A quasi-randomized controlled trial of Ninjin'yoeito for the treatment of postpartum anemia and the prevention of postpartum depression.

Aim: To study the effect of Ninjin'yoeito (NYT) on postpartum anemia and on the development of postpartum depression (PPD).

Methods: In this prospective, single-center, open-label, quasi-randomized controlled trial, patients with anemia 1-2 days postdelivery were randomized to receive either NYT or an oral iron preparation for 4 weeks. The primary endpoint was the hemoglobin (Hb) level.

Subretinal implantation of Okayama University-type retinal prosthesis (OUReP) in canine eyes by vitrectomy.

Okayama University-type retinal prosthesis (OUReP) is a photoelectric dye-coupled polyethylene film which generates electric potential in response to light and stimulates nearby neurons. This study aims to test surgical feasibility of subretinal implantation and functional durability of dye-coupled films in the subretinal space. The dye-coupled films were implanted subretinally by 25-gauge vitrectomy in the right eye of 11 normal beagle dogs: 2 dogs served for film removal after 5-month film implantation, 3 dogs for film removal after 3-month film implantation, 3 dogs for 3-month film implantation and pathological examination, and 3 dogs for sham surgery.

Structure-activity relationship study on alpha1 adrenergic receptor antagonists from beer.

Hordatine A and aperidine have been previously isolated from beer as active ingredients, which bind to muscarinic M3 receptor. In addition, these compounds have exhibited antagonist activity against the alpha1A adrenoceptor. Although the relative structures of these two molecules have previously been determined, the absolute stereochemistry was unclear.

Structural changes and cell properties of human ovarian surface epithelium in ovarian pathophysiology.

The surface epithelial cells of the ovary, which are modified peritoneal cells, form a single, focally pseudostratified layer. The Müllerian ducts differentiate after invagination of the coelomic mesothelium over the gonadal ridges during the 6th week of embryonic life. On the basis of the embryologically putative Müllerian potential of this epithelium, endometriosis can be explained by coelomic metaplasia from the peritoneum, including ovarian surface epithelium.

Synthesis, properties, and NAD+-NADH-type redox ability of 14-substituted 1,3-dimethyl-5,10-methanocycloundeca[4,5]pyrrolo[2,3-d]pyrimidine-2,4(1,3h)-dionylium tetrafluoroborates and their hydride adducts.

A synthesis of 14-substituted 1,3-dimethyl-5,10-methanocycloundeca[4,5]pyrrolo[2,3-d]pyrimidine-2,4(1,3H)-dionylium tetrafluoroborates 11a,b(+).BF4- was accomplished by the methylation of 5,10-methanocycloundeca[4,5]pyrrolo[2,3-d]pyrimidine-2,4(1,3H)-dione derivatives with MeI and following anion-exchange reaction by treatment with 42% aq HBF(4). Compound 11b(+).

Synthesis, properties, and oxidizing ability of areno-annulated 1,3-dimethyl-10-phenylcyclohepta[4,5]pyrrolo[2,3-d]pyrimidine- 2,4(1,3H)-dionylium ions.

[structures: see text] Novel areno-annulated 1,3-dimethyl-10-phenylcyclohepta[4,5]pyrrolo[2,3-d]pyrimidine-2,4(1,3H)-dionylium ions 12a,b+ x BF4(-) and 16a+ x BF4(-) were synthesized by three-step reactions, starting from the reactions of benzo[b]tropone and naphtho[2,3-d]tropone with 6-anilino-1,3-dimethyluracil. Structural characteristics of 12a,b+ and 16a+ were clarified on inspection of the UV-vis and NMR spectral data as well as by X-ray crystal analyses. The stability of cations 12a,b+ and 16a+ is expressed by the pK(R+) values which were determined spectrophotometrically as the values of ca.

Novel synthesis, properties, and NAD+-NADH type redox ability of 1,3-dimethylcyclohepta[4,5]pyrrolo[2,3-d]pyrimidine- 2,4(1,3h)-dionylium ions annulated with additional pyrrolo[2,3-d]pyrimidine-1,3(2,4h)-dione and furan analogue, and their hydride adducts.

[reaction: see text] A convenient preparation of novel cations 11a,b+ x BF4(-) and 12a,b+ x BF4(-), which are derived from annulation of the 1,3-dimethylcyclohepta[4,5]pyrrolo[2,3-d]pyrimidine-2,4(1,3H)-dionylium ion with additional pyrrolo[2,3-d]pyrimidine-1,3(2,4H)-dione and a furan analogue, was accomplished by a novel oxidative cyclization of 1,7-dihydro-7-[1',3'-dimethyl-2',4'(1',3'H)-dioxo-6'-(phenylamino)-pyrimidin-5'-yl]-1,3-dimethyl-10-phenylcyclohepta[4,5]pyrrolo[2,3-d]pyrimidine-2,4(1,3H)-dione 9 and its furan-analogue by using DDQ or photoirradiation under aerobic conditions. Structural characteristics of 11a,b+ and 12a,b+ were clarified on inspection of the UV-vis and NMR spectral data as well as X-ray crystal analyses. The stability of cations 11a,b+ and 12a,b+ is expressed by the pK(R+) values that were determined spectrophotometrically to be 10.

Novel synthesis, static and dynamic properties, and structural characteristics of 5-cyano[n](2,4)pyridinophane-6-ones (n= 9-6) and their chemical transformations.

A novel synthesis of 5-cyano[n](2,4)pyridinophane-6-ones 12a-d (n= 9, 8, 7, and 6) consists of allowing cyanoacetatoamide to react with cycloalk-2-enones. Their static and dynamic properties as well as structural characteristics are studied on the basis of their spectroscopic properties, cyclic voltammetry, and theoretical calculations. The (1)H and (13)C NMR spectra at various temperatures have clarified the dynamic behavior of the methylene chains for [7](2,4)- and [6](2,4)pyridinophane-6-one derivatives 12c and 12d.

New synthesis, properties, and oxidizing ability of 1,3-dimethyl-5,10-methanocycloundeca[4,5]furo[2,3-d]pyrimidin- 2,4(1,3H)-dionylium tetrafluoroborate.

A novel synthesis of 1,3-dimethyl-5,10-methanocycloundeca[4,5]furo[2,3-d]pyrimidin-2,4(1,3H)-dionylium tetrafluoroborate (10(+).BF(4)(-)) was accomplished by the reaction of 3,8-methano[11]annulenone with dimethylbarbituric acid and following acidic cyclization, albeit in low yield. Remarkable structural characteristics were suggested on inspection of the spectral data and MO calculation, and it was clarified that the positive charge is largely localized at the C11.

Inhibitory effect of (-)-epigallocatechin 3-gallate, a polyphenol of green tea, on neutrophil chemotaxis in vitro and in vivo.

The effect of (-)-epigallocatechin 3-gallate (EGCG), a major polyphenol of green tea, on neutrophil migration has been studied using multiwell-type Boyden chambers in vitro and a fluorescein isothiocyanate-labeled ovalbumin (FITC-OVA)-induced rat allergic inflammation model in vivo. EGCG inhibited rat neutrophil chemotaxis toward cytokine-induced neutrophil chemoattractant-1 (CINC-1) in a concentration-dependent manner. In addition, CINC-1-induced neutrophil chemotaxis was suppressed by the pretreatment of rat neutrophils with EGCG at the concentration over 15 microg/mL.

Novel synthesis and oxidizing ability of tropylium ions annulated with two 2,4-dimethylfuro[2,3-d]pyrimidine-1(2H),3(4H)-diones.

Convenient preparation of novel tropylium ions annulated with two 2,4-dimethylfuro[2,3-d]pyrimidine-1(2H),3(4H)-diones, 12a(+).BF(4)(-) and 12b(+)().BF(4)(-), consists of a reaction of 2-methoxytropone with dimethylbarbituric acid to give 7,9-dimethyl-3-[1',3'-dimethyl-2'(1'H),4'(3'H),6'(5'H)-trioxopyrimidin-5'-ylidene]cyclohepta[b]pyrimido[5,4-d]furan-8(7H),10(9H)-dione 8 and the following oxidative cyclization by using DDQ or photoirradiation under aerobic conditions.

Decreased expression of 14-3-3 sigma is associated with advanced disease in human epithelial ovarian cancer: its correlation with aberrant DNA methylation.

Purpose: In this study, we examined the promoter methylation status and expression of 14-3-3 sigma and evaluated its clinical significance in epithelial ovarian cancer.

Experimental Design: Twelve ovarian cancer cell lines; 2 ovarian surface epithelial cell lines; and 8 normal, 8 benign, 12 borderline, and 102 ovarian cancer tissues were examined. Methylation-specific PCR, quantitative reverse transcription-PCR, and immunohistochemistry were used to evaluate methylation status and expression of 14-3-3 sigma gene and protein.

Novel synthesis and properties of 1,6-methanocycloundeca[b]pyrimido[5,4-d]pyrrole-12,14-dione derivatives: autorecycling oxidation of some amines and alcohols.

Novel 1,6-methanocycloundeca[b]pyrimido[5,4-d]pyrrole-12,14-dione derivatives 6a,b and 7a-c were synthesized in moderate to good yields by the enamine alkylation and dehydrating condensation reactions of 11-chloro-3,8-methano[11]annulenone (8) with 6-amino-3-substituted uracil derivatives and subsequent elimination of HCl. The (1)H NMR spectra clarified that compounds 6a,b and 7a-c are aromatic molecules having a diatropic pi-system, which is suggested by the chemical shifts of the bridge methylene protons and peripheral protons. The electrochemical reduction exhibited more positive reduction potentials as compared with those of the vinylogous compounds of cyclohepta[b]pyrimido[5,4-d]pyrrole derivatives.

Synthesis, properties, and oxidizing ability of 9,11-dimethylbenzocyclohepta[6,7-b]pyrimido[5,4-d]furan- 10(9H),12(11H)-dionylium tetrafluoroborate.

Convenient preparation of novel 9,11-dimethylbenzocyclohepta[6,7-b]pyrimido[5,4-d]furan-10(9H),12(11H)-dionylium tetrafluoroborate 11*BF4- consisted of a condensation reaction of benzo[d]tropone with dimethylbarbituric acid and following oxidative cyclization reaction by using DDQ-Sc(OTf)3 or photoirradiation under aerobic conditions. Alternative synthesis of 11*BF4- was also accomplished by a condensation reaction of 2-methoxybenzo[d]tropone with dimethylbarbituric acid and a subsequent cyclization reaction by using 42% aq HBF4. The X-ray crystal analysis and MO calculation were carried out to clarify the structural characteristics.

Novel synthesis, properties, and oxidizing ability of 11,13-disubstituted 3,8-methanocycloundeca[8,9-b]pyrimido[5,4-d]-furan-12(11H),14(13H)-dionylium tetrafluoroborates.

Synthesis of novel 4,9-methanoundecafulvene [5-(4,9-methanocycloundeca-2',4',6',8',10'-pentaenylidene)pyrimidine-2(1H),4(3H),6(5H)-trione] derivatives 10a-c was accomplished. Their structural characteristics were investigated on the basis of the 1H and 13C NMR and UV-vis spectra. Upon treatment with DDQ, 10a-c underwent oxidative cyclization to give novel 11,13-disubstituted 3,8-methanocycloundeca[8,9-b]pyrimido[5,4-d]furan-12(11H),14(13H)-dionylium tetrafluoroborates 11a-c*BF4- in good yields.

Roles of luteinizing hormone/chorionic gonadotropin receptor in anchorage-dependent and -independent growth in human ovarian surface epithelial cell lines.

Epithelial ovarian carcinomas are thought to arise from cells of ovarian surface epithelium (OSE) covering the free surface of the human ovary. Two immortalized human cell lines, OSE2a (non-tumorigenic) and OSE2b-2 (tumorigenic), were previously established from normal OSE cells of a reproductive-age patient. In the present study, we found that expression of luteinizing hormone (LH)/chorionic gonadotropin (CG) receptor (LH/CGR) is present in OSE2a cells and absent in OSE2b-2 cells.

Synthesis, stability, and spectroscopic and electronic properties of three benzocyclohepta[alpha]azulenylium ions.

Novel pi-systems, three benzocyclohepta[alpha]azulenylium ions, 7a-c, are synthesized, and their stability and properties have been characterized in terms of the position of the benzo-annulation and compared with those of the parent cyclohepta[alpha]azulenylium ion 4. Benzocyclohepta[6,7-alpha]azulenylium ion (7a) (p K R+ = 7.3, E red = -0.