiling. The metabolomic profiling revealed the presence of various metabolites, mainly sesquiterpenes and steroids reported for the first time in Additionally, eight compounds (-) have been isolated from the -hexane-chloroform (1:1) fraction that exhibited noticeable activity towards A549, MCF-7 and HepG2 cell lines. The steroids ( and ), and the sesquiterpene () exerted noticeable activity against A549 cell line (IC 28.

Recent Updates on Sinularia Soft Coral.

Marine organisms are recognized as a rich source of bioactive secondary metabolites. The remarkable abundance and diversity of bioactive small molecules isolated from soft corals displayed their essential role in drug discovery for human diseases. Sterols and terpenes, particularly cembranolides, 14-membered cyclic diterpene, demonstrated numerous biological activities, such as antitumor, antimicrobial, antiviral, antidiabetic, anti-osteoporosis and anti-inflammatory.

Gas chromatography-mass spectrometry profiling and analgesic, anti-inflammatory, antipyretic, and antihyperglycemic potentials of .

Objectives: The present study determines the chemical constituents of using gas chromatography-mass spectrometry (GC-MS) and its different activities.

Materials And Methods: Air-dried powdered leaves of were extracted by 95% methanol and fractionated consecutively with petroleum ether, chloroform, and ethyl acetate. The saponifiable matter, EtOAc and aqueous fractions were subjected to GC-MS.

Elastase inhibitory activity of secondary metabolites from the fungus CF-231658.

Three known compounds were isolated from CF-231658; 2,7-dihydroxy naphthalene (), virgaricin B () and virgaricin (). The isolated compounds was obtained from liquid-state and agar-supported fermentation using Amberlite XAD-16 solid-phase extraction during the cultivation step. Their structures were elucidated on the basis of 1D and 2D NMR as well as HRMS spectroscopic analyses.

LC-MS-based identification of bioactive compounds and hepatoprotective and nephroprotective activities of leaves against carbon tetrachloride-induced injury in rats.

The chemical profiling of the main phytoconstituents of total ethanolic extract (TEE) and its different fractions of leaves was dereplicated using liquid chromatography-high resolution-electrospray ionisation-mass spectrometry (LC-HR-ESI-MS), revealed the presence of various classes of secondary metabolites; eight phenylethanoids, two flavonoidal glycosides and two iridoids. Moreover, the hepatoprotective and nephroprotective activities of the TEE and its different fractions were investigated in carbon tetrachloride (CCl)-intoxicated rats and were compared with those of silymarin-treated group, revealing the highest potency of the EtOAc group, followed by the aqueous one in improving the CCl-induced alterations in several biochemical parameters. Besides, EtOAc and aqueous fractions exhibited the most inhibition of CCl-induced inflammatory mediators and improving the changes in the histopathological structures of the liver and kidney.

Marine natural products from sponges (Porifera) of the order Dictyoceratida (2013 to 2019); a promising source for drug discovery.

Marine organisms have been considered an interesting target for the discovery of different classes of secondary natural products with wide-ranging biological activities. Sponges which belong to the order Dictyoceratida are distinctly classified into 5 families: Dysideidae, Irciniidae, Spongiidae, Thorectidae, and Verticilliitidae. In this review, compounds isolated from Dictyoceratida sponges were discussed with their biological potential within the period 2013 to December 2019.