[Effect of ribosomal proteins of respiratory bacteria on adhesive properties of buccal epitheliocytes].

Aim: Study the effect of ribosomal proteins of respiratory bacteria composing the basis of the immune-modulating preparation ribomunil on adhesive properties of buccal epithelium of healthy donors, and carry out comparison of this parameter during use of other bacterial products.

Materials And Methods: Various amounts of bacterial ribosomal proteins, Escherichia coli (serotype O127:B8) and one-day Staphylococcus aureus (strain 5983) culture supernatant were added to "buccal epitheliocytes--candida" system and incubated. Buccal cells were washed after the incubation from non-bound candida and differentiated microscopically by the amount of cells with various levels of candida adhesion.

[Neutrophils and bacterial biofilms: dialectics of relationship].

The review is dedicated to the problem of interaction of human neutrophils with bacterial biofilms that complicate the course of infectious process. Neutrophils being the most important effectors of innate immunity may attack bacterial biofilms causing their rejections and damage of biofilm microbes. Mechanisms of neutrophil-dependent destruction of biofilms are analyzed in the review.

Vesicle formation as a result of interaction between polymorphonuclear neutrophils and Staphylococcus aureus biofilm.

Staphylococcus aureus, a major opportunistic pathogen, is a leading cause of biofilm-related infections in clinical practice. Staphylococcal biofilms are highly resistant to antibacterial medicines and immune effector cells. The main result of our work is the discovery of nano-vesicles in the supernatant of the human neutrophil-S.

[Pseudomonas aeruginosa: characteristics of biofilm process].

Definition of the biofilm process as one of the types of intercellular bacterial communications is presented. The modern data concerning the structure of the Pseudomonas aeruginosa biofilm matrix and genetic mechanisms necessary for its production are described. Active and passive rejections of biofilm bacteria, which are the basis of bacterial spreading to new surfaces, are discussed.

[Interspecies interaction of bacteria and the formation of mixed (polymicrobial) biofilm].

Natural biofilms rarely exist as monocultures. Usually they are formed from various microorganism species that interact with each other, have shared metabolites, strengthen the attachment of each other to the support substrate, provide expression of "foreign" genes etc. Material on factors and mechanisms that determine the formation of mixed (polymicrobial) biofilms is analyzed in the review.

[Staphylococcal biofilms: structure, regulation, rejection].

Staphylococci are able to cause chronic (persistent) infections, which develop in native tissues as well as on invasive materials artificially introduced into an organism. Such infections are associated with formation of biofilms. The review determines the definition of biofilms, describes factors, which contribute to their formation, characterizes adaptive stability of staphylococcal biofilms, which provides their long-term persistence in host organism.

[Role of nuclear factor-kappaB in providing interactions of human neutrophils and epithelial cells from oral cavity with Candida albicans].

Contribution of intracellular signal pathways associated with nuclear factor kappaB (NF-kappaB) in realization of interactions of human neutrophils and epithelial cells from oral cavity with Candida albicans was studied. Supression of NF-kappaB in epitheliocytes resulted in decreased adsorption of C. albicans to buccal cells (buccal mucosa).

[Antiadhesive effect of the secretion of the oral cavity in the system "Candida albicans--buccal epitheliocytes"].

The influence of the oral cavity secretion on adhesive reactions in the system "C. albicans--buccal epitheliocytes" was studied. The treatment of C.

[Reactivity of Candida albicans in the system of the alternative way of complement activation].

The neutrophil-stimulating activity of C. albicans before and after opsonization in the system of the alternative way of complement activation (AWCA) was studied. The study revealed that, in comparison with zymosan, C.

[Influence of staphylococcal metabolites on adhesive reactions in the system "Candida albicans-buccal epitheliocytes"].

The influence of S. aureus and S. epidermitidis metabolites on the adhesive reactions in the system "C.

[A method for measuring the strength of adhesion of Candida albicans on epithelial cells].

A method on estimation of Candida albicans adhesion to epitheliocytes is proposed. The principle consists in the fact that while centrifuging epitheliocytes (1000 cycles/min, 10 min) with adhered Candida albicans through phycoll-verografin (1.077 g/cm3 density), Candida albicans cells having poor adhesion are separated from epitheliocytes trying to rise to the surface of phycoll-verografin.

[Phenomenon of selective decrease of colonization (adherence) resistance in the system "Candida albicans-buccal epithelial cells"].

In the period of the exacerbation of bronchial asthma an increased adhesiveness (in vitro) of buccal epithelial cells to C. albicans was noted in most children under study (94.7%).

[TT (TTV) Virus].

Characterization of TT virus (TTV), the history of its discovery, taxonomy and identification are reviewed as update on the diagnostics of TTV infection with PCR. The variability of the virus and resulting difficulties in the selection of a TTV DNA fragment for amplification are described. Data on the virus prevalence, replication and persistence are given.

[An opsonin-independent strain of Staphylococcus epidermidis].

Human neutrophils, subjected to stimulation under different conditions (phorbol myristate acetate, opsonized zymosan, formylmethionyl-leucinephenylalanine, nonopsonized staphylococci), produced a factor (denoted as clumping factor, or CF) with a capacity for highly selective clumping and opsonization of staphylococci. Out of 68 strains of different species of staphylococci, only a single strains (S.epidermidis) was sensitive of CF.

[Reactivity to staphylococcal peptidoglycan in the neutrophil-endothelium system].

The influence of S. aureus peptidoglycan on the process of adhesion in the neutrophil-endothelium system was studied. General regularities of this reaction, as well as the discrete action of peptidoglycan on each of its components were studied.

[Secretion of membrane-bound serine proteinases by polymorphonuclear leukocytes during adhesion to various types of receptor-dependent and receptor-independent sorbents].

A high level of the membrane-bound proteinase (LMP) secretion by human polymorphonuclear leukocytes (up to 680 nmol/min/ml with N-benzoyl-L-arg-EE as a substrate) was shown during the cell adhesion to receptor-dependent (immobilized aggregates of IgG and C3b) and receptor-independent (DEAE-Sephadex and polymethyl methacrylate) absorbents. Incubation medium contained 6.10(6) cells/ml.

[Changes in the adhesive capacity of the substrates during the IgG- and C3b-dependent adhesion of polymorphonuclear leukocytes].

The capacity of IgG-sepharose, C3b-sephadex and DEAE-sephadex to adsorb human neutrophils after repeated contacts with these cells was studied. A reduction of neutrophil adhesion was found only in experiments with receptor-dependent sorbents (IgG-sepharose, C3b-sephadex). The reduction of adsorption capacity of neutrophil IgG-sepharose was not due to products secreted by cells into the incubation medium or to IgG elimination from the sorbent surface.

[The manifestation of functional heterogeneity of the peptidoglycans in opsonophagocytic reactions].

The peptidoglycan activity of Staphylococcus aureus CCM 885 and Bifidobacterium bifidum 1 was studied in the opsonophagocytic system on the basis of the luminol-dependent chemiluminescence of human neutrophils. B. bifidum peptidoglycan was more active in direct interaction with neutrophils.

[Phagocytosis: problems and prospects].

The paper reviews the current status of the teaching of phagocytosis and its main trends of development, by making comments on the investigations of the receptor-dependent responses of phagocytes, their interaction with the endothelium, ways of realization of the effector potential of the phagosystem. It underlines an active role of phagocytes in general and partial pathology. A great emphasis is laid on the problem of cell conditioning (priming, deactivation) and its value in characterizing inflammatory complications and responses of the body.

[Effects of concanavalin A on different variants of adhesive reactions of human neutrophils].

The influence of Con A on human neutrophils adhesion in interactions with sepharose 4B, C3b-sephadex G-25, DEAE-sephadex A-25, polymethylmethacrylate was studied. In concentrations 25 mg/ml and more Con A completely inhibited IgG-dependent adhesion but had no influence on other types of adhesion reactions. The effect was due to Con A interaction with neutrophils and could not be reproduced by Con A pretreatment of IgG-sepharose.