A high-throughput assay identifies molecules with antimicrobial activity against persister cells.

Persister cells are transiently non-growing antibiotic-tolerant bacteria that cause infection relapse, and there is no effective antibiotic therapy to tackle these infections. High-throughput assays in drug discovery are biased towards detecting drugs that inhibit bacterial growth rather than killing non-growing bacteria. A new and simple assay to discover such drugs is needed.

Efficacy of rifampicin combination therapy against MRSA prosthetic vascular graft infections in a rat model.

is a major cause of prosthetic vascular graft or endograft infections (VGEIs) and the optimal choice of antibiotics is unclear. We investigated various antibiotic choices as either monotherapy or combination therapy with rifampicin against MRSA and . Fosfomycin, daptomycin and vancomycin alone or in combination with rifampicin was used against MRSA USA300 FPR3757.

Activation of the Two-Component System LisRK Promotes Cell Adhesion and High Ampicillin Tolerance in .

is a foodborne pathogen which can survive in harsh environmental conditions. It responds to external stimuli through an array of two-component systems (TCS) that sense external cues. Several TCS, including LisRK, have been linked to 's ability to grow at slightly elevated antibiotic levels.