Publications by authors named "Makiichi Takagaki"
Article Synopsis
- * A specific genetic mutation (V75M) was identified in the resistant strain, which involves a change from valine to methionine at position 75 of the SDH protein.
- * Cloning experiments showed that the engineered SDH proteins from both strains had the same resistance profiles as their respective natural SDH, confirming that the V75M mutation is responsible for the reduced sensitivity to carpropamid in the resistant strain.
Rice blast fungus isolates were collected in Kyushu to investigate resistance to scytalone dehydratase inhibitors of melanin biosynthesis (MBI-D). In 2001, failure of control of rice blast was reported in the Saga prefecture, where MBI-Ds have been used since 1998. At that time, the distribution of resistant isolates was mainly limited to that area.
View Article and Find Full Text PDFWe have established a simple diagnosis method for rice blast fungus resistant to MBI-D. This involves the preparation of PCR templates directly from the lesions in combination with primer-introduced restriction enzyme analysis PCR (PIRA-PCR).
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