[Isolation of shiga toxin-producing Escherichia coli strains from healthy cattle of the region of Lower Silesia].

The aim of the study was to determine if cattle from the region of Lower Silesia is the reservoir of shiga toxin-producing E. coli strains (STEC) and the analysis of virulence factors of isolated STEC strains. The ability of tested animal strains to shiga toxin synthesis was analysed in cytotoxicity assay in vitro on Vero cell line and then confirmed by detection of shiga toxin-encoding genes by PCR.