Modification of plasma membrane NADPH oxidase activity in cucumber seedling roots in response to cadmium stress.

The aim of this study was to investigate the effect of cadmium on plasma membrane (PM) NADPH oxidase activity in cucumber roots. Plants were treated with cadmium for 1, 3 or 6 days. Some of the plants after 3-day exposure to cadmium were transferred to a medium without the heavy metal for the next 3 days.

Transcriptional regulation of the V-ATPase subunit c and V-PPase isoforms in Cucumis sativus under heavy metal stress.

Two electrogenic proton pumps, vacuolar H(+) transporting ATPase (V-ATPase, EC 3.6.3.

Modification of plasma membrane proton pumps in cucumber roots as an adaptation mechanism to salt stress.

The effect of salt stress (50mM NaCl) on modification of plasma membrane (PM) H(+)-ATPase (EC 3.6.3.

Abscisic acid and hydrogen peroxide induce modification of plasma membrane H(+)-ATPase from Cucumis sativus L. roots under heat shock.

We examined the effect of heat shock (HS), for 2 h at 48°C, on plasma membrane H(+)-ATPase (PM-H(+)-ATPase) measured as the hydrolytic and H(+)-pumping activity. Some of the plants were transferred after 2 h HS to control temperature for another 24 h, as post-stressed (PS) plants. A significant increase of PM-H(+)-ATPase in plants subjected to HS was observed.

Mechanism of Cd and Cu action on the tonoplast proton pumps in cucumber roots.

The effect of Cd and Cu on the tonoplast proton pumps, V-ATPase (EC 3.6.3.

Different effect of cadmium and copper on H+-ATPase activity in plasma membrane vesicles from Cucumis sativus roots.

The effect of heavy metals on plasma membrane (PM) H(+)-ATPase (EC 3.6.3.

Response of plasma membrane H(+)-ATPase to low temperature in cucumber roots.

The effect of low temperature (LT, 10°C) on modification of plasma membrane (PM) H(+)-ATPase (EC 3.6.3.

Differential regulation of vacuolar H+-ATPase and H+-PPase in Cucumis sativus roots by zinc and nickel.

Zinc and nickel, as micronutrients, are essential for all organisms. We investigated the effect of 10 and 100 μM Zn and Ni on two tonoplast proton pumps, vacuolar H+-ATPase (V-ATPase) (EC 3.6.

Different responses of tonoplast proton pumps in cucumber roots to cadmium and copper.

Cadmium (Cd) and copper (Cu) effects on the two tonoplast proton pumps were compared in cucumber roots. Different alterations of vacuolar H+ transporting ATPase (V-ATPase) (EC 3.6.

The role of polyamines in the regulation of the plasma membrane and the tonoplast proton pumps under salt stress.

Polyamine content (PAs) often changes in response to abiotic stresses. It was shown that the accumulation of PAs decreased in roots treated for 24h with 200 mM NaCl. The role of polyamines (putrescine - PUT, spermidine - SPD and spermine - SPM) in the modification of the plasma membrane(PM) H(+)-ATPase (EC 3.

Response of plasma membrane H+-ATPase to heavy metal stress in Cucumis sativus roots.

The effect of heavy metals on the modification of plasma membrane H(+)-ATPase (EC 3.6.3.

Comparison of heavy metal effect on the proton pumps of plasma membrane and tonoplast in cucumber root cells.

The effects of 10 microM cadmium, copper and nickel on the activities of vacuolar membrane and plasma membrane (PM) ATP-dependent proton pumps was investigated in Cucumis sativus L. root cells. It was demonstrated that vacuolar H+-ATPase (EC 3.

Modification of plasma membrane and vacuolar H+ -ATPases in response to NaCL and ABA.

The role of ABA in the modification of membrane proton pump activity in cucumber roots which had been stressed for 24h with 200 mmol/dm(3) NaCl was investigated. It was shown that treatment of plants with salt distinctly increased the activity of the plasma membrane H(+)-ATPase (EC 3.6.

Modulation by cytosolic components of proton pump activities in plasma membrane and tonoplast from Cucumis sativus roots during salt stress.

The effect of NaCl on the plasma membrane and tonoplast ATPases measured as the hydrolytic and H(+)-pumping activity was studied. Treatment of cucumber seedlings with salt increased the membrane-bound ATPases of the plasma membrane as well as the tonoplast. In both types of membranes the stimulation of ATP-hydrolysis was much higher than the stimulation of H(+)-transport suggesting that the salt- treatment of plants partially uncoupled the membrane proton pumps.

Nitrate transport across the tonoplast of Cucumis sativus L. root cells.

Nitrate transport across the tonoplast has been studied using vacuole membranes isolated from cucumber roots grown in nitrate. The addition of NO3- ions into the tonoplast with ATP-generated transmembrane proton gradient caused the dissipation of delta pH, indicating the NO3(-)-induced proton efflux from vesicles. NO3(-)-dependent H+ efflux was almost insensitive to the transmembrane electrical potential difference, suggesting the presence of an electroneutral NO3-/H+ antiporter in the tonoplast.