[Mycological and entomological methods for estimation of the time since death in forensic medicine].

The objective of the present paper was to summarize the results of experimental studies and expert estimation carried out by the authors to survey the process of cadaver biodecomposition under different conditions with special reference to the characteristics of soil biota at different burial sites. Criteria have been developed for the estimation of prescription of death coming and the identification of biotic communities of the corpse buried in the soil, saline and fresh water.

[Effect of Acholeplasma laidlawii var. granulum on lectin activity of sugar beet calluses].

The dynamics of lectin activity of sugar beet calluses infected by mollicute Acholeplasma laidlawii var granulum str. 118 was studied. Activity of acid-soluble lectins of sugar beet cell cultures increased 4.

[Mono- and polyvalent synthetic sialosides as inhibitors of Mycoplasma pneumoniae adhesiveness].

Nine mono- and polyvalent sialosides as substances inhibiting adhesive properties of mycoplasma Mycoplasma pneumoniae--the agent of human atypical pneumonia have been studied in the reaction of hemagglutination (RHA) using solid-phase variant of ELISA-test ("sandwich"--variant), which is based on the competition for specific binding in RHA between the studied syalosides and sialylglycoproteins of fetuin conjugate with horseradish peroxidase. Of seven polymers--P alpha.12.

[The inhibition of Mycoplasma pneumoniae adhesion in a fetuin test system by synthetic analogs and polymeric forms of neuraminic acid].

Eight glycosides and structural analogues of neuraminic acid as well as eight polymeric forms of N-acetyl neuraminic acid have been studied for their inhibitory effect on adhesion of Mycoplasma pneumoniae. Maximum inhibiting effect among low-molecular compounds was manifested by 2-->3 sialyllactose which, being used in concentrations 5.0 and 10.

[The inhibitory action of 6-azacytidine on Mollicutes and its proposed mechanism].

6-Azacytidine (6-AC) is shown to have an inhibitory effect on the Mollicutes of the different systematic position. The growth of type strains of Mollicutes (Acholeplasma laidlawii PG-8, Mycoplasma pneumoniae FH and M. fermentans PG-18) completely ceased in the nutrient medium at concentration of the above substance in it within the range of 125-250 micrograms/ml.

[The effect of physicochemical factors on the activity of DNA-dependent DNA polymerases in Acholeplasma laidlawii PG-8].

The biological and physico-chemical properties of DNA-dependent DNA-polymerases of Acholeplasma laidlawii PG-8 have been studied. The optimal parameters of maximal enzymatic activity are determined. It is stated that N-ethylmaleimide in concentration of 1 mM activated DNA-polymerase I by 52%, whereas DNA-polymerase II with reagent concentration of 0.

[A trial of cultivating Rickettsiella phytoseiuli on the SM IMV-72 medium used for growing phytopathogenic mycoplasmas].

The work presents data on growing Rickettsiella phytoseiuli in the SM IMV-72 medium which is used for cultivation of phytopathogenic mycoplasmas. Rickettsiella was observed till the 66th day in the primary cultures and till the 16th day after the first passage. Binary division of the cells has been found only in the primary cultures; a complex cycle of the Rickettsiella development ceased at the stage of formation of the crystal-forming cells.

[The isolation and partial purification of 2 DNA-dependent DNA polymerases from Acholeplasma laidlawii PG-8].

Two forms of DNA-dependent DNA-polymerase have been isolated and partially purified from the limited amount of biomass of cells Acholeplasma laidlawii PG-8, a typical representative of genus Acholeplasmataceae, as a result of successive chromatography on the columns with DEAE-cellulose DE-52 and Green A-sepharose. The first form of DNA-polymerase is eluted from the ion-exchange column with NaCl concentration of 0.1 M from the column with Green A-sepharose of 0.

[Choice of the optimal method of numerical analysis in taxonometric study of mollicutes based on electrophoresis of their proteins].

Different procedures of numerical analysis are evaluated to select the most convenient of them for processing the results of mycoplasm protein electrophoresis in polyacrylamide gel (EPAAG) when solving problems of their taxonomy and systematics. This aim is satisfied most of all by the method of Sokal which permits revealing relations at the rank of species, genus and higher taxons. This method as well as the approaches developed by the authors for transforming results of EPAAG of proteins into the numerical form are recommended to improve the systematics of mollicutes with application of computers.

[Therapeutic efficacy of trimecaine in cardiac rhythm disorders and ways of improving it].

The efficacy of antiarrhythmic therapy with trimecain in 5 different regimens was studied in 87 patients, in 146 episodes of various disorders of the cardiac rhythm. On the basis of clinical and experimental (on 14 dogs with ventricular arrhythmia) studies, different schemes of trimecain treatment are compared. It was shown that combination of the intravenous and the subsequent intramuscular administration of trimecain to treat ventricular extrasystole complicating the course of acute myocardial infarction is as effective as prolonged instillation but exerts a longer action and is better tolerated by patients.