Differences in thermal stability of the fetal and adult brain myoinositol-1-phosphate synthase. Probable involvement of NAD.

L-myo-inositol-1-phosphate synthase (EC 5.5.1.

Frequency analysis of adventitious lung sounds.

Adventitious lung sounds from twenty patients with respiratory diseases have been recorded and analysed by analogue methods. The results obtained are compared with those from five normal subjects. The difference in the frequency components of lung sounds from patients with pulmonary obstruction in the airways and those from normal subjects are pointed out.

The mechanism of interallelic complementation at the INO1 locus in yeast: immunological analysis of mutants.

The ino1 locus of yeast has been demonstrated to be the structural gene for the repressible enzyme, L-myo-inositol-1-phosphate synthase (Donahue and Henry 1981 a). We have screened a large number of allelic representatives of the ino1 locus for the presence of protein which cross reacts with antibody produced in response to purified wild type inositol-1-phosphate synthase. Approximately 50% of all ino1 representatives screened by immunoprecipitation produce a protein of 62,000 molecular weight, identical in size to the wild type enzyme subunit.

Unequivocal demonstration of fructose-1,6-bisphosphatase in mammalian brain.

Fructose-1,6-bisphosphatase (D-fructose-1,6-bisphosphate 1-phosphohydrolase; EC 3.1.3.

Iron and hemoglobin status of the offspring of guinea pigs whose mother received large doses of vitamin C during pregnancy and lactation.

1. Lower level of serum and liver iron were noted in the offspring of experimental group quinea pigs who received 1, 2 and 3 mg ascorbic acid per 100 g body weight per day. 2.

Effects of high intake of vitamin C by the guinea pigs in pregnancy and lactation on the tissue levels of the vitamin in their offspring.

1. Though the growth-rate of the guinea pigs of both the groups - control and experimental were almost same, the gain in body weight in the offspring of experimental group animals were observed to be less when they were fed 1, 2 and 3 mg ascorbic acid per 100 g body weight per day. 2.

Inhibition of glycolysis in brain by a phospholipid effect on interconversion of fructose phosphates. A possible regulatory control on utilization of glucose 6-phosphate.

Glucose 6-phosphate accumulation in 10,000 X g supernatant of rat brain was enhanced up to 16-fold by the addition of phosphatidylcholine, other common phospholipids, or linoleate. This glucose 6-phosphate is of endogenous origin via UDP-glucose and glucose 1-phosphate but not glucose. The accumulation is the result of inhibition of glycolysis by an effect of phospholipid on the interconversion of fructose 6-phosphate and fructose 1,6-bisphosphate.

Effect of ascorbic acid on histamine metabolism in scorbutic guinea-pigs.

1. In guinea-pigs fed an ascorbic-acid-free diet, as the ascorbic acid levels decreased the histamine levels in blood and urine rose steadily to maxima in about 10-12 days. The elevated histamine levels persisted in the blood and urine of scorbutic guinea-pigs and the histamine levels in lung, gastric mucosa and spleen also increased.

Nutrient interrelationship of ascorbic acid and iron in rats and guinea pigs fed cereal diets.

Iron at a daily dose of 5 mg per animal was toxic to rats and guinea pigs fed unfortified whole wheat flour and whole rice flour diets. In rats, a species capable of synthesizing ascorbic acid, iron supplementation resulted only in decreased growth. In guinea pigs, iron administration resulted not only in decreased growth but also in high mortality.

Growth and ascorbic acid metabolism in rats and guinea pigs fed cereal diets.

The nutritional values of the whole grain principal cereals, namely, wheat, rice, corn, and a millet bajra, were investigated by growth studies and studies on ascorbic acid metabolism in rats and guinea pigs. Growth was markedly retarded by feeding the rats unfortified whole grain cereals. Among the whole grain cereals, rice provided the lowest nutritional value, and the cooking of rice affected the nutritional value further.