Comparison of different versions of simulation studies following OECD 309 using C-labelled ionic compounds - Does it make a difference?

Simulation studies following OECD guideline 309 (Aerobic Mineralisation in Surface Water - Simulation Biodegradation Test) are performed to determine the biodegradation of chemicals in surface water. The aim of our study was to perform different versions of this test to identify possible shortcomings. In order to investigate the influence of a chemical charge on the degradation, we used C-labelled 4-n-dodecylbenzenesulfonic acid sodium salt (C-DS), 4-n-dodecylbenzyltrimethylammonium chloride (C-DA) and 4-n-dodecylphenol (C-DP) Additionally, abiotic degradation of these compounds and the effectiveness of different sterilization methods was assessed.