[Measurements of DNA-ploidies in patients with carcinoma of the larynx].

In recent years there has been a lot of research done on using DNA-cytometry in the evaluation of larynx cancer. Everyone unanimously emphasizes that aneuploidal and polyploidal neoplasms require special therapeutical treatment. The definition of proliferating activity of neoplasm is of utmost importance.

Phase shifting the circadian clock with cycloheximide: response of hamsters with an intact or a split rhythm of locomotor activity.

Systemic administration of the protein synthesis inhibitor, cycloheximide, induced both phase advances and phase delays in the circadian rhythm of wheel-running activity in hamsters (Mesocricetus auratus) maintained in constant darkness or constant light. The magnitude and direction of the phase shifts were dependent on the circadian time (CT) of drug treatment. The phase response curves in constant darkness and constant light were of similar general shape, but they differed in the overall mean amplitude of the phase shifts.

The correlation of routine cytology with the contents of carcinoembryonic antigen and alpha-fetoprotein in pleural and peritoneal effusions.

The levels of carcinoembryonic antigen (CEA) and of alpha-fetoprotein (AFP) were determined with radioimmunoassay in 63 samples of either pleural or peritoneal effusions. All samples were collected from 53 patients suspected for malignancy and were also studied with the routine cytological techniques. Elevated levels of CEA (above 20 ng/ml) were found in 14 out of 29 cytologically positive samples, in none of 6 cytologically suspected and of 14 cytologically negative samples.

The direct radioimmunoassay technique for the determination of human alpha1-fetoprotein.

The direct radioimmunoassay procedure for the detection of human alpha-fetoprotein is described. The technique requires 20 microliter of sample for a single estimation and includes ammonium sulfate precipitation. The whole assay can be completed in 18 hours.