The extracellular matrix in hepatocellular carcinoma shows different localization patterns depending on the differentiation and the histological pattern of tumors: immunohistochemical analysis.

This study investigated cells producing type I, III, and IV collagens, laminin, and fibronectin, the major components of the extracellular matrix, and compared their localization patterns in relation to the grade of tumor differentiation and the histological pattern of hepatocellular carcinoma. Type I, III, and IV collagens, laminin, and fibronectin were produced by tumor, endothelial, and Ito cells. Regarding their localization pattern in relation to the histological pattern of tumors, although the extracellular matrix was present in the subendothelial spaces of sinusoids in every histological pattern, the localization of these components in the intercellular spaces of tumor cells was most marked in hepatocellular carcinoma with a compact pattern.

Nasal mast cells in experimentally induced allergic rhinitis in guinea-pigs.

The kinetics of mast cell accumulation in the nasal mucosa during allergen exposure were investigated in the animal model of experimentally induced allergic rhinitis. Guinea-pigs were divided into an unsensitized control group and five sensitized groups each containing five animals. Five sensitized groups were immunized intraperitoneally with ovalbumin, followed by intranasal administration of ovalbumin in each of four groups for 1, 2, 3 and 4 weeks, respectively.

Distribution of substance P immunoreactive nerve fibers in the tracheal submucosal gland of cats.

Immunohistochemistry combined with electron microscopy was employed to investigate the distribution of substance P-immunoreactive (SP-IR) nerve fibers in the tracheal submucosal gland of cats. The SP-IR nerve fibers were found to form a network around the glands. Numerous varicosities were also detected within the basement membrane of the acini and secretory tubules.

[Syphilitic uveitis].

Three cases of syphilitic uveitis were described in which penicillin therapy combined with oral steroid regimen afforded rash disappearance of retinal edema in all cases. However, remarkable recuperation of vision was obtained in 2 cases.

Percutaneous ethanol injection therapy for recurrent hepatocellular carcinoma.

Recently, small hepatocellular carcinoma (HCC) has been detected by following up patients with chronic liver diseases using ultrasonography (US). Most of the HCC patients have liver cirrhosis, and multinodular lesions are often found, even in cases of small HCC. Thus, surgical resection for small HCC is of limited efficacy.

Serial changes in serum alpha-fetoprotein prior to detection of hepatocellular carcinoma in liver cirrhosis.

In 49 liver cirrhosis patients with hepatocellular carcinoma in which the hepatocellular carcinoma nodule was smaller than 30 mm in size, serial changes in serum alpha-fetoprotein levels prior to the detection of the hepatocellular carcinoma were studied retrospectively, and compared with those of 70 cirrhotic patients with no hepatocellular carcinoma. The changes in alpha-fetoprotein levels were classified into the 4 types normal, low plateau, high plateau and spiky type. The spiky type (spiky elevation during a short period) was more frequently noted in the patients with hepatocellular carcinoma than in those with liver cirrhosis.

Prevalence of gastroesophageal varices supplied by intrahepatic portal branch.

The contribution of the intrahepatic portal branch to the gastroesophageal varices is rare. This anomalous pathway (a large left intrahepatic portal branch) was proved by portographic study and by imaging techniques in a 54-year-old man with cirrhosis. The overall prevalence of this type of collateral in the literature is only 1.

Quantitative cytology of nasal secretions under various conditions.

Quantitative cytology was performed in nasal secretions of normal control (NC), seasonal allergic rhinitis in season (SAR), perennial allergic rhinitis (PAR), chronic sinusitis with mucoid secretion (MS), and chronic sinusitis with mucopurulent secretion (MPS). The majority of inflammatory cells were neutrophils in NC, MS, and MPS; the majority were eosinophils in SAR and PAR. The concomitant appearance of inflammatory cells in nasal secretions was found, i.

Effects of nasal allergen challenge on dynamic viscoelasticity of nasal mucus.

The effects of nasal provocation on the rheologic properties of nasal mucus were investigated in patients with allergic rhinitis provoked by house dust. The elastic modulus (G') and the dynamic viscosity (eta') of nasal mucus were determined by an oscillating sphere magnetic rheometer. Before and after the allergen challenge, G' increased, whereas eta' decreased with increasing oscillatory frequency; these findings indicate that the nasal mucus under these conditions is a non-newtonian fluid and has the cross-linked gel-like nature typical of mucus.

A patient with hepatocellular carcinoma who underwent resection of the primary lesion 10 years ago and resection of a giant adrenal metastasis 8 and a half years later.

A 56-year-old male consulted us because of a palpable mass and pain of the left flank 8 and a half years after resection of hepatocellular carcinoma of the left lobe about 3 cm in diameter. Ultrasound examination of the abdomen demonstrated a tumor about 10 cm in diameter showing a mosaic of hyperechoic and hypoechoic areas on the upper pole of the left kidney. By angiography, the tumor was found to be supplied mainly by the inferior adrenal artery.

Immunohistochemical and electron microscopic studies of substance P in guinea pig nasal glands.

The distribution of substance P-like immunoreactive (SP-IR) nerve endings in the nasal gland of the guinea pig was studied by using histochemical techniques to detect mucous glycoprotein and immunohistochemical techniques for SP, in combination with electron microscopy. Most nasal glands were negative for both AB and periodic acid-Schiff (PAS) staining. The SP-IR nerve fibers were found to form a network around these glands.

Nasal mucociliary transport of chronic sinusitis in children.

Nasal mucociliary function is one of the most important and indispensable mechanisms of the respiratory tract, providing protection against the atmospheric environment. We previously found mucociliary dysfunction in the noses of adult patients suffering from chronic sinusitis. In this study, using the saccharin method, we determined nasal mucociliary function in normal children and in children with chronic sinusitis.

The morphologic transition in hepatocellular carcinoma. A comparison of the individual histologic features disclosed by ultrasound-guided fine-needle biopsy with those of autopsy.

In hepatocellular carcinoma (HCC), it has been strongly suggested that as the tumor increases in size, foci of less-differentiated malignant tissues arise in the well-differentiated tumor and increase until they replace the well-differentiated tumor tissues. It has also been suggested that tumor growth is attributed to such dedifferentiation. In the current study, the individual histologic features of ultrasound-guided fine-needle biopsy specimens, taken from 12 small HCC in an early stage, were compared with those of autopsy to confirm the dedifferentiation of HCC.

[Clinical study on relationship between hepatocellular carcinoma and hepatitis C virus infection in patients with chronic liver disease].

The relationship between hepatocellular carcinoma (HCC) and hepatitis C virus (HCV) infection was investigated. Antibody to hepatitis C virus was detected in 88.8% and 87.

[Surgical treatments of adult chronic sinusitis--a study by questionnaire from 434 hospitals in Japan].

Information concerning surgical treatment of adult patients with chronic sinusitis was obtained through questionnaires from 434 hospitals in Japan. The hospitals were authorized for an in-service otorhinolaryngological training institute by the Oto-Rhino-Laryngological Society of Japan. The questionnaires were sent to the hospitals in December, 1989, and answers were obtained from 370 hospitals within 2 months, an 85.

Relationship between dynamic viscoelasticity and biochemical parameters in whole sputum from patients with hypersecretory respiratory diseases.

To obtain suitable biochemical parameters for measuring the dynamic viscoelasticity of sputum, we examined the relationships of the dynamic viscoelasticity with the dry weight (DW) and the contents of fucose, a parameter of airway mucous glycoprotein (AMG), and total protein (TP) and major proteins in whole sputum. The DW and TP, albumin and IgG concentrations and elastase activity were higher in purulent sputum than in mucoid-mucopurulent sputum, but there was no significant difference in the concentrations of airway wall-derived substances, such as fucose and S-IgA in the two. In mucoid-mucopurulent sputum, the dynamic viscosity (eta') and elastic modulus (G'), an indicator of dynamic elasticity, were most closely correlated with the fucose concentration (r = +0.

[Observation of cellular communication in the corneal endothelium using a small molecular weight fluorescent dye].

The metabolic and functional connection between corneal endothelial cells is thought to depend on the transfer of small molecules via gap junctions, as has been reported for other tissues. Cell-to-cell communication in the corneal endothelium was studied by monitoring the spread of fluorescence following the direct injection of Lucifer Yellow CH into single endothelial cells of the excised rabbit cornea. The image of the endothelial cells was observed during the injection and post-injection periods using specular microscopy and fluorescence microscopy.

[Effects of frequent antral lavage on chronic sinusitis in children].

Eighty cases of pediatric sinusitis (4 to 15 years old, averaged 8 years old) were treated with frequent antral irrigations. Antral puncture was made through the inferior meatus with a Kyoto University needle under topical or general anesthesia. Then a plastic tube of 14G Medicut intravenous cannula was left in place to serve as an indwelling catheter.

[Occurrence of optic disk hemorrhage in the course of Vogt-Koyanagi-Harada syndrome].

Cases, where in the course of Vogt-Koyanagi-Harada syndrome disc hemorrhages occur, are rare. One case was reported wherein about 2 weeks following the development of the disease, bilateral linear disc hemorrhages appeared and in 3 months disappeared, without sequellae of defective vision.

[Effect of recognition of head and neck malignancies on patients' psychosomatic condition and personality].

In order to elucidate the effect of recognition of head and neck malignancies on patients' psychosomatic condition and personality, we applied the Cornell Medical Index Health Questionnaire (CMI) and the Maudsley Personality Inventory (MPI) and collected medical and psychological data from 35 male (42-78 years of age; average 62.5) and 15 female (20-76 years of age; average 54.7) patients with malignant head and neck tumors (10 nose and paranasal, 8 oral, 8 pharyngeal, 14 laryngeal, 6 thyroid gland and 4 others).

[Conventional X-ray versus CT in diagnosis of chronic sinusitis in children].

The interpretation of conventional radiographic views for sinusitis in children has given rise to considerable controversy. Thirty-three children (66 sides of sinuses) aged from 4 to 15 years who were suspected of having chronic sinusitis were studied to determine the accuracy of conventional X-ray examination, comparing the results with those of CT. Coronal CT was taken after conventional X-ray examination (Waters and occipito-frontal views), and the time interval between these two examinations was 0 to 14 days (average 5.

[The inhibitory effect of gamma-glutamylcysteinylethyl ester (gamma-GCE) instillation on experimental diabetic cataract formation in rats].

The effect of the instillation of gamma-glutamylcysteinylethyl ester (gamma-GCE), which has been reported to function as a precursor of glutathione, on cataract formation was examined in rats in which diabetes had been induced by Streptozotocin (STZ). Three days after i.p.

Magnetic resonance imaging of small hepatocellular carcinoma.

Thirty-eight patients with small hepatocellular carcinomas (HCCs), size less than 20 mm, initially detected by ultrasound (US) and histologically confirmed, were examined by magnetic resonance (MR) imaging, computed tomographic (CT) scan, and angiography. MR imaging demonstrated HCC nodules in nine (75.0%) of 12 patients with tumors less than 10 mm in diameter and in 22 (84.