Publications by authors named "Maki Kanda"

Veterinary drugs are used worldwide to prevent and treat diseases and promote growth in animals, fisheries, and beekeeping. Despite their effectiveness, the illegal and improper use of these drugs can result in livestock and fishery products, potentially impacting human health by causing allergic reactions, cytotoxicity, and antimicrobial resistance. To mitigate these adverse effects, the Japanese government established a positive list system in 2006.

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  • The study introduced a new microbiological method using a microtiter plate to detect 10 types of sulfonamide drugs in chicken muscles, eggs, and prawns, proving to be eco-friendly compared to traditional methods.
  • The method involved a bioassay for initial residue detection, followed by confirmation and quantification using LC-MS/MS, achieving detection limits that meet Japan's minimum residual limits (MRLs).
  • Quality control tests showed effective performance of the bioassay, and a survey found sulfamethoxazole in one prawn sample out of 91 tested, demonstrating the method's practical application.
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  • The study focuses on developing a screening method to detect cereulide, a toxin from Bacillus cereus, in fried rice, which is important for identifying food poisoning cases even when the bacteria itself may not be found.
  • The researchers used the QuEChERS extraction method along with LC-MS/MS to identify and quantify cereulide, achieving accuracy levels between 88% and 89%, and establishing a limit of quantification at 2 µg/kg.
  • Results indicated that the method effectively removed interfering compounds and provided reliable quantification, meeting Japanese validation guidelines, thus showing potential as a dependable tool for checking fried rice for cereulide contamination.
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  • A new method using LC-MS/MS was developed to simultaneously detect five types of carbapenem antibiotics (biapenem, doripenem, ertapenem, imipenem, meropenem) in both raw and pasteurized bovine milk samples.* -
  • The method involved chromatographic separation on a specialized column, extraction processes to remove lipids and proteins, and validation according to Japanese guidelines, showing good accuracy and reproducibility.* -
  • Testing of 60 milk samples from Tokyo found no traces of the carbapenems, indicating the method's effectiveness for routine monitoring of these antibiotics in milk.*
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  • A rapid analytical method was validated for detecting 20 different veterinary drugs in poultry muscle, eggs, and raw milk using LC-MS/MS technology.
  • The method involves extracting analytes with acetonitrile and then purifying them with magnesium sulfate, trisodium citrate, and sodium chloride.
  • In tests, 17 out of 20 drugs were detectable in chicken and duck muscle, while all drugs were found in eggs and raw milk, suggesting further monitoring for veterinary drugs is needed as some residues were detected below the quantification limits.
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  • This study evaluated the Charm QUAD2® Test, an immunochromatographic method, for detecting residual macrolides and lincosamides in raw cow's milk, showing high selectivity and a 0% false-positive rate.
  • The test met validation parameters according to European Commission standards, demonstrating low detection limits for various antibiotics that are below most maximum residue limits in Japan, except for lincomycin.
  • Results from the test correlated well with traditional chemical and microbiological methods, indicating its potential for routine food safety analysis in milk.
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  • The study aimed to develop a new technique for detecting Clostridium perfringens enterotoxin (CPE) in human stool, which is important for diagnosing food poisoning.
  • It involved spiking CPE into artificial gut fluid and using stable isotope-labelled peptides alongside liquid chromatography-tandem mass spectrometry (LC-MS/MS) for analysis.
  • The developed method successfully quantified CPE with good recovery rates and low standard deviations, making it a reliable tool for future assessments of CPE.
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  • Researchers used a specialized LC-MS/MS method to analyze 127 honey samples from Tokyo for residual acaricides between April 2015 and March 2021.
  • Amitraz was found in 85 samples at levels ranging from 1.1 to 34.1 μg/kg, while propargite was detected in 3 samples at 2.4 to 3.8 μg/kg, but all results were within Japanese maximum residue limits (MRLs).
  • The consistent presence of amitraz over the years indicates proper usage in beekeeping, while the detection of propargite for the first time in 2020 may point to a changing trend in acaricide applications in Japan.
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Residual antibacterial agents in 5909 animal and fishery products in Tokyo, Japan, were investigated over 17 consecutive years (2003-2019). Monitoring of 32 antibacterial agents (lincosamides, macrolides, penicillins, quinorones and tetracyclines) per product was accomplished via two steps: screening (by microbiological methods) and confirmation (by instrumental methods). Microbiological screening methods identified presumptive groups and determined semi-quantitative values.

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To recognize the risk of Bacillus cereus in pasteurized milk, we investigated the prevalence of B. cereus and the rate of the production of cereulide from B. cereus isolates.

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Salt intake reduction is crucial to prevent non-communicable diseases (NCDs) globally. This study aimed to investigate the short- and long-term effects of monitoring salt concentration in homemade dishes on reducing salt intake in a Japanese population. A double-blind randomized controlled trial using a 2 × 2 factorial design with two interventions was conducted in 195 participants; they were assigned to both interventions for a group monitoring salt concentration in soups (control: no monitoring) and a group using low-sodium seasoning (control: regular seasoning).

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The determination of antibacterial agents for animals in swine muscles was improved by microbiological screening and liquid chromatography-mass spectrometry (LC-MS/MS) analyses. In the first instance, the residual drugs were extracted from the samples using the NaEDTA-McIlvaine buffer (pH 6.0).

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  • Researchers created a specific labelled protein to detect non-toxic peptides from botulinum neurotoxin type A (BoNTA) in honey using advanced techniques like liquid chromatography-tandem mass spectrometry (LC-MS/MS).
  • They employed a careful two-step process to isolate proteins from honey, ensuring effective digestion and removing interference from salts.
  • The method successfully identified unique non-toxic peptides, confirming no BoNTA presence in 19 honey samples tested, highlighting its potential for improving food safety monitoring.
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We developed an analytical method for determining 15 antifungal drugs, 2 antiparasitic drugs, and 3 veterinary drugs in fish and livestock products using LC-MS/MS. First, 50% ethanol was added to their products, and the mixture was homogenized to reduce drug degradation. Thereafter, 20 drugs were extracted from the pretreated sample mixture using acetonitrile.

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In this study, the staphylococcal enterotoxin type A (SEA) contaminant was quantified in cow milk by liquid chromatography-tandem mass spectrometry (LC-MS/MS) with the use of a stable isotope-labelled peptide of SEA as an internal standard. SEA was cleaned up in a two-step process that included pH control and trichloroacetic acid (TCA) precipitation. The pH control phase eliminated other proteins.

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In this study, the presence of cereulide in cow's milk was identified and quantified using our validated method with liquid chromatography-tandem mass spectrometry. Cereulide was concentrated using protein acid-precipitation and extracted from the precipitate by using acetonitrile twice. The combination of protein acid-precipitation and extraction sufficiently eliminated the matrix compounds from the milk and a further clean-up step utilising solid-phase extraction could be omitted.

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  • A validated LC-MS/MS method was used to determine cyromazine levels in livestock products, including milk and eggs.
  • The extraction process optimized methanol and pH 3.0 buffer, followed by cleanup using a special cartridge and chromatography.
  • The method met Japanese guidelines, showing recovery rates of 77.2 to 92.1%, with excellent precision indicated by low relative standard deviations (RSDr).
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We developed a method for the simultaneous determination of acaricides in comb honey using LC/MS/MS. Because methods for honey analysis had not previously been applied to comb honey, we modified three techniques for sample preparation and LC/MS/MS conditions. First, we used a modified QuEChERS method that changed the extraction solution from ethyl acetate to acetonitrile.

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We developed a simultaneous determination method for 37 veterinary drugs in two chicken processed foods (deep-fried chicken and non-fried chicken cutlet) and muscle via liquid chromatography-mass spectrometry. The veterinary drugs belong to 7 different classes, including 4 antifolics, 4 benzimidazoles, 5 macrolides, 7 polyethers, 2 quinolones, 7 sulfonamides, and 8 other classes. The samples were extracted with ethyl acetate followed by acetonitrile with salt and buffers extraction.

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A simultaneous determination of amantadine, rimantadine, and memantine in processed products (deep-fried chicken, fried chicken, fried quail egg, and grilled chicken) with liquid chromatography tandem mass spectrometry (LC-MS/MS) was developed. This new method was also applicable for chicken tissue (muscle, liver, and gizzard) and eggs. The chromatographic separation was performed on a Kinetex XB-C18 core-shell technology column using a mobile phase of acetonitrile and 0.

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A simple analytical method for the determination of hydrocortisone and progesterone in bovine, swine, and chicken muscle and eggs was developed. Hydrocortisone and progesterone were extracted with acetonitrile and subsequently cleaned-up using an Oasis HLB mini-cartridge. The method was validated in accordance with Japanese guidelines and exhibited trueness from 86.

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An accurate and selective analytical method for amantadine, which is used as antiviral drug to treat influenza A virus infection, was developed using LC-MS/MS. Residual amantadine was extracted from 4 kinds of food sample (poultry muscle, liver, gizzard and egg) with acetonitrile-pH 3.0 McIlvaine buffer (7 : 3), then cleaned up with an Oasis® MCX mini-cartridge.

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A simple and accurate analytical method for the determination of acaricides in honey was developed and validated in accordance with Japanese validation guidelines. Analytes - amitraz, N-2,4-dimethylphenyl-N-methylformamidine (DMPF), etoxazole, fenpyroximate, fipronil, hexythiazox, propargite, pyridaben and spirodiclofen - were extracted with ethyl acetate under basic conditions and subsequently cleaned up using an InertSep(®) MA-1 polymer-based anion-exchange column. The method was validated by fortified recovery tests at three different concentrations (1, 5 and 10 µg kg(-1)) performed with three samples daily on five different days.

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Residues of 37 polar veterinary drugs belonging to six families (quinolones, tetracyclines, macrolides, lincosamides, sulfonamides, and others) in livestock and fishery products were determined using a validated LC-MS/MS method. There were two key points in sample preparation. First, extraction was performed with two solutions of different polarity.

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A method of rapid analysis of multi-class residual veterinary drugs in milk, fish and shellfish was validated in accordance with Japanese guidelines for the validation of analytical methods for residual agricultural chemicals in food. Using LC-MS/MS, 43 multi-class veterinary drugs, including sulfonamides, quinolones, coccidiostats and antiparasites, could be analyzed in one injection. Analytes were extracted from samples with two kinds of solvent, acetonitrile containing 1 vol% formic acid and anhydrous acetonitrile, and salted out with 4.

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