NEUTROPHIL TRAPS AS AN IMMUNE RESPONSE MECANISM IN PETIENTS WITH EROSIVE DISEASES OF THE UPPER GASTROINTESTINAL TRACT.

To optimize the methods for determining neutrophil extracellular traps in peripheral blood and studying their level in the norm and in patients with candidiasis with inflammatory and erosive diseases of the upper gastrointestinal tract on the basis of the Institute of Gastroenterology of the National Academy of Medical Sciences and Clinical Association of Emergency Medicine, Dnipro, Ukraine, in 2019-2021. 42 patients with candidiasis with inflammatory and erosive diseases of the upper gastrointestinal tract were examined, including 10 women and 32 men aged 35-56 years, who made up the main group (MG). These patients were divided into two groups according to the results of microbiological examination of the mucous membranes of the gastrointestinal tract: group 1 consisted of 20 patients with oropharyngeal candidiasis of the oral cavity; Group 2 consisted of 22 patients with candidiasis in the esophagus and stomach with or without oropharyngeal candidiasis.

Proteomic Markers in the Muscles and Brain of Pigs Recovered from Hemorrhagic Stroke.

(1) Background: Stroke is the leading cause of serious long-term disability. Walking dysfunction and paresis of the upper extremities occurs in more than 80% of people who have had a stroke. (2) Methods: We studied post-genomic markers in biosamples of muscle and brain tissue from animals that underwent intracerebral hematoma and recovered after 42 days.

PREOPERATIVE PREPARATION OF DONOR ZONES OF PERFORANT FLAPS BY THE METHOD OF TRAINING PERFORANT VESSELS WITH NEGATIVE PRESSURE.

One of the key factors in achieving favorable treatment outcomes when planning reconstructive surgeries using perforant flaps is the choice of recipient vessels. Their identification causes certain difficulties both at the planning stage and during the operation. The aim of the study was to develop the most rational modes of action of negative pressure on the skin of the thigh.

RESEARCH OF THE TEACHING EFFICIENCY THE SECTION "THERMAL TRAUMA" USING INTERACTIVE TECHNOLOGIES.

The goal of research is the formation of educational community of students who have been certain skills and ready to acquire new knowledge in the process of communicating with each other, joint cognitive activity through the use of interactive technologies - the method of cooperative groups. This method is used to search for common features in different schemes of medical care for patients with burns. The study involved 8 student groups of the 3rd year of the Faculty of Medicine, State Medical University, Dnipro.

Local Experimental Intracerebral Hemorrhage in Rats.

(1) Background: Hemorrhagic stroke is a lethal disease, accounting for 15% of all stroke cases. However, there are very few models of stroke with a hemorrhagic etiology. Research work is devoted to studying the development of cerebrovascular disorders in rats with an intracerebral hematoma model.

SCIATIC NERVE REGENERATION AFTER AUTOGRAFTING AND APPLICATION OF THE BONE MARROW ASPIRATE CONCENTRATION.

The work aims at studying the effect of the autologous bone marrow aspirate concentrate on regeneration of the sciatic nerve and atrophy of m. tibialis cranialis. We have simulated autografting of the sciatic nerve in rabbits with application of bone marrow aspirate concentrate around the graft area.

Muller's Ratchet and Ribosome Degeneration in the Obligate Intracellular Parasites .

are fungi-like parasites that have the smallest known eukaryotic genome, and for that reason they are used as a model to study the phenomenon of genome decay in parasitic forms of life. Similar to other intracellular parasites that reproduce asexually in an environment with alleviated natural selection, experience continuous genome decay that is driven by Muller's ratchet-an evolutionary process of irreversible accumulation of deleterious mutations that lead to gene loss and the miniaturization of cellular components. Particularly, have remarkably small ribosomes in which the rRNA is reduced to the minimal enzymatic core.

Error-prone protein synthesis in parasites with the smallest eukaryotic genome.

Microsporidia are parasitic fungi-like organisms that invade the interior of living cells and cause chronic disorders in a broad range of animals, including humans. These pathogens have the tiniest known genomes among eukaryotic species, for which they serve as a model for exploring the phenomenon of genome reduction in obligate intracellular parasites. Here we report a case study to show an apparent effect of overall genome reduction on the primary structure and activity of aminoacyl-tRNA synthetases, indispensable cellular proteins required for protein synthesis.

Ambiguity of mapping the relative phase of blood pulsations.

Blood pulsation imaging (BPI) is a non-invasive optical method based on photoplethysmography (PPG). It is used for the visualization of changes in the spatial distribution of blood in the microvascular bed. BPI specifically allows measurements of the relative phase of blood pulsations and using it we detected a novel type of PPG fast waveforms, which were observable in limited areas with asynchronous regional blood supply.

[Simulation of repeated local hemorrhagic stroke in rats].

The processes of developed in CNS the complicated stroke and developments of fittings for their pharmaceutical therapy were developed and offering by standardized method of the experimental secondary stroke in rats, suitable for the use in sharp and chronic researches. Variant of repeated hemorrhagic stroke consist of autohemorrhagic right hemisphere stroke by the mechanical damage of brain tissue after 10-daily occlusion of right common carotid artery was studied. A model is comfortable for reproducing of the repeated standardized local damage of brain, is more adequate form of design of transient and chronic cerebrovascular pathology, than the independent use of local hemorrhage of autoblood in the brain of animals.

Atrioventricular nodal reentrant tachycardia treatment using novel potential.

Radiofrequency ablation of atrioventricular nodal reentrant tachycardia is commonly guided by slow and sharp bipolar potentials of the atrioventricular slow nodal pathway. We optimized the morphology of the guiding potential by unipolar mapping of the slow nodal pathway. We identified a novel unipolar dual-component atrial electrogram at the anterior limb of the coronary sinus ostium.

[Procedures for modeling of hemorrhagic stroke].

Hemorrhagic stroke (intracerebral hemorrhage) is a socially significant cerebrovascular disease. Despite high mortality and disability rates, hemorrhagic stroke has been experimentally studied to a lesser extent than ischemic stroke. The modeling of hemorrhagic stroke may be used to solve a number of fundamental and practical problems.

[Influence of cortical neurotrophic factors on the neurocytokine production system in acute hemorrhagic stroke].

The mechanism of therapeutic action of cortical neurotropic factors (CNTF) was studied in hemorrhagic stroke. In intracerebral hemorrhage, CNTFs were shown to elevate the level of nerve growth factor mRNA and at the same time, produce no effect on its level in intact animals. The neuroactivating action of CNTF in the acute phase of hemorrhagic stroke was achieved by intranasal administration due to the retrograde axon transport of CNTF molecules along the olfactory nerve fibers to the brain, by passing the blood-brain barrier.

[Cytoprotective effect of neuropeptides on immunocompetent cells (in vitro study)].

Effects of the nootropic neuropeptide drugs cerebrolysin (Ebewe, Austria) and cortexin (Geterofarm, Russia) on the immunocompetent cells (T-lymphocytes of the MT-4 cell line and B-lymphocytes of the Raji cell line) were studied in vitro. The cell viability was evaluated using the MTT test by counting living and dead cells upon incubation under various conditions with a vital stain (Trypan Blue). It is established that cerebrolysin exhibits cytoprotective properties with respect to both T- and B-lymphocytes and favors the survival of immunocompetent cells.

[The role of latent virus infection of the central nervous system in acute hemorrhagic stroke].

The purpose of the investigation was to study whether latent virus infection may activate in the murine brain using a model of hemorrhagic stroke. Acute intracerebral hemorrhagic stroke was induced in the internal capsule in accordance with the original technology. For experimental reproduction of virus meningoencephalitis, albino mice were infected with a sublethal dose of herpes simplex virus.

[Dynamics of neurodegenerative processes and lifespan under the action of Cerebral combined with other pharmaceuticals in Drosophila melanogaster mutants].

Effective influence of the drug Cerebral and its micro- and macrofractions on the mean lifespan and degenerative process dynamics of Drosophila melanogaster have been investigated. No dose--effect dependence was detected when different concentrations of Cerebral were used. The administration of Cerebral as a neuroactivating remedy combined with piracetam and verapamil was most effective, leading to an increase in the lifespan and a delay in the appearance of brain degenerative processes.

[Neuroimmunocorrection therapy for the prophylaxis of infectious complications in acute stroke patients].

Neuroimmunocorrection therapy with cerebrolysin has been used for the prophylaxis of clinical pneumonia development in the early stage of acute stroke in a group of 140 patients with heavy clinical course of acute ischemic stroke (AIS). All patients in the test and control groups received the basal anti-AIS therapy and antibacterial drugs (IV-generation cephalosporins) in case of pneumonia development. The efficacy of cerebrolysin administration was evaluated both on the clinical scale (NIH-NINDS, CPIS, SIRS immonograms) and using laboratory indices.

[Rat immunoreactivity in experimental modeling of acute autohemorrhagic bihemispheric stroke in the presence of staphylococcal infection].

Experimental studies were made of rat immunoreactivity in experimental modeling of acute hemorrhagic stroke in staphylococcal infection. Bactericidal activity and reserve bactericidal activity of neutrophils in NBT-test enhance while mid-molecular CIC level decreases because of phagocyte activation. ABA high titers in blood reflect activation of autoimmune processes.

[Effects of neuropeptides on interferon production in vitro].

The study of an interferon-inducing action of neuropeptides (a cerebrolysin model) on production of interferons by human blood leukocytes has shown that neuropeptides induce gamma-interferon production in the titer 267 IU/ml that determines one of the mechanisms of a neuroimmunocorrecting effect of cerebrolysin (Ebewe, Austria) in many neurological diseases (acute stroke, brain traumas and different neuroinfectious diseases).

[Development of encephalitis in modeling of acute hemorrhagic stroke--a new variant of a somatic complication].

We studied immunological and morphological changes in rat brain in an acute period of experimental hemorrhagic stroke (HS). The latter was induced mechanically by the method of A. N.

[Complex influence of pharmacological agents on degenerative processes in brain of Drosophila melanogaster mutants].

A scheme of complex administration of drugs in the order neuroprotector + neuroactivator + neuroretarder for the treatment of neurodegenerative processes in the brain has been elaborated and tested on a model object representing neurodegenerative mutants of Drosophila melanogaster. The appearance of changes in the brain was delayed when drugs were used separately: donepezil hydrochloride (arisept), 10 - 11 days, epinephrine and nimodipine, 1 - 2 days. The treatment of flies with the same drugs in the order arisept + epinephrine + nimodipine leads to the complete regeneration of D.

[Degradation of p-toluenesulfonate by immobilized Comamonas testosteroni BS1310 (pBS1010) cells].

Parameters of degradation of p-toluenesulfonate (TS) by free and agar-embedded Comamonas testosteroni BS1310 (pBS1010) cells were determined. The maximum rate of TS degradation was 25% lower in by immobilized than free cells, equaling 11 nmol x min(-1) x mg(-1) cells. Degradation of TS by both free and immobilized cells was associated with molecular oxygen consumption (molar ratio, 1 : 2).

Virtual computational chemistry laboratory--design and description.

Internet technology offers an excellent opportunity for the development of tools by the cooperative effort of various groups and institutions. We have developed a multi-platform software system, Virtual Computational Chemistry Laboratory, http://www.vcclab.

Effect of active fraction of cerebral on expression of caspase-3 and beta-amyloid precursor protein during therapy of hemorrhagic stroke in the acute and delayed periods.

Active anti-stroke fraction of Cerebral preparation (extract of water-soluble molecules from brain tissue of animals with hemorrhagic stroke) decreased caspase-3 expression and improved survival of experimental animals in the acute period after hemorrhagic stroke.