[Comparison of biological activity of high molecular weight and low molecular weight forms of immunoreactive prolactin from human serum in cultured lymphoma NB2 cells].

In experiments with NB2 rat lymphoma cells culture sensitive to lactogenic hormones the mitogenic activity of high molecular weight (> OOK) immunoreactive prolactin, found in substantial quantities in serum of certain hyperprolactinemic women, as compared to the activity of serum low molecular weight (23K) form, was studied. It was established that the ratio of immunoreactive to biologically active prolactin content in serums in cases of low molecular weight form predominance is close to 1,0 whereas in case of predominant content of high molecular weight form it is substantially higher (1.5-2.

[Heterogeneity of immunoreactive prolactin in hyperprolactinemia of various origin].

Study of molecular heterogeneity of immunoreactive prolactin in patients with macro- and microprolactinomas and idiopathic hyperprolactinemia has shown heterogeneity of the total blood immunoreactive prolactin pool in all the examined patients. This pool included three basic forms with molecular masses about 23, 50, and over 100 kD (23K-, 50K-, 100K-prolactin), whose ratios essentially differed in individual patients. Physiologically the most active monomeric 23K form of prolactin predominated in the blood of patients with hyperprolactinemia due to hypophyseal micro- and macroadenomas, parallelled by manifest signs of galactorrhea and hypogonadism; the content of this form may reach 95% of the total immunoreactive hormone.

[Physico-chemical, immunochemical, and biological activity of prolactin from human amniotic fluid].

Highly purified preparation of prolactin with molecular mass 23 kDa, which is the main form of the hormone in human amnionic fluid, was isolated from the fluid using gel filtration on Sephadex G-100, chromatography on DEAE-Sepharose, concanavalin A Sepharose, CM-cellulose. Physico-chemical, immunochemical and biological properties of the amnionic prolactin were studied as compared with those of hypophyseal hormone. Properties of the hormonal forms isolated from both these tissues were similar.

[Polymorphism of prolactin in amniotic fluid of women at various times of pregnancy].

The level and molecular forms of prolactin (PRL) in amniotic fluid of pregnant women were investigated at various time of pregnancy with the help of radioimmunoassay, electrophoresis in polyacrylamide gel and immune blotting technique using poly- and monoclonal antibodies to human hypophyseal prolactin (PRL). Changes in the level of immunoreactive PRL in amniotic fluid had the shape of a Gaussian curve with a maximum at 22-29 weeks. Amniotic PRL was characterized by molecular polymorphism.

[The role of periodic changes in cyclase and protein kinase activity in the mechanism of the proliferative effect of ACTH].

A prolonged effect of ACTH on the state of adenylate and guanylate cyclase systems in the adrenal glands of experimental animals was investigated. It was found that in guinea pigs injected with ACTH (4 units daily for 1-50 days) the weight of adrenal glands and the DNA content in these organs increased 2.0-2.

[Species and organ differences in the activity and regulation of adenylate and guanylate cyclases].

The activity of adenylate and guanylate cyclases was determined in adrenal, heart, liver and fat tissues of guinea pigs, mice, rabbits and monkeys. The enzymes activities varied markedly depending both on the species and organs. The highest basal activities of adenylate cyclase was observed in all organs of guinea pigs.

[Adenylate- and guanylate cyclase systems in pathology of the adrenal glands].

The authors have summed up the results of a study of the activity of adenylate and guanylate cyclase system in the adrenals of patients with the Icenko-Cushing disease and syndrome and in the adrenals of experimental animals after unilateral adrenalectomy and in ACTH administration. It has been established that the biochemical mechanisms of compensatory hypertrophy of the adrenals in the normal and pathological tissue differ significantly. It has been shown for the first time that an increase in the basal activity of adenylate cyclase, resulting in the restoration of the initial hormonal level, is an obligatory condition for the compensation of organ function.

[Adenylate cyclase and cAMP-dependent protein kinase activity in the adrenal glands of animals after chronic administration of ACTH].

ACTH, a prolonged action hormone, in a dose of 2.5 mu. was injected into guinea pigs daily for 5-35 days.

[Inhibitory effect of 18S ribosomal RNA of different origin on a wheat embryo cell-free translation system].

The action of 18S rRNAs from three different sources--mouse reticulocytes, Ehrlich ascite carcinoma cells and wheat embryo--on cell-free translational system derived from wheat embryo was investigated. It is shown that all rRNA preparations have similar inhibitory action on translational activity of the system. This action is not dependent on the source of rRNA and mRNA by which the system is primed.

[Isolation and characteristics of partially purified procollagen mRNA from human fibroblasts].

Procollagen 27S mRNA was isolated from heavy polysomes of human embryo fibroblasts. This RNA directed the synthesis of collagenase sensitive polypeptides in wheat germ translation system and was active in reverse transcription.

[Inhibitory effect of 18S-rRNA from mouse reticulocytes on translation of exogenous and endogenous mRNA in a cell-free system of wheat embryos].

The effect of 18S-rRNA from mouse reticulocytes on the translation of exogenous and endogenous matrices in a system of wheat embryos was studied. It was found that after addition of 18S-rRNA the translation of exogenous and endogenous mRNAs is strongly inhibited. Treatment of 18S-rRNA with proteinase K prior to the addition did not remove the inhibiting action of rRNA.

[Application of nitrocellulose Nitrocell S for preparation of mouse globin mRNA].

Two methods of chromatography were applied and compared to purify individual mRNA for mouse globin. In one case commercial product Nitrocell S was used, in another affinity chromatography on oligo-dT-cellulose was applied. Translation of purified mRNA was performed in the heterological wheat germ system in vitro.

[Evaluation of the degree of mitral valve stenosis by the data of polycardiography and dye dilution technic].

An equation for calculating the mean pressure in the left atrium is suggested. The area of the mitral orifice was calculated according to the Gorlin's equation on the basis of the data of polycardiography and the dye dilution method. The mean pressure in the left atrium was determined in 129 patients with acquired heart failures, the area of the mitral orifice--in 36 patients.