Assessment of proliferative activity of thyroid Hürthle cell tumors using PCNA, Ki-67 and AgNOR methods.

We have undertaken an attempt to compare the application efficacy of the proliferative activity markers in differential diagnosis of thyroid Hürthle cell tumors (HCT) using the PCNA and Ki-67 labeling and AgNOR visualisation techniques. The present work is a retrospective analysis of 78 Hürthle cell tumors: 20 Hürthle cell carcinomas (HCC), 32 Hürthle cell adenomas (HCA) and 26 hyperplastic nodules with Hurthle cell metaplasia (HCM). Five microm sections were stained according to AgNOR technique and labeled with antibodies against PCNA and Ki-67.

The influence of doxycycline on articular cartilage in experimental osteoarthrosis induced by iodoacetate.

The experiment was performed on 36 Wistar rats. On the first day of the experiment iodoacetate was administered to the left posterior knee joint of the 18 rats which composed Group I. The second group of 18 rats received additionally doxycycline (doxy) through the gastric tube in doses comparable with those of doxycycline used in humans.

AgNOR, Ki-67 and PCNA expression in fibroepithelial tumours of the breast in correlation with morphological features.

The authors retrospectively reviewed the cytological slides of 44 histopathologically confirmed fibroepithelial lesions of the breast, of which 11 were fibroadenoma (FA), 19 benign phyllodes tumours (PTLGM), 8 borderline (PTBM) and 6 malignant (PTHGM). The 2 FA misdiagnosed as PTLGM in cytological smears were both of cellular type. NORS were quantified in a series of the above cases using the silver-colloid method.

An evaluation of Ki-67 and PCNA expression in conjunctival and eyelid tumours.

The aim of our study was an evaluation of the expression of cell proliferation markers (PCNA and Ki-67) in conjunctival and eyelid papillomas and squamous and basal cell cancers. A series of 9 cases of squamous cell cancer (SCC), 15 cases of basal cell cancer (BCC) and 43 cases of squamous cell papilloma (SCP) were assessed using the immunohistochemical method with monoclonal antibodies. PCNA overexpression was observed in 100% of SCP, in 88.

Fetotoxic action of 2,4-dichlorophenoxyacetic acid (2,4-D). III. Morphological changes in rat kidneys.

The paper presents the findings of histological studies on the effect of sodium salt of 2,4-D acid on the changes within kidneys in newborn rats exposed to this herbicide in the prenatal and postnatal period. The experiment was performed on 60 Wistar rats of both sexes, up to 10 weeks of age. The animals were divided into two groups: I group (control)--18 rats fed on a standard diet and given tap water ad libitum, and group II (experimental)--42 rats, whose mothers received sodium salt of 2.