Oxidative damage in mitochondrial fatty acids oxidation disorders patients and the in vitro effect of l-carnitine on DNA damage induced by the accumulated metabolites.

Background: The mitochondrial fatty acids oxidation disorders (FAOD) are inherited metabolic disorders (IMD) characterized by the accumulation of fatty acids of different sizes of chain according to the affected enzyme.

Methods: This study evaluated the lipid peroxidation by the measurement of 8-isoprostanes, nitrosative stress parameters by the measurement of nitrite and nitrate content and DNA and RNA oxidative damage by the measurement of oxidized guanine species in urine samples from long-chain 3-hydroxyacyl-CoA dehydrogenase deficiency (LCHADD), medium-chain acyl-CoA dehydrogenase deficiency (MCADD) and multiple acyl-CoA dehydrogenase deficiency (MADD) patients. Also, we analyzed the in vitro DNA damage by comet assay induced by adipic acid, suberic acid, hexanoylglycine and suberylglycine, separated and in combination, as well as the effect of l-carnitine in human leukocytes.

Effects of Methanol Fraction from Leaves of Raddi on Nociception and Spinal-Cord Oxidative Biomarkers in Rats with Neuropathic Pain.

We determined the antioxidant potential of fractions obtained from leaves of , a medicinal plant known in Brazil as aroeira, to select the fraction with the best yield and antioxidant performance. These qualities were found in the methanol fraction (MeF), which was administered intraperitoneally (20 mg/kg/day) for 3 and 10 days to rats with chronic constriction injury (CCI) of the sciatic nerve, a model of neuropathic pain. The MeF increased the mechanical and thermal thresholds that had been lowered by CCI.

Peripheral oxidative stress blood markers in patients with chronic back or neck pain treated with high-velocity, low-amplitude manipulation.

Objective: The purpose of this study was to investigate oxidative-stress parameters in individuals with chronic neck or back pain after 5 weeks of treatment with high-velocity, low-amplitude (HVLA) spinal manipulation.

Methods: Twenty-three individuals aged 38.2 ± 11.

Effect of N-acetylcysteine on the spinal-cord glutathione system and nitric-oxide metabolites in rats with neuropathic pain.

Since N-acetylcysteine (NAC) is a donor of cysteine, we studied the relationship between NAC and concentration of oxidized and reduced glutathione (GSH/GSSG ratio), and glutathione peroxidase (GPx) and glutathione-S-transferase (GST) activities in the lumbosacral spinal cord of rats with chronic constriction injury (CCI) of the sciatic nerve that received NAC (150mg/kg/day, i.p.) or 0.

Assessment of oxidative parameters in rat spinal cord after chronic constriction of the sciatic nerve.

Although reactive oxygen species (ROS) are involved in neuropathic pain, the direct relationship between these species and chronic constriction of sciatic nerve (CCI) has not been studied in spinal cord. Thus, this study induced CCI in rats and these animals were sacrificed 3 and 10 days after the surgical procedure to determine the superoxide dismutase (SOD) and catalase activities, as well as ascorbic acid, hydrogen peroxide (H(2)O(2)) and lipid hydroperoxide levels in lumbosacral spinal cord. Von Frey Hair and hot plate tests were performed to assess the degree of mechanical and thermal hyperalgesia at days 0, 3 and 10.

Effect of high-velocity, low-amplitude treatment on superoxide dismutase and glutathione peroxidase activities in erythrocytes from men with neck pain.

Objective: This study investigates the analgesic effect of high-velocity, low-amplitude (HVLA) manipulation and antioxidant enzymes superoxide dismutase (SOD) and glutathione peroxidase (GPx) in erythrocytes of men with neck pain.

Methods: Twenty-two men with neck pain of mechanical origin who were aged 20 to 50 years, were nonsmokers, had a sedentary lifestyle, had no comorbidities, and were not in adjuvant therapy underwent 6 sessions of HVLA chiropractic manipulation 3 times a week for 2 weeks. Patients were treated by the same chiropractor and under the same conditions.

Prey selection by two benthic fish species in a Mato Grosso stream, Rio de Janeiro, Brazil.

Key to understand predator choice is the relationship between predator and prey abundance. There are few studies related to prey selection and availability. Such an approach is still current, because the ability to predict aspects of the diet in response to changes in prey availability is one of the major problems of trophic ecology.

Mesohabitat indicator species in a coastal stream of the Atlantic rainforest, Rio de Janeiro-Brazil.

The Mato Grosso is a typical Atlantic Forest stream located on the East coast of Brazil, approximately 70 km from Rio de Janeiro city. From its source at about 800m a.s.