Rapid detection of infectious flacherie virus of the silkworm, Bombyx mori, using RT-PCR and nested PCR.

In this study, a method for detection of an ssRNA viral pathogen that causes viral flacherie in the silkworm, Bombyx mori (L.) (Lepidoptera: Bombycidae), was used for the detection of B. mori infectious flacherie virus (BmIFV).

Effective solubilization procedure for analysis of silkworm hemolymph proteins by two-dimensional gel electrophoresis.

The outstanding capability of two-dimensional gel electrophoresis in separating all types of proteins basically depends on the efficiency of sample preparation. Sample preparation is one of the most critical steps in two-dimensional gel electrophoresis. Unfortunately, due to severe solubility, resolution of protein on gel is usually hampered, and thus, analysis remains a difficult task.