A Method to Determine Xenobiotic Acetylation Rate by Taq SNP rs1495741.

Drug acetylation plays an important role in the medical practice. Modern methods of acetylation phenotype prediction are based on genotyping of polymorphisms in the second exon of the gene NAT2. Some disadvantages of these methods limit their application in the clinical practice.

A Method for Evaluation of the Level of Circulating Mitochondrial DNA by ND1 and ND2 Genes.

The measurement of the level of mitochondrial DNA (mtDNA) in the blood is a difficult problem due to high variability of mitochondrial genes, deletions in the mitochondrial genome in some pathological conditions, different sources of mtDNA into the bloodstream (mtDNA from tissues, from blood cells, etc.). We designed primers and TaqMan probes for highly conserved regions of the ND1 and ND2 genes outside the mitochondrial deletions "hot zones".

Search for Effective Serum Tumor Markers for Early Diagnosis of Hepatocellular Carcinoma Associated with Hepatitis C.

Unlabelled: was to identify the most effective serum tumor markers for early diagnosis of hepatocellular carcinoma based on the combination of diagnostic characteristics and correlations.

Materials And Methods: There were observed 55 patients with chronic hepatitis C in the stage of liver cirrhosis with a verified diagnosis of hepatocellular carcinoma. The control group consisted of 55 patients with chronic hepatitis C at the stage of liver cirrhosis without hepatocellular carcinoma, comparable to the experimental group in terms of basic clinical profile.

Clinical and Experimental Evaluation of Diagnostic Significance of Alpha-Fetoprotein and Osteopontin at the Early Stage of Hepatocellular Cancer.

We evaluated the possibility of using an experimental model of hepatocellular carcinoma to study oncomarkers of primary liver cancer and compared the diagnostic efficacy of alpha-fetoprotein and osteopontin in the experiment and in clinical practice. Experimental studies were performed on a model of hepatocellular carcinoma induced by administration of diethyl nitrosamine to Fisher-344 rats. In addition, the levels of α-fetoprotein and osteopontin were determined in 35 patients with hepatocellular carcinoma detected at stages I-II according to TNM classification.

[Assessment of external risk factors of hepatocellular cancer development and markers of genetic predisposition to its development in the ethnic group of yakut - men].

Aim: To establish the main external and genetically determined risk factors for the development of hepatocellular cancer in the ethnic group of male Yakuts living in the Republic of Sakha (Yakutia) [RS (Y)] in the epidemiologically unfavorable conditions of the incidence of viral hepatitis.

Materials And Methods: A total of 97 male Yakuts were examined, including 44 people diagnosed with hepatocellular cancer and 53 people diagnosed with chronic viral hepatitis. HCC risk factors were identified by analyzing medical records and questioning patients.

[Application of alpha-fetoprotein and osteopontin combination for early diagnosis of hepatocellular carcinoma associated with hepatitis C.].

Liver cirrhosis in the outcome of hepatitis C is the leading cause of hepatocellular carcinoma (HCC) in the world. Early diagnosis and timely treatment of HCC are important for reducing mortality and increasing life expectancy of patients with hepatocellular carcinoma. To assess the risk of HCC, the definition of alpha-fetoprotein (AFP) in the blood is most widely used, but low sensitivity limits its diagnostic value.

Population Polymorphism of IFNL3 and IFNL4 Genes of Type 3 Interferon Associated with Spontaneous Clearance of Hepatitis C Virus in Representatives of Caucasian and Mongoloid Races.

The frequency of single nucleotide polymorphisms of IFNL3 gene (rsl12979860 and rs8099917) and dinucleotide polymorphism of IFNL4 gene (ss469415590) were estimated in healthy inhabitants of Mongolia and Irkutsk regions taking into account their races. Population and genetic studies were performed in 1520 conventionally healthy volunteers (blood donors), representatives of Caucasian and Mongoloid races. Significant race-related differences in the incidence of IFNL3 and IFNL4 gene polymorphisms associated with spontaneous clearance of hepatitis C virus were found in healthy volunteers.

Association Equilibrium for Cross-Associating Chains in a Good Solvent: Crowding and Other Nonideality Effects.

Association equilibrium has been studied by molecular dynamics (MD) for mixtures of cross-associating molecules (n-decamer+p-dimer and n-decamer+p-decamer) in a good solvent. Each monomer of n-decamers carries an associative site (n-sticker); each molecule of the second component contains two terminal associative sites (p-stickers). To model the univalent association between the n-sticker and the p-sticker, a technique based on introduction of dummy atoms has been used.

[EPIDEMIOLOGIC MANIFESTATIONS OF VIRAL HEPATITIS C IN REPRESENTATIVES OF CAUCASIAN AND MONGOLOID RACES].

Aim: Present comparative epidemiologic characteristics of viral hepatitis C in Mongolia and Irkutsk Region taking into account racial origin of the studied populations.

Materials And Methods: The studies were carried out in 2009-014 on the territory of Irkutsk Region in Mongolia. Prevalence of viral hepatitis based on serological monitoring, virus RNA detection, risk factors, change in structure of circulating genotypes, hepatocellular carcinoma morbidity were studied.

[Sanitary-Epidemiological Status of Siberian Population (Medico-Demographical and Epidemiological Characteristics)].

Review on the problem of sanitary-epidemiological welfare of the population in the Siberian Federal District (SFD) was conducted based on literature data and authors own research in the period of 2002−2014. Authors provided broad information on the health and demographic and epidemiological characteristics of SFD population. SFD in comparison with other regions of the Russian Federation overcomes one of the most adverse situations including mortality rates from external causes.

[The early diagnosis of neurodegenerative changes in multiple sclerosis and clinically isolated syndrome based on optical coherence tomography of the retina].

Aim: To study signs of neurodegeneration in the retina in multiple sclerosis.

Material And Methods: We studied the retinal nerve fiber layer (RNFL) in 265 (524 eyes) patients.

Results And Conclusion: We identified a reliable thinning of RNFL of both eyes in patients with relapsing-remitting multiple sclerosis (MS).

[Diagnosis of human lifetime height using foot length in forensic medical examination of corpse fragments].

This review of domestic and foreign literature concerns methods for the rough estimation of human height based on the results of foot measurement. The efficiency of all these methods appears to be very low since they yield differing and mostly unreliable information. Their main drawback is attributable to the poor dependence between the foot length and the height.

[Results of using biocoating by means of the amniotic sac. Communication 1. Keratoplasty with graft biocoating].

To protect a corneal graft at keratoplasty from tear moisture hyperhydration of the de-epithelized surface and from the negative exposure to contact with the eyelid mucosa in order to enhance surgical efficiency, the authors have developed a few biocoating technologies that involve amnionic sac placement on the graft, with its fixation on the eye of a recipient, by applying a full-thickness and layered corneal disc autologous flap or a ring-shaped allogenic corneoscleral one. Fifty-two patients (55 eyes) with primary (n=5) and secondary (n=33) corneal dystrophy and leukomas (n=14) were operated on. Penetrating keratoplasty and layer-by-layer grafting were performed in 38 (40 eyes) and 14 (15 eyes) patients, respectively.

[Current aspects of the clinical picture of tick-borne encephalitis].

Based on the long-term follow-up of 655 patients with different forms of tick-borne encephalitis (TBE), the authors describe its present clinical characteristics in the Baikal natural focus. They have established the preponderance of the meningeal form of the infection and the relatively severe course with residual phenomena and a fatal outcome in patients with the poliomyelitic form of TBE.

[Glutathione system in erythrocytes and plasma in viral hepatitis].

In all 5 acute (AVHs) and chronic viral hepatites (CVHs) there was the increase of erythrocyte activities of glutathione peroxidase (GPx) and glutathione reductase (GR), and the decrerase in GSH concentration. In blood plasma there was accumulation of GPx, glutathione S-transferase (GST) and y-glutamyl transferase (gammaGT). GSH and GR increased in plasma only in AVHs.

[Diagnosis and differential treatment of acute pancreatitis of biliary etiology].

The experience in examination and treatment of 208 patients with acute pancreatitis of biliary etiology was analyzed. Complex endoscopic treatment was carried out in 88% patients. If there are indications, it is reasonable to perform retrograde pancreatocholangiography (RPCG) and endoscopic papillosphincterotomy (EPST) during surgery in patients with concomitant enzymatic ascites-peritonitis.

[Comparative analysis of different methods in the treatment of patients with acute calculous cholecystitis and choledocholithiasis].

In the patients with different forms of cholelithiasis 1988 operations were performed, 597 of them - on account of acute calculous cholecystitis. In 118 (19%) patients with acute calculous cholecystitis choledocholithiasis was diagnosed. All the patients were divided into two groups.

[Surgical treatment of creeping infections of the flexor foot tendons in diabetic patients].

Unlabelled: The creeping infections of the flexor foot tendons are a common reason for amputation of limbs in diabetic patients. Usually the process starts from the fingers and spreads through their long flexors.

Methods: Of the total of 149 operated patients, 49 underwent selective removal of the m.

[Retrospective analysis of the results of surgical treatment of 407 patients with differential thyroid gland cancer over a period of 23 years].

Over a period of 23 years (1980-2002) 407 cases of differential thyroid gland cancer were operated, monitored and analyzed at the Endocrine Surgery Clinic of Alexandrovska University Hospital. Of them 71.7% were monitored over a period of more than 10 years (up to 23 years), and 39.

[Impact of Yersinia pseudotuberculosis on the in vitro production of cytokines by whole blood cells of donors].

The impact of two plasmid (47, 82 MD), single plasmid (47 MD) and non plasmid Y. pseudotuberculosis strains, Y. enterocolitica (47 MD) as well as Y.

[Relations of acid-base balance and gastric secretion of hydrogen carbonate ions in patients with peptic ulcer].

Aim: To study acid-base balance (ABB) of the organism, gastric secretion of hydrocarbonate (HCO3-) in ulcer patients and to examine their relationships.

Material And Methods: ABB in capillary or venous blood before and after losec and famotidine treatment and gastric secretion of HCO3- were studied in 72 patients with ulcer.

Results: It was found that patients with gastroduodenal ulcer have metabolic, less frequently hypercapnic acidosis with suppressed gastric secretion of HCO3-.