Effects of vitamins C and E in tris citric acid glucose extender on chilled semen quality of Kail ram during different storage times.

Mammalian sperm cells are highly vulnerable to lipid peroxidation by free radicals. Antioxidants such as vitamin E, and vitamin C neutralize the activity of free radicals and protect the sperm from reactive oxygen species. The present study was conducted to investigate the effects of vitamin C, vitamin E, and their combination in a Tris-based extender on the semen quality of Kail Ram.

The High Presence of HCMV pp71 Proteins, Correlate with P63 Expression in Pancreatic Cancer Tumor Tissue.

Objective: The purpose of this retrospective investigation was (1) to screen the existence of HCMV in pancreatic cancer tissues in relation to the histopathological grading system of such tumor tissues. (2) To evaluate the expression of the (P63) tumor suppressor gene in these tissues. (3) To find out the impact of the coexistence of (HCMV) along with the p63 on the occurring histopathological alterations.

Evaluation of serum soluble HLA-G levels post-recovery from COVID-19 and post-vaccination (Sinopharm and Pfizer-BioNTech).

Serum soluble HLA-G (sHLA-G) levels have been shown to be upregulated in COVID-19 patients. In this study, sHLA-G levels were examined in COVID-19 patients 14-21 days post-recovery (100 patients) and 80 uninfected controls. In addition, individuals vaccinated with Sinopharm or Pfizer-BioNTech (50 individuals each) were followed 21 days post-first dose and 21 days post-second dose.

Incidence of malarial infection and response to antimalarial drugs at Districts Lower Dir and Swat of Khyber Pakhtunkhwa, Pakistan.

Malaria is the leading cause of mortality and morbidity all over the world. Several antimalarial drugs are available for the treatment of malaria. The main objective of this study was to investigate the incidence of malarial infection and the use of prescribed antimalarial drugs.

Ethnomedicinal appraisal of plants used for the treatment of gastrointestinal complaints by tribal communities living in Diamir district, Western Himalayas, Pakistan.

Majority of the mountain dwelling communities living in the Himalayas rely on traditional herbal medicines for primary healthcare needs. Present study was conducted in fairy meadows and allied valleys in District Diamir, Gilgit Baltistan autonomous territory in northern Pakistan. Documentation of traditional medicinal knowledge (TMK) of local communities for the treatment of gastrointestinal disorders was carried out as a component of a wider medico-botanical expedition conducted in the entire base camp of the great Nanga Parbat peak during 2016-19.

Epidemiology of Ectoparasites (Ticks, Lice, and Mites) in the Livestock of Pakistan: A Review.

Ectoparasites, including lice, ticks, and mites, inhabit the host skin and depend on their host for sustenance, maturation, and multiplication. Among these, ticks are more prevalent in various regions of Pakistan because of favorable climatic conditions, lack of awareness of livestock keepers' regarding ectoparasite infestation rate, insufficient veterinary services, and inadequate control measures. Ectoparasitic infestation is a primary threat to cost-effective livestock production by damaging skin and transmitting multiple diseases between animals.

p53, p21, and cyclin d1 protein expression patterns in patients with breast cancer.

Background And Aim: The mutation in the wild-type tumor suppressor gene p53 is the most common genetic change in human tumors. In addition, the normal function of p21, which is both antiproliferative and an inhibitor of the cell cycle, is disrupted in some types of cancer. Meanwhile, cyclin D1 is a member of the cyclin protein family that is involved in regulating cell cycle progression.

What you need to know about children's COVID-19: a systematic review.

The aim of the systematic review is to describe clinical features of coronavirus disease 2019 (COVID-19) in children with a focus on the possible reasons why children are less susceptible to COVID-19 and whether their immune response works better than adults. The three research questions below were determined. (1) Why do pediatric COVID-19 cases have milder clinical symptoms than adults? (2) What are clinical manifestations, diagnosis, and treatment of COVID-19 in children? (3) How long lasts viral shedding after recovery? We searched MEDLINE, Embase, Cochrane Central Register of Controlled Trials, and Cochrane Database of Abstracts of Reviews of Effects, as well as foreign literature with English translations.

Transmission frequency of COVID-19 through pre-symptomatic and asymptomatic patients in AJK: a report of 201 cases.

Background: The COVID-19 pandemic is a catastrophic global phenomenon, affecting human life in a way unseen since the 1918 influenza pandemic. Effective management of this threat requires halting transmission, a strategy requiring accurate knowledge of SARS-CoV-2 transmission patterns.

Methods: This was a retrospective contact study aiming to estimate the transmission rate of COVID-19 by tracing contacts in symptomatic, pre-symptomatic, and asymptomatic patients.

Perception, preparedness and response of health care personals towards COVID-19 pandemic in Azad Jammu & Kashmir, Pakistan: A cross sectional interview based study.

Objective: To assess the state of knowledge, perception, preparedness and satisfaction level of health care personals (HCPs) in Azad Jammu & Kashmir (AJK) towards COVID-19.

Study Design: It was cross sectional survey conducted by personal interviews.

Methodology: The study was conducted by interviewing 302 HCPs from 7 major district headquarter hospitals of AJK from May to September 2020.

Biomonitoring of Toxic Metals in Feathers of Birds from North-Eastern Pakistan.

The current study was designed to determine the concentrations of toxic metals (Ni, Pb and Cr) in feathers of birds collected from four regions of NE Pakistan. Feather samples of birds (House Crow, Common Myna and House Sparrow) were collected from different areas. Atomic absorption spectrophotometer was used to determine the concentration of metals in feathers.

Identification of Potential HCV Inhibitors Based on the Interaction of Epigallocatechin-3-Gallate with Viral Envelope Proteins.

Hepatitis C is affecting millions of people around the globe annually, which leads to death in very high numbers. After many years of research, hepatitis C virus (HCV) remains a serious threat to the human population and needs proper management. The in silico approach in the drug discovery process is an efficient method in identifying inhibitors for various diseases.

Ethno-veterinary uses of Poaceae in Punjab, Pakistan.

Plant species of the Poaceae family are not only used as fodder and forage but also contribute substantially to the treatment of various health disorders, particularly in livestock. Consequently, the present study was aimed to document the therapeutic uses of Poaceae practiced by the inhabitants of the Punjab Province for the treatment of various veterinary health disorders. Semi structured interviews, group discussion and field walks were conducted to collect the data.

Systemic TNF-α blockade attenuates anxiety and depressive-like behaviors in mice through downregulation of inflammatory signaling in peripheral immune cells.

Research studies have indicated that the comorbidity burden of mood disorders and obesity is reasonably high. Insulin signaling has been shown to modulate multiple physiological functions in the brain, indicating its association with neuropsychiatric diseases, including mood disorders. Leptin is a hormone responsible for regulating body weight and insulin homeostasis.

Distribution and clinical significance of hepatitis B virus genotypes in Pakistan.

Background: Hepatitis B virus (HBV) genotype and its role in disease progression and patients' response to antiviral treatment, is not well studied in Pakistan. This comprehensive study was aimed to determine the distribution of HBV genotypes in Pakistan and their possible association with phases of HBV infection.

Methods: A total of 840 HBsAg positive samples was collected and tested for HBV DNA quantity.

Antiproliferative effects of aspirin and diclofenac against the growth of cancer and fibroblast cells: In vitro comparative study.

Non-steroidal anti-inflammatory drugs (NSAIDs) inhibit the growth of several cancer cell lines. The aim of this study is to compare the cytotoxic effect of aspirin with diclofenac on the growth of HeLa cell, mammary cell carcinoma, rhabdomyosarcoma and fibroblast cell lines in the culture media. The cells are cultured in RPMI-1640 culture media supplemented with 5% fetal calf serum and antibiotics.

Presence of HCV RNA in peripheral blood mononuclear cells may predict patients response to interferon and ribavirin therapy.

Background And Objectives: Hepatitis C virus (HCV) is considered a hepatotropic virus, but it can repli.cate in peripheral blood mononuclear cells (PBMCs), which influence the sustained virological response (SVR) of the patients, as well as relapse in successfully treated patients. The main objective of this study was to establish the importance of PBMC HCV RNA detection as a primary test to declare the patient as a responder, and the secondary objective was to investigate the risk of non-SVR or relapse in individuals who showed an end-of-treatment (ETR).

Nitrostative stress status during seasonal and pdmH1N1 infection in Iraq.

Introduction: Influenza A virus infection is associated with oxidative and nitrosative stress. This study aimed to assess nitrosative stress in pandemic H1N1 (pdmH1N1) and seasonal influenza A infected patients.

Methodology: The study included the following subjects:  20 patients infected with seasonal (negative one-step probe RT-PCR) influenza and 12 patients infected with pdmH1N1 (positive, one-step probe RT-PCR) influenza during the 2009 pandemic in Iraq.