A repeated 18 bp sequence motif in the mouse rDNA spacer mediates binding of a nuclear factor and transcription termination.

DNA sequences and protein factors directing termination of mouse rDNA transcription in a nuclear extract system were examined. Termination is specific and requires a sequence element AGGTCGACCAGATTANTCCG (the Sall box) that is present eight times in the spacer region downstream of the 3' end of pre-rRNA. Exonuclease III protection experiments reveal the binding of a nuclear protein to the Sall box.

Mitomycin C plasma levels after intravesical instillation with and without hyaluronidase.

In 20 patients undergoing transurethral resection for bladder tumor or multiple transurethral biopsies for monitoring after transurethral resection, mitomycin C, 20 mg., was instilled into the bladder immediately after the procedure. Mitomycin C was associated with hyaluronidase, 200.

[Urologic complications following curative radiotherapy of gynecologic cancers].

Seventy-nine patients with urological complications after radiotherapy of their gynecological carcinomas are presented. Twenty-eight patients underwent operation before irradiation. The time between radiotherapy and occurrence of urological complications varied from 0.

Report of two cases of male breast cancer after prolonged estrogen treatment for prostatic carcinoma.

Reviewing our experience with 19 cases of male breast cancer, we found two patients with a 12-year history of estrogen therapy for prostatic carcinoma. One initially received diethylstilbene followed by estradiol, 80 mg monthly, and finally estramustin, 140 mg twice daily. The other patient had dienestrol, 2.

[Male breast cancer].

Between 1974 and 1982 inclusive 18 male patients were treated for breast carcinoma. 12 patients had postoperative radiotherapy whereas 4 were referred for treatment of recurrent or metastatic disease. One patient showed signs of inflammatory breast cancer and was treated with chemo-radiotherapy and one was being followed up in our department after radiotherapy for prostatic cancer in 1970.

Transcription of mouse rDNA terminates downstream of the 3' end of 28S RNA and involves interaction of factors with repeated sequences in the 3' spacer.

RNA polymerase I terminates transcription of mouse rDNA 565 bp downstream of the 3' end of mature 28S rRNA. This specific termination event can be duplicated in a nuclear extract system. RNA molecules with authentic 3' ends are transcribed from ribosomal minigene constructs provided the templates retain a minimal length of downstream spacer sequences.

[Significance of urothelial dysplasia in macroscopically normal mucosa in patients with bladder tumors. Results of a randomized study with and without intravesical mitomycin C in the prevention of recurrence].

During endoscopic treatment of 90 patients with bladder tumors, multiple random biopsies were carried out on normal looking bladder mucosa. Dysplasias were found in 25.6% of the patients.

[Testicular trauma and its significance for male fertility].

The clinical evaluation of 8 patients after surgical treatment of unilateral testicular trauma showed a decreased testicular volume in 3 patients. The serum endocrine profiles were within normal range--except 1 patient with atrophy of one testicle. In all ejaculates investigated (n = 6) a moderate to high asthenozoospermia was shown.

Pyridostigmine kinetics in healthy subjects and patients with myasthenia gravis.

Comparative pyridostigmine kinetics in plasma were measured in 10 healthy subjects given 4 mg iv and 60 mg oral pyridostigmine bromide. As determined from the AUC ratio, oral availability was 11.5% to 18.

[Therapy of virginal prostatic cancer with cyproterone acetate].

52 patients with virginal carcinoma of the prostate were treated with 100 mg cyproterone acetate during an observation period of 12 to 68 months (average 38.2 months). In 7 patients (13.

[Postoperative course following interventions for "septic kidney"].

It is reported on 61 patients who have been operated on for a "septic kidney". In 30 patients a primary nephrectomy was performed. One of these patients died immediately after the intervention in septic shock.

Long-term results after sclerotherapy of the spermatic vein in patients with varicocele.

Ninety-seven patients received transfemoral sclerotherapy of the spermatic vein for varicocele. After observation for 2 to 5 years, follow-up was available in 69 patients (71.2%), in 11 of whom the procedure failed (16.

Methylene blue to stain the bladder mucosa and monitor the course of bladder tumors.

In 12 patients with bladder tumors and in 15 undergoing multiple biopsies of grossly normal bladder mucosa to monitor the course of their bladder cancer, attempted staining of the mucosa with methylene blue failed to show any correlation with tumor histology. While histologically normal mucosal areas picked up the stain well, nonstaining areas were found to be dysplastic in 22% of the cases and showed endophytic masses in 2 patients. In 48% of patients excessive staining prevented adequate evaluation so that repeated endoscopy was necessary to confirm the absence of tumorous tissue.

[Dermatoglyphics in families with Potter type III polycystic kidney degeneration].

Several dermatoglyphic studies of patients suffering from genetically determined disorders which are not manifest at birth, indicate an early direct or indirect influence of the causative factors on the formation of dermatoglyphics. On the basis of these observations the present study gives an analysis of the quantitative and qualitative dermatoglyphic characteristics of fingers, palms and soles, as well as body and head measurements and morphological traits of the head and face of nine patients with adult polycystic kidney disease (APCD) type III and of their first and second degree relatives. No malformations nor any significant variation of these variables were observed, i.

[Initial experiences with the neodym YAG laser in urology].

The present study reports the therapeutic results obtained by using neodym-YAG laser for the coagulation of tumours of the external genitals (n = 30), as well as for endoscopic laser treatment of tumours of the bladder (n = 32) and the urethra (n = 2). Despite the small number of cases, laser treatment of bladder tumours (observation period 6-33 months) appears to reduce the orthotopic recurrence rate, which is in aggrement with other publications. However, randomized studies will be necessary to prove the advantages of the above-mentioned method as compared with electroresection.

The accuracy of transrectal aspiration biopsy of the prostate: an analysis of 452 cases.

This study compares cytological findings obtained by transrectal aspiration biopsy of the prostate with histological findings obtained by simultaneously performed transperineal punch biopsy. All interventions were performed on outpatients. Complications such as hematuria, urinary infection, or epididymitis occurred in 4.

[Demonstration of bacteria in the ejaculate of subfertile men, with special reference to Chlamydiae].

Bacterial cultures were obtained from ejaculates of 87 infertile males with pathologic spermiogram (according to the classification of Elliason), and the presence of chlamydia was also examined. In no case chlamydia could be proved. In only 8% bacteria could be found in a pathogenic number (greater than 10(4)).

[Significance of the incidental carcinoma of the prostate].

Incidental carcinoma of the prostate was detected in 58 patients after suprapubic prostatectomy for benign hypertrophy carried out in 942 men between 1962 and 1982. A 5-year follow-up was possible in 34 cases. The survival rate was assessed according to staging and grading of the carcinoma whereby the tumours were retrospectively reclassified according to Jewett.

[Drug prevention of recurrence in bladder tumors. Intravesical adriblastin instillation following transurethral resection of non-infiltrating bladder tumors].

29 patients were treated with intravesical adriamycin-instillations after transurethral resection of non-infiltrating bladder tumor. 50 mg Adriblastin were administered every four weeks during one year. In no case there were severe side-effects.

[Therapy of urinary tract infections with dibekacin].

The use of a new aminoglycosid antibiotic (dibekacin = Orbicin) in the treatment of severe urinary tract infections in a urological clinic is presented. After having given their written informed consent, 50 patients were treated with a dosage of 150-225 mg dibekacin, administered in three single daily doses. From a total of 41 mono-infections, asepsis could be attained in 70,7%, in 17,1% no effect could be observed and in 12,2% it came to a change of bacteria.

[Value of bladder washing cytology in the diagnosis and follow-up of bladder tumors].

It is reported on the accuracy of the cytological examination of freshly voided urine of 247 patients with 318 urothelial bladder tumors, and the results are compared with the evaluation of bladder washing cytology in 82 patients with 105 bladder tumors. If voided urine is used for examination, there is no evidence in tumors with 0 and 1 grade of malignancy, whereas the bladder washing cytology of grade 1 tumors shows a correlation for the histological findings in 26.3%.

Pregnancy rate after homologous insemination of pathological ejaculate both with and without the addition of kallikrein.

As part of a gynecological-andrological cooperation programme for the treatment of infertile marriages made by the 1st Department of Obstetrics and Gynecology and the Department of Urology, University of Vienna Medical School, the pregnancy rates achieved through 1,269 homologous inseminations of pathological ejaculates (according to Elliason's classification) both with and without addition of kallikrein was analysed retrospectively. In the case of 131 married couples, only 5 pregnancies occurred without the addition of kallikrein, and where kallikrein was used, there were also 5 pregnancies in 75 couples (6.66%).