Antimicrobial resistance and molecular epidemiological typing of Neisseria gonorrhoeae isolates from Kyrgyzstan in Central Asia, 2012 and 2017.

Background: Gonorrhoea and antimicrobial resistance (AMR) in Neisseria gonorrhoeae are significant public health concerns globally. Nearly no gonococcal AMR data are available from Central Asia, and no data from Kyrgyzstan has been published. We examined, for the first time, AMR and molecular epidemiology of N.

[The effect of NADP+ on electrophysiological properties of cardiomyocytes of C57BL/6 and mdx mice].

We have studied the influence of NADP+ on routine ECG in 6 months old C57BL/6 and mdx mice. The animals were anestheized by ether before ECG recording. ECG registration was made with the speed of 100 mm per sec.

[Avandia and avandamet in the treatment of patients with diabetes mellitus type 2: effects on secretion of some fat tissue hormones].

Aim: To study effects of avandia and its combination with metformine (avandamet) on secretion of fat tissue hormones.

Material And Methods: The examination protocol for 42 patients with type 2 diabetes mellitus (DM) aged 62.4 +/- 7.

[Polymorphic locus D6S392 near the Mn-dependent superoxide dismutase gene is associated with diabetes mellitus in the Moscow population].

Aim: Distribution of alleles of tetranucleotide microsatellite D6S392 located nearby the gene of Mn-dependent superoxide dismutase (SOD2) was studied in healthy donors (n = 143), patients with insulin-dependent (n = 166) and insulin-independent (n = 101) diabetes mellitus (IDDM and IIDM).

Materials And Methods: Alleles of the polymorphic locus D6S392 were amplified using polymerase chain reaction (PCR) on the basis of genome DNA isolated from the venous blood of the examinees. PCR products were analysed with gel-electrophoresis in polyacrylamide gel.

[Analysis of polymorphism of the D11S2008 locus of the catalase gene in patients with hypertension and ischemic heart disease in non-insulin-dependent diabetes mellitus in the Muscovite population].

The allele and genotype frequency distributions of the D11S2008 tetranucleotide microsatellite linked with the catalase (CAT) gene were compared between patients with insulin-dependent diabetes mellitus (IDDM) with (N = 72) and without (N = 82) coronary heart disease (CHD), and between IDDM patients with normal arterial tension (N = 82) and with arterial hypertension (N = 42). In total, eight alleles were found. The alleles varied in length from 120 to 148 bp and included from 15 to 22 tetranucleotide repeats.

[Polymorphism of the angiotensin I-converting enzyme gene in diabetic retinopathy patients and type 2 diabetes mellitus patients with myocardial infarction].

The insertion/deletion polymorphism (I/D) of the angiotensin I-converting enzyme gene (ACE) was examined in type I diabetes mellitus patients (DM) with (n = 31), or without (n = 33) retinopathy, and in type 2 DM patients with either myocardial infarction (MI; n = 75), or with (n = 37), or without (n = 178) retinopathy. No association between the ACE gene and retinopathy in type 1 and type 2 DM patients was revealed. In the type 2 DM patients with MI, a statistically significant (P < 0.

[Clinical and epidemiological aspects of diabetic retinopathy and its relationship with diabetic nephropathy].

Results of clinical and laboratory examinations of 161 diabetics are presented. The main factors or risk of nonproliferative diabetic retinopathy are the duration and degree of compensation of diabetes mellitus, development and stage of diabetic nephropathy, the latter factor replacing in experiments with simulation of diabetic retinopathy the level of arterial hypertension, and the blood serum content of high-density lipoprotein cholesterol and ratio of total cholesterol to high-density lipoprotein cholesterol. The factors of risk of proliferative diabetic retinopathy are duration and degree of compensation of diabetes mellitus, development and stage of diabetic retinopathy with this latter factor replacing in simulation of diabetic retinopathy the level of arterial hypertension, and the blood serum levels of triglycerides, low-density lipoprotein cholesterol, fibrinogen, soluble fibrin-monomer complexes, as well as fibrinolytic activity.

[Kidney lesions in non-insulin dependent diabetes mellitus].

Because little is known about disordered metabolism of uric acid as a pathogenetic mechanism of renal damage in non-insulin-dependent diabetes mellitus (NIDDM) it was studied in 75 NIDDM and 48 IDDM patients. Clinical examination of the patients included evaluation of purin metabolism according to serum uric acid, diurnal urine excretion, uric acid clearance. Hyperuricemia occurred more frequently in NIDDM.

[Use of laser doppler flowmeter for the early diagnosis of diabetic microangiopathies].

Use of laser doppler flowmeter helped reveal in patients with diabetes mellitus a decrease of volumetric bloodstream in the capillaries of skin in the absence of clinical and biochemical evidence of microcirculatory disorders. Bloodstream parameters were correlated to the findings of objective methods of diagnosis at the stage of manifest clinical signs of vascular involvement of various localizations. Laser doppler flowmeter may be used for preclinical diagnosis of renal and ophthalmic involvement in diabetics with types I and II conditions as a method to monitor the therapy efficacy in patients with apparent clinical signs of microangiopathies.

[Immunologic aspects of renal involvement in patients with type II diabetes mellitus].

Besides the known factors contributing to the pathogenesis of diabetic nephropathy, the role of immune mechanisms in types I and II diabetes is discussed of late; the contribution of autoimmune mechanisms to pathogenesis of noninsulin-dependent diabetes (NIDDM) is virtually unknown. Seventy-six patients with NIDDM and 48 with insulin-dependent condition were examined. Under study were levels of antibodies to FxIA and renal glomerular basal membrane antigens in the blood sera of donors and patients with types I and II diabetes, as well as concentrations, size, and pathogenicity of immune complexes.

[Hyperplastic ovarian processes from the clinico-morphological aspect].

Clinico-morphologic analysis was carried out in 93 patients with hyperplastic changes in the ovaries (stromal hyperplasia, thecomatosis, interstitial gland, etc.) and endometrial hyperplasia (glandular, atypical, etc.).

[Use of domestic human insulin in patients with type I diabetes mellitus].

Twenty-five patients with type I diabetes mellitus (8 women and 7 men) aged 19 to 54 with a disease duration of 3 to 19 years were treated by Russian semisynthetic short-acting human insulin mono-H. The daily dose varied from 24 to 44 U. The drug therapeutic efficacy was assessed from the terms of attainment of carbohydrate metabolism compensation, glycosylated hemoglobin level, and changes in daily insulin requirement.

[Use of the lipolytic drug mevacor in the treatment of patients with diabetes mellitus].

Stable compensation of diabetes mellitus, including normolipidemia, underlies the therapy of diabetic angiopathies. Mevacor represents a nonactive lactone form of a certain hydroxy acid, a potent inhibitor of endogenous synthesis of cholesterol, conducive to blood cholesterol reduction. The aim of the present study was the assessment of the efficacy of this drug in therapy of patients with diabetes mellitus.

[Early detection of nephroangiopathy in patients with diabetes mellitus and approaches to its correction].

Patients with types I and II diabetes mellitus without the clinical signs of nephroangiopathy were subjected to a comprehensive study of renal function. Using the method for determination of endogenous creatinine clearance (140 patients), radionuclide methods (in 350 patients) and beta-2-microtest (in 130 patients) the impairment of renal filtration function was established in 30.8, 67.

[Use of high-purity insulins in the treatment of patients with diabetes mellitus].

A total of 43 patients aged 16-62 with moderately severe and severe type I diabetes mellitus of 2-27 years duration were observed. The patients were admitted to hospital with decompensation of metabolic processes. In order to achieve compensation monocomponent insulins were administered in an individual dose depending on the levels of glycemia and glucosuria and a scheme of drug administration (2 times continuously, i.

[Urocanic acid content of the epidermis of diabetics].

A degree of a decrease in the content of urocanic acid in washes-off from the skin surface of patients with decompensated diabetes mellitus depends on a period and gravity of disease that may be indicative of disorders of the adenylate cyclase system (or one of its links). Change in the content of urocanic acid in the epidermis of patients with diabetes mellitus treated with the Biostator apparatus, correlates with change in the content of glucagon and blood sugar. The authors discuss a possibility to use the test of urocanic acid determination for the prediction and assessment of the efficacy of therapy of patients with diabetes mellitus.

[Blood glucose, C-peptide and glucagon levels in patients with a labile course of diabetes mellitus during use of the device "Biostator"].

Altogether 100 patients with insulin-dependent diabetes mellitus of a labile course were treated using the Biostator unit (artificial pancreas). The control group (20 persons) was treated with divided doses of insulin within a day. A study was made of glycemia, the range of its deviations, daily glucosuria, diuresis, the basal level of C-peptide and glucagon in the study and control groups prior to and after treatment.

[Proteinuria in diabetics before and after treatment with the Biostator apparatus].

The authors present for the first time their results concerned with the determination of selective proteinurea by disc electrophoresis in polyacrylamide gel in patients with insulin-dependent and insulin-independent diabetes mellitus with and without the clinical signs of kidney affection. A study was made of the uroproteinograms of these patients before and after the correction of carbohydrate metabolism by means of programmed insulin administration. A conclusion was made of a possibility to use the above method for early detection of diabetic nephropathies.

[Clinico-morphological characteristics of diabetic nephroangiopathy in insulin-dependent diabetes mellitus].

The clinicofunctional characteristics of the kidneys were estimated, biopsy of the kidney and skin with an immunohistochemical study was performed and some immunity values considered in 17 patients with type I diabetes mellitus. The study was performed with respect to the degree of expression of the clinical signs of nephropathy including the preclinical stage, too. Three types of morphological changes in the kidney and skin were singled out.

[Indicators of phagocytic activity of neutrophils in patients with diabetes mellitus before and after treatment using the "Biostator"].

Phagocytic function of polymorphonuclear leukocytes was studied in patients with insulin-dependent and insulin-independent diabetes. A total of 52 patients were examined; the patients were mainly young, suffering from insulin-dependent diabetes with or without angiopathies. The leukocyte phagocytic activity was studied with the use of the direct method developed by G.