Optimization of Se- and Zn-Enriched Mycelium of (Berk.) Pegler as a Dietary Supplement with Immunostimulatory Activity.

Mycelial cultures of , an edible and medicinal mushroom, have been used in our previous research to obtain selenium-containing immunomodulatory preparations. Our current attempts to obtain a new preparation containing both selenium and zinc, two micronutrients necessary for the functioning of the immune system, extended our interest in the simultaneous accumulation of these elements by mycelia growing in media enriched with selenite and zinc(II) ions. Subsequently, we have studied the effects of new mycelium water extracts with different concentrations of selenium and zinc on the activation of T cell fraction in human peripheral blood mononuclear cells (PBMCs).

Synthesis of a new scaffold: the 7H,8H-pyrimido[1,6-b]pyridazin-6,8-dione nucleus.

This paper describes a modified method of preparation of a number of alpha-aryl-alpha-(pyridazin-3-yl)-acetonitriles via the C-arylation reaction of the corresponding carbanionsof phenylacetonitriles using 3-chloropyridazine derivatives. KOH and DMSO were used inthe deprotonation process, which made the reaction very simple and safe to perform.Nitriles were obtained in the hydrolysis reaction to the corresponding alpha-aryl-alpha-(pyridazin-3-yl)-acetamide derivatives, which were next subjected to cyclization to afford the finalproducts.

Accumulation of zinc by the lentinus edodes (Berk.) mycelium cultivated in submerged culture.

To obtain rich in organic forms of zinc extracts of Lentinus edodes, possessing putative higher immunostimulating properties than actually used, mycelia were cultivated in zinc(II) enriched media. Culture media were enriched in zinc in concentration ranging from 0 to 90 microg mL(-1), added to the medium before inoculation. Total zinc concentration in submerged cultivated mycelial biomass has been determined by the use of atomic absorption spectroscopy method (AAS).