Background: Beneficial effects of mastication on cognitive abilities in the elderly have been shown in human studies. However, little is currently known about the effect of masticatory stimulation on cognitive and perceptual ability in younger populations. The purpose of the present study is to investigate the influences of masticatory stimulation on perceptual ability in adolescent boys.
View Article and Find Full Text PDFGonadotropin-inhibitory hormone (GnIH) is a newly identified hypothalamic neuropeptide that inhibits pituitary hormone secretion in vertebrates. GnIH has an LPXRFamide (X = L or Q) motif at the C-terminal in representative species of gnathostomes. On the other hand, neuropeptide FF (NPFF), a neuropeptide characterized as a pain-modulatory neuropeptide, in vertebrates has a PQRFamide motif similar to the C-terminal of GnIH, suggesting that GnIH and NPFF have diverged from a common ancestor.
View Article and Find Full Text PDFThe kiss1 peptide (kisspeptin), a product of the kiss1 gene, is one of the key neuropeptides regulating vertebrate reproduction. In 2009, we identified a paralogous gene of kiss1 in the brain of amphibians and named it kiss2. Currently, the presence of the kiss2 gene and the kiss2 peptide is still obscure in amniotes compared with that in other vertebrates.
View Article and Find Full Text PDFTwo cDNAs encoding gonadotropin receptors, follicle-stimulating hormone receptor (FSHR) and luteinizing hormone receptor (LHR) were cloned from mummichog (Fundulus heteroclitus) ovary. Deduced amino acid sequences of the mummichog FSHR (fhFSHR) and LHR (fhLHR) showed high homologies to teleost FSHRs (77-53%) and teleost LHRs (76-62%), respectively. Both the fhFSHR and fhLHR are composed of a typical structural architecture of glycoprotein hormone receptors consisting of the large N-terminal extracellular domain, the transmembrane domain containing seven cell surface membrane-spanning regions, and the intracellular domain.
View Article and Find Full Text PDFThe mummichog Fundulus heteroclitus is an excellent experimental fish for reproductive physiology because of its adequate size, easiness for rearing, and controllable reproduction under laboratory conditions. Furthermore, it is the only species that the native GtHs and their subunits have been purified among small experimental fishes. In this study, homologous non-competitive enzyme-linked immunosorbent assays (ELISAs) for the mummichog FSH and LH were developed by raising monoclonal and polyclonal antibodies against the purified GtHs or their subunits, and the plasma hormone levels in various seasons were examined.
View Article and Find Full Text PDFTo examine relationships between gonadal stages and the initial appearance and subsequent development of gonadotrophs, hatched larvae of the mummichog Fundulus heteroclitus were reared until first maturity under suitable conditions for maturation (20 degrees C-16L). Evident FSH cells generally appeared 1-2 weeks after hatching (wah), around or slightly before the morphological sex differentiation which occurred at 2 wah. During this period, 3beta-hydroxysteroid dehydrogenase positive cells also appeared in the gonads.
View Article and Find Full Text PDFComp Biochem Physiol B Biochem Mol Biol
June 2005
Myofibril-bound serine protease (MBSP) was purified from the myofibril fraction of white croaker (Argyrosomus argentatus) muscle and its enzymatic properties were compared with other fish MBSPs. White croaker MBSP was extracted by the heat treatment of myofibrils and then purified by a series of column chromatographies on Q-Sepharose, Sephacryl S-300, hydroxyapatite and Benzamidine Sepharose. The purified MBSP migrated as a single protein band at 67 kDa in SDS-PAGE under both reducing and non-reducing conditions.
View Article and Find Full Text PDFComp Biochem Physiol B Biochem Mol Biol
December 2004
A novel fish muscle serine protease named muscle soluble serine protease (MSSP) was purified from the soluble fraction of lizard fish (Saurida undosquamis: Synodontidae) muscle by ammonium sulfate fractionation followed by four steps of column chromatographies. In native-PAGE, the purified enzyme appeared as a single band with an estimated mol. mass of approximately 380 kDa by gel filtration.
View Article and Find Full Text PDFComp Biochem Physiol B Biochem Mol Biol
January 2004
Myofibril-bound serine protease (MBSP) from lizard fish (SAURIDA UNDOSQUAMIS: Synodontidae) skeletal muscle was purified to homogeneity with higher purification (1260-fold) and higher recovery (7%) than our previous report in lizard fish (Saurida wanieso). The new purification method combines a heat-treatment for dissociation from washed myofibrils, acid-treatment at pH 5.0 before and after lyophilization, and alcohol-treatment, followed by two column chromatographies.
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