Microphysiological model reveals the promise of memory-like natural killer cell immunotherapy for HIV cancer.

Numerous studies are exploring the use of cell adoptive therapies to treat hematological malignancies as well as solid tumors. However, there are numerous factors that dampen the immune response, including viruses like human immunodeficiency virus. In this study, we leverage human-derived microphysiological models to reverse-engineer the HIV-immune system interaction and evaluate the potential of memory-like natural killer cells for HIV head and neck cancer, one of the most common tumors in patients living with human immunodeficiency virus.

Correction: Pakuła et al. Deciphering the Molecular Mechanism of Spontaneous Senescence in Primary Epithelial Ovarian Cancer Cells. 2020, , 296.

In the original publication [...

Autofluorescence lifetime imaging classifies human lymphocyte activation and subtype.

New non-destructive tools are needed to reliably assess lymphocyte function for immune profiling and adoptive cell therapy. Optical metabolic imaging (OMI) is a label-free method that measures the autofluorescence intensity and lifetime of metabolic cofactors NAD(P)H and FAD to quantify metabolism at a single-cell level. Here, we investigate whether OMI can resolve metabolic changes between human quiescent versus IL4/CD40 activated B cells and IL12/IL15/IL18 activated memory-like NK cells.

Initial non-amnestic symptoms relate to faster rate of functional and cognitive decline compared to amnestic symptoms in neuropathologically confirmed dementias.

Introduction: The relationship between initial cognitive symptoms and subsequent rate of clinical decline is important in clinical care and the design of dementia clinical trials.

Methods: This retrospective longitudinal, autopsy-confirmed, cohort study among 2426 participants in the National Alzheimer's Coordinating Center database included Alzheimer's disease (AD) pathology, n = 1187; Lewy body pathology (LBP), n = 331; and mixed pathology (AD-LBP), n = 904. The predominant initial cognitive symptom was assessed clinically.

The Mental Health of Emerging Adults: Hostile Home Environments and COVID-19.

Purpose: Due to shifts in societal and educational expectations alongside the COVID-19 pandemic, many emerging adults live with their family of origin for extended periods of time. Little is known about patterns of parent-perpetrated maltreatment in emerging adulthood. Therefore, this study evaluates the relation between forms of parent-perpetrated maltreatment, including economic abuse, and COVID stress, on symptoms of depression, anxiety, and traumatic stress.

Insulin sensing by astrocytes is critical for normal thermogenesis and body temperature regulation.

The important role of astrocytes in the central control of energy balance and glucose homeostasis has recently been recognized. Changes in thermoregulation can lead to metabolic dysregulation, but the role of astrocytes in this process is not yet clear. Therefore, we generated mice congenitally lacking insulin receptors (Ir) in astrocytes (IrKOGFAP mice) to investigate the involvement of astrocyte insulin signaling.

Migraine and Ischemic Stroke in Women. A Narrative Review.

Objective/background: Migraine is associated with ischemic stroke. Women are 3-fold as likely as men to have migraine, and high estrogen states increase the risk of migraine with aura (MWA), venous thromboembolism (VTE), and of stroke. We review the epidemiological and mechanistic evidence of the migraine-stroke relationship and its risk factors, with a focus on women and conditions that exclusively or predominantly affect them.

Enzymatically derived sunflower protein hydrolysate and peptides inhibit NFκB and promote monocyte differentiation to a dendritic cell phenotype.

Enzymatic hydrolysis of proteins produces bioactive peptides that have the potential to provide health benefits. This study examined the inflammatory- and immune-modulating properties of a flavourzyme-derived sunflower protein hydrolysate (SPH) and peptides. The SPH was fractionated into <1, 1-3, 3-5, and >5 kDa peptides by membrane ultrafiltration.

GROWTH AND SHRINKAGE PRE AND POST TSUNAMI IN FUKUSHIMA PREFECTURE, JAPAN.

Depopulation is a severe problem in many urban areas globally. Massive population migrations can occur due to relocation after natural disasters and significantly change the demographic composition of regions and cities. The 2011 Great Tsunami in Japan resulted in a combined total of deaths and missing persons of more than 24,500.

Deciphering the Molecular Mechanism of Spontaneous Senescence in Primary Epithelial Ovarian Cancer Cells.

Spontaneous senescence of cancer cells remains a puzzling and poorly understood phenomenon. Here we comprehensively characterize this process in primary epithelial ovarian cancer cells (pEOCs). Analysis of tumors from ovarian cancer patients showed an abundance of senescent cells in vivo.

Serum from patients with chronic obstructive pulmonary disease promotes proangiogenic behavior of the vascular endothelium.

Objective: It has been documented that COPD is a risk factor for lung cancer. In COPD patients, changes in lung angiogenesis - a critical process in the development of lung cancer - have been poorly investigated. We aimed to determine whether serum from COPD patients could promote the proangiogenic capabilities of endothelial cells in vitro.

Polymorphisms in microRNA target sites modulate risk of lymphoblastic and myeloid leukemias and affect microRNA binding.

Background: MicroRNA dysregulation is a common event in leukemia. Polymorphisms in microRNA-binding sites (miRSNPs) in target genes may alter the strength of microRNA interaction with target transcripts thereby affecting protein levels. In this study we aimed at identifying miRSNPs associated with leukemia risk and assessing impact of these miRSNPs on miRNA binding to target transcripts.

[Cytogenetic analysis of secondary myelodysplastic syndrome after rhabdomysarcoma treatment--case report].

Rhabdomyosarcoma (RMS) is the most common pediatric soft tissue sarcoma. Alveolar subtype of RMS is characterized by very poor prognosis and intensive chemotherapy, radiotherapy and surgery often failed. Frequently a secondary malignant neoplasms has been found in patients treated for primary cancer.

Juvenile xanthogranuloma with clonal proliferation in the bone marrow.

The triple association between juvenile xanthogranuloma (JXG), juvenile myelomonocytic leukemia and neurofibromatosis was described in literature in about 20 cases. In this paper, the case of an 11-month-old infant boy with a disseminated JXG with unusual cytogenetic representation in the bone marrow was reported. Neurofibromatosis and juvenile myelomonocytic leukemia were excluded, just the same as other leukemias.

Water immersion does not alter the minimal erythema dose.

To determine the water resistance of a sunscreen, the COLIPA method requires the determination of two minimal erythema doses (MEDs): a static MED (MED(US)) and a wet MED (MED(UW)) (1). The MED(US) is used in calculating the static SPF; the MED(UW) is used in calculating the SPF after water immersion. Herein, we report that in the 107 subjects examined, the mean MED(US) (21.

FLT3 internal tandem duplication and FLT3-D835 mutation in 80 AML patients categorized into cytogenetic risk groups.

Background: Acute myeloid leukemia (AML) is a clonal disorder characterized by various genetic abnormalities and variable response to treatment. About 50% of patients with AML have no cytogenetic aberrations, presenting normal karyotype, and are categorized in the intermediate risk group. In this group detection of FLT3 mutations move a patient from the intermediate to the adverse risk group.

Development of porcine transgenic nuclear-transferred embryos derived from fibroblast cells transfected by the novel technique of nucleofection or standard lipofection.

The aim of our study was to determine the in vitro developmental potential of porcine nuclear-transferred (NT) embryos that had been reconstructed with Tg(pWAPhGH-GFPBsd) transgene-expressing fibroblast cells. The gene construct was introduced into fibroblast cells by the novel method of nucleofection or standard lipofection. NT oocytes derived from foetal and adult dermal fibroblast cells were stimulated by either simultaneous fusion and electrical activation (Groups IA and IB) or sequential electrical and chemical activation (Groups IIA and IIB).

Improved measurements of CP-violating asymmetry amplitudes in B0-->pi+pi- decays.

We present updated measurements of the CP-violating parameters Spipi and Cpipi in B0-->pi+pi- decays. Using a sample of 227x10(6) Upsilon(4S)-->BB decays collected with the BABAR detector at the PEP-II asymmetric-energy e(+)e(-) collider at SLAC, we observe 467+/-33 signal decays and measure Spipi=-0.30+/-0.

Production and decay of xi(c)(0) at BABAR.

Using 116.1 fb(-1) of data collected by the BABAR detector, we present an analysis of xi(c)(0) production in B decays and from the cc continuum, with the xi(c)(0) decaying into omega- K+ and xi- pi+ final states. We measure the ratio of branching fractions B(xi(c)(0) --> omega- K+)/B(xi(c)(0) --> xi- pi+) spectrum is measured on and 40 MeV below the upsilon(4S) resonance.

Measurement of branching fractions and charge asymmetries in B+ decays to eta pi+, eta K+, eta rho+, and eta' pi+, and search for B0 decays to eta K0 and eta omega.

We present measurements of branching fractions and charge asymmetries for six B-meson decay modes with an eta or eta(') meson in the final state. The data sample corresponds to 232 x 10(6) BB pairs collected with the BABAR detector at the PEP-II asymmetric-energy e(+)e(-) B Factory at SLAC. We measure the branching fractions (in units of 10(-6)): B(B+ -->eta pi(+))=5.

Measurement of the Cabibbo-Kobayashi-Maskawa angle gamma in B+/--->D*K+/- decays with a Dalitz analysis of D-->K0(S)pi-pi+.

We report on a measurement of the Cabibbo-Kobayashi-Maskawa CP-violating phase gamma through a Dalitz analysis of neutral D decays to K0(S)pi-pi+ in the processes B+/- -->D*K+/-, D*-->Dpi0, Dgamma. Using a sample of 227 x 10(6) BB pairs collected by the BABAR detector, we measure the amplitude ratios r(B)=0.12+/-0.

Search for strange-pentaquark production in e+e- annihilation at sqrt[s] = 10.58 GeV.

We search for strange-pentaquark states that have been previously reported by other experiments--the Theta (1540)(+), Xi(5)(1860)(--), and Xi(5)(1860)(0)--in 123 fb(-1) of data recorded with the BABAR detector at the PEP-II e(+)e(-) storage ring. We find no evidence for these states and set 95% confidence level upper limits on the number of Theta(1540)(+) and Xi(5)(1860)(--) pentaquarks produced per e(+)e(-) annihilation into qq and Gamma(4S) decay. For qq events the Theta(1540)(+) [Xi(5)(1860)(--)] limit is about 8 [4] times lower than the rates measured for ordinary baryons of similar mass.

Measurement of the branching fraction of Gamma(4S) --> B0B0.

We report the first measurement of the branching fraction f(00) for Gamma(4S) --> B(0)B(0). The data sample consists of 81.7 fb(-1) collected at the Gamma(4S) resonance with the BABAR detector at the SLAC PEP-II asymmetric-energy e(+)e(-) storage ring.