Serotonin-Related Gene Variants in Patients with Irritable Bowel Syndrome and Depressive or Anxiety Disorders.

Aim: To assess the association of six polymorphisms in serotonin-related genes with depressive or anxiety disorders in patients with irritable bowel syndrome (IBS).

Methods: The lifetime prevalence of depressive and anxiety disorders was assessed in 95 IBS patients (85% women) using the Munich version of the Composite International Diagnostic Interview (CIDI). IBS was diagnosed according to the Rome III criteria.

[Efficiency of three commercial kits dedicated to DNA and RNA isolation from various clinical and forensic materials using the Janus automated workstation].

Isolation of genetic material is a crucial stage in molecular biology. Increasing needs for DNA analysis cause continuous improving of genetic material isolation methods toward higher accuracy and output. Automatization in molecular biology is widely seen, especially in clinical and forensic medicine.

Single nucleotide polymorphisms within functional regions of genes implicated in insulin action and association with the insulin resistant phenotype.

Type 2 diabetes and insulin resistance (IR) are characterized by severe anomalies in genes expression rate including genes involved in insulin signal transduction. Post-transcriptional regulation of gene expression is crucial for many physiological processes and is mediated mainly by untranslated region (UTR). Present study concentrated on the search for correlation between single nucleotides polymorphisms in UTRs of the INSR, PIK3R1, PTPN1, and SLC2A4 genes and IR.