Blood Level of Nitric Oxide Metabolites and Expression of Genes Regulating NO Synthesis in Early Forms of Non-Alcoholic Fatty Liver Disease.

The levels of NO metabolites in the plasma and mRNA of the NOS3, ATG9B, and NOS2 genes in peripheral blood leukocytes of healthy people and patients with early forms of non-alcoholic fatty liver disease (steatosis and weak activity non-alcoholic steatohepatitis) were studied. In patients with steatohepatitis, the concentration of NO metabolites in the blood and the level of mRNA of the NOS2 gene were higher than in patients with steatosis and healthy people. These differences can be of diagnostic value for distinguishing between steatosis and weak activity steatohepatitis in non-alcoholic fatty liver disease.

Distribution and anthropometric characteristics of Rotterdam criteria-based phenotypic forms of Polycystic ovaries syndrome in Ukraine.

Objective: To explore the distribution of Rotterdam-based PCOS phenotypes and their associations with anthropometric parameters predictive of cardiometabolic risks in Ukrainian referral PCOS women.

Study Design: It was a cross-sectional study conducted by the Ukrainian Society of Gynecological Endocrinology between September 2021 and January 2022 involving 42 clinics in 10 regional centres representing the major geographical parts of Ukraine. Two hundred obstetrician-gynecologists whose practice facilities corresponded to study criteria were committed to entering records of their PCOS patients aged 20-45 years into the uniform data collection forms.

The impact of the war in Ukraine on the reproductive health of female military personnel.

Objective: To explore the link between mental health, physical well-being, and reproductive system pathology in females who suffered war-related concussions.

Methods: A study was conducted at the Kyiv Center of Reproductive and Perinatal Medicine with 715 participants. The group included 457 female military personnel with concussions (211 with post-concussive syndrome (PCS), 246 without), 208 women from occupied/deoccupied areas (103 with PCS, 105 without), and 50 civilians from safe zones.

Self-action effects in hyperbolic metamaterials based on gold nanorods.

Intensive studies of hyperbolic metamaterials (HMMs) are induced by unique optical properties of this type of artificial media associated with their hyperbolic dispersion. Special attention is attracted to the nonlinear optical response of HMMs, which reveals anomalous behavior in definite spectral regions. Third-order nonlinear optical self-action effects that are perspective for applications were analyzed numerically, whereas such experiments have not been performed up to now.

Quantitative Assay of SARS-CoV-2 RNA and Level of Proinflammatory Protein Gene Transcripts in Peripheral Blood Leukocytes after a Novel Coronavirus Infection.

The possibility of finding persistent SARS-CoV-2 viral particles in human peripheral blood leukocytes after a novel coronavirus infection was shown. The results of droplet digital PCR showed that 19 of 24 examined subjects had from 4 to 555 copies of the Nsp4 SARS-CoV-2 gene in 5-6 months after infection. The presence of this transcript in peripheral blood leukocytes was associated with reduced expression of FOXP3 gene and increased level of RORγ gene mRNA.

Physiological and Biochemical Characteristics of Rainbow Trout with Severe, Moderate and Asymptomatic Course of Infection.

This article describes the clinical manifestation of natural infection in rainbow trout (Oncorhynchus mykiss) during an outbreak on a fish farm. (i) Using an integrated approach, we characterized the pathogenesis of vibriosis from the morphological, hematological, and biochemical points of view. The molecular mechanisms associated with the host immune response were investigated using mass spectrometric analysis of trout plasma proteins.

Magneto-optical effects in hyperbolic metamaterials based on ordered arrays of bisegmented gold/nickel nanorods.

Hyperbolic metamaterials (HMM) based on multilayered metal/dielectric films or ordered arrays of metal nanorods in a dielectric matrix are extremely attractive optical materials for manipulating over the parameters of the light flow. One of the most promising tools for tuning the optical properties of metamaterialsis the application of an external magnetic field. However, for the case of HMM based on the ordered arrays of magneto-plasmonic nanostructures, this effect has not been clearly demonstrated until now.

ASSISTED REPRODUCTIVE TECHNOLOGY EFFICIENCY IMPROVEMENT OF TUBAL-PERITONEAL INFERTILITY.

Objective: The aim: This study aims to develop and implement a complex of pregravid preparation based on endogenous melatonin level, development of oxidative stress and violation of apoptosis regulation in women with tubal-peritoneal infertility.

Patients And Methods: Materials and methods: We have examined 100 women of reproductive age, including 65 women with tubal-peritoneal infertility treated by ART. All studied women were defined levels of endogenous melatonin, oxidative status and level of apoptosis in the endometrium and in follicular fluid (infertile women only).

Second-harmonic generation spectroscopy in gold nanorod-based epsilon-near-zero metamaterials.

The interest in hyperbolic metamaterials is fueled by fascinating optical properties exhibited by this class of artificial media. Their optical features originate from hyperbolic dispersion emerging due to the shape anisotropy of the metal-dielectric composite. In this work, we study experimentally and numerically the second-harmonic generation (SHG) in ordered arrays of Au nanorods embedded in porous aluminum oxide.

The Role of Inducible NOS2 Gene Polymorphism in the Development of Essential Arterial Hypertension.

The risk of essential arterial hypertension was assessed in carriers of the NOS2 gene variants (rs1800482 (-954G>C), rs3730017 (C>T)). In subjects carrying C allele (rs1800482), the risk for essential arterial hypertension developing was higher by 1.7 times (OR=1.

DICER and DROSHA gene expression in peripheral mononuclear blood cells from rheumatoid arthritis patients under methotrexate therapy.

Aim: The aim of this research was studying the level of DROSHA and DICER genes expression in peripheral mononuclear blood cells (PBMC) in rheumatoid arthritis (RA) patients under methotrexate therapy.

Materials And Methods: 82 people (from 45 to 70 years) enrolled in this study were divided into 3 groups (the first one - healthy donors (n=33 median age 49.93±1.

Expression of DROSHA and DICER genes in peripheral blood leukocytes in lung sarcoidosis.

Aim: To study the expression level of the genes DROSHA and DICER in peripheral blood leukocytes (PBL) of patients with sarcoidosis of the lungs.

Materials And Methods: The study included 32 patients diagnosed with persistent lung sarcoidosis (mean age 41.56±1.

Analysis of the Association of TNF -238G>A Gene Polymorphism with the Risk of Rheumatoid Arthritis Development in Russian Population in the Republic of Karelia.

We studied association of the TNF gene -238G>A polymorphism (rs361525) with the risk of rheumatoid arthritis development in the Russian population living in the Republic of Karelia. The influence of rs361525 on the development of rheumatoid arthritis was revealed: genetic predisposition to this disease is associated with the presence of GG genotype. The effect of the genotype on the polymorphic locus of -238G>A on TNF mRNA content was revealed.

[Association of FOXP3 gene -3279 C>A polymorphism with the risk of pulmonary sarcoidosis].

Aim: To investigate the association of the polymorphic marker -3279 C>A of the FOXP3 gene with the risk of pulmonary sarcoidosis (PS) and to estimate the transcription level of this gene in the carriers of different genotypes of this polymorphic marker.

Subjects And Methods: The investigation included 99 patients of Russian ethnicity (mean age, 45.41±1.

[Efficacy of sulodexide in treatment of chronic venous insufficiency. Results of the ACCORD trial].

Objective: The purpose of our study was to evaluate both clinical and laboratory efficacy of sulodexide given at a daily dose of 500 lipasemic units (LSU) in patients presenting with class C3-C4 chronic venous insufficiency (CVI) according to the CEAP classification.

Patients And Methods: The study included a total of 25 patients diagnosed with C3-C4 CVI and prescribed to receive sulodexide at a daily dose of 500 LSU for 90 days. Efficacy was comprehensively controlled by the following tools: the disease-specific Chronic Venous Insufficiency Quality of Life Questionnaire (CIVIQ), visual-analogue methods of assessment separate symptoms; the Venous Clinical Severity Score (VCSS), as well as ultrasonographic determination of the thickness of subcutaneous fat and crural fascia.

[Analysis of an association of TNF -308G>A polymorphism with a risk for pulmonary sarcoidosis in the Russian population of the Republic of Karelia].

Aim: To analyze an association of TNF -308G>A polymorphism with a risk for pulmonary sarcoidosis (PS) in the Russian population of the Republic of Kareli.

Subjects And Methods: 84 patients with persistent PS and 96 donors without clinical manifestations of this disease (a control group) were examined. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis was used to identify alleles and genotypes by the marker of TNF -308G>A polymorphism.

The level of cytokines and expression of caspase genes in rheumatoid arthritis.

The level of TNFα and IL6 in the blood plasma of patients with rheumatoid arthritis (RA) who received antiinflammatory therapy with methotrexate (MT) was significantly lower than in the patients without MT treatment. The level of caspase 6 and 9 gene transcripts in peripheral blood lymphocytes in patients with rheumatoid arthritic diagnosed for the first time and in patients with MT treatment were not significantly different. At the same time, the level of caspase 3 mRNA expression was significantly higher in the cells of the RA patients with MT therapy compared to the patients without MT therapy.

[Identification of the functional activity of synthetic polyamine analogues using a biotest system based on highly proliferating cultured human cells].

A new biotest system was developed based on highly proliferating human cell cultures (lines LNCaP and PC-3). With the help of this system, two known synthetic polyamines--alpha-difluoromethylornithine (DFMO) and methylglioxalbis(guanylhydrason) (MGBG)--as well as four new synthetic analogues difenyl containing amines (DFCA-1-DFCA-4) with molecular weights of 725.5 (DFCA-1), 755.

[The assessment of prognosis of carotid endarterectomy by cliniсal-mathematical method].

We examined 127 patients with atherosclerotic stenosis of carotid arteries who underwent carotid endarterectomy (CEAE). In the most of patients (85%), CEAE slowed the progression of chronic cerebral ischemia leading to the stabilization or improvement of neurologic and/or neuropsychological status. The analysis of the data allowed to suggest a mathematical model for predicting the outcome of the surgery.

[Liver regeneration after its mechanical injury in rats receiving biologically active substances "Trepel" and "Suvar"].

The effect of biologically active substances (BAS) "Trepel" and "Suvar" on liver regeneration 1-30 days after its mechanical injury was studied using histological and morphometric methods in 110 rat pups aged 18 days. The control group comprised 90 animals that received no treatment following liver injury. It was shown that both BAS studied inhibited the inflammatory reaction around the injury focus, suppressed collagenogenesis and activated hepatocyte proliferation.

[Evaluating regional system of occupational medical service organization].

The article covers analysis of regional occupational pathology service designed for primary medical and sanitary care for workers and specialized care in occupational pathology center. The authors proved cooperation between occupational pathology center and Occupational Pathology department of the Medical University to be optimal.

[Genetic diversity of insular natural populations of Festuca pratensis Huds.: RAPD analysis].

In natural populations of Festuca pratensis Huds. from the islands of Onega Lake, the level of genetic diversity was evaluated. In three populations variability of 64 RAPD loci was tested.