Isolation, Culture and Characterization of Human Sertoli Cells by Flow Cytometry: Development of Procedure.

Background: The sertoli cells in the testis create unique and safe environment to protect seminiferous tubules from auto antigens and invading pathogens. These cells produce the survival factor of the blood-testis barrier and produce special materials such as androgen binding proteins and contribute to the coordinated action of spermatogenesis. Given that the sertoli cells play an essential role in spermatogenesis and the lack of these cells leads to the disruption of spermatogenesis, it is necessary to investigate the behavior and performance of these cells.

The Expression of TLR2 and TLR3 in Sertoli Cells of Azoospermic Patients.

Objectives: Toll-like receptors (TLRs) on Sertoli cells are thought to have essential roles in sperm protection. This study was conducted to investigate the expression of TLR2 and TLR3 in Sertoli cells of men with azoospermia.

Materials And Methods: In this experimental study, testicular biopsies were taken from ten azoospermic men.