Antimicrobial Effect of Imipenem-Functionalized FeO Nanoparticles on Producing Metallo β-lactamases.

Background: Resistant strains of to imipenem was medical treatment problem, especially in burnt units of hospitals.

Objectives: This study was conducted to evaluate the antimicrobial effect of FeO nanoparticles alone and functionalized with imipenem on starins producing metallo β-lactamases (MBL).

Materials And Methods: A disk diffusion method was used to isolate a clinical producing Metallo β-lactamases with imipenem resistance.

Detection and discrimination of two Brucella species by multiplex real-time PCR and high-resolution melt analysis curve from human blood and comparison of results using RFLP.

Objectives: Rapid and accurate detection of Brucella abortus and Brucella melitensis from clinical samples is so important because antibiotic treatment has major side effects. This study reveals a new method in detection of clinical samples of brucellosis using real-time PCR and high-resolution melt (HRM) curve analysis.

Materials And Methods: 160 brucellosis suspicious samples with more than 1/80 serum antibody titers were collected and the results were compared with the RFLP method.